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SEQUENCES OF INTEREST: Molecular Cloning of the Complementary Deoxyribonucleic Acid for Human Thyroid Peroxidase

Departments of Medicine, University of California-San Francisco San Francisco, California 94143
Veteran's Administration Medical Center San Francisco, California 94121
Thyroid Study Unit, University of Chicago Chicago, Illinois 60637

Address requests for reprints to: Basil Rapoport, Veterans' Administration Medical Center, Metabolism (111F), 4150Clement Street, San Francisco, California 94143.

Abstract

Five overlapping cDNA clones representing the entire mRNA for human thyroid peroxidase (TPO) have been isolated from a human Graves' thyroid cDNA library. The cDNA sequence has been determined. Human TPO cDNA contains 3060 bases from the start of transcription to the beginning of the poly (A)tail at the 3'-end. The derived amino acid sequence of human TPO consists of 933 amino acids with amol wt of 102,937. The derived amino acid sequence contains five potential glycosylation sites (Asn-X-Ser/Thr), a probable transmembrane signal peptide sequence at the amino terminus, and a hydrophobicputative membrane-spanning region beginning 85 amino acid residues from the carboxyl terminal end. Comparison of the human TPO amino acid sequence to that of pig TPO shows strong homology extending from the amino terminus to within 44 amino acid residues of the carboxyl-terminus.

FOOTNOTES

This work was funded by NIH Grants AM-36182, AM-19289, and the Research Service of the Veterans' Administration.

Received for publication August 4, 1987. Accepted for publication September 8, 1987.

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