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Department of Physiology and Biophysics, University of Iowa Iowa City, Iowa 52242
Address requests for reprints to: Dr. Richard A. Maurer, Department of Physiology and Biophysics, University of Iowa, Iowa City, Iowa 52242.
Abstract
To provide a hybridization probe for analysis of the regulation of rat gonadotropin subunit mRNA levels, an effort was made to isolate a cloned cDNA for the β-subunit of rat FSH (FSHβ). Using a cloned bovine FSHβ cDNA as a hybridization probe, a rat pituitary 
gt10 cDNA library was screened and a single, strongly hybridizing clone identified. The 874 base pair cDNA insert from this clone contains the complete sequence of rat FSHβ including an aminoterminal precursor segment. Hybridization of this cloned cDNA to rat pituitary RNA demonstrated the presence of an approximately 2.0 kilobase RNA species containing FSHβ sequences. Cloned rat cDNA was also used to demonstrate that estrogen treatment of ovariectomized female rats results in decreases in mRNA concentrations for FSHβ and the β-subunit of LH with somewhat smaller decreases in 
-subunit mRNA concentrations. Little or no change was detected in the mRNA for the β-subunit of TSH.
FOOTNOTES
This work was supported by National Institute of Health Grant AM-36407 and Research Career Development Award AM-00841.
Received for publication July 9, 1987. Accepted for publication August 17, 1987.
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