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Androgen Regulation of c-myc Messenger Ribonucleic Acid Levels in Rat Ventral Prostate

The Laboratories for Reproductive Biology and The Department of Pediatrics, University of North Carolina Medical School Chapel Hill, North Carolina 27599
The Departments of Biochemistry, University of North Carolina Medical School Chapel Hill, North Carolina 27599
The Departments of Surgery, University of North Carolina Medical School Chapel Hill North Carolina 27599

Address requests for reprints to: Dr. Frank S. French, Department of Pediatrics, Medical Research Building 206H, The University of North Carolina Medical School, Chapel Hill, North Carolina 27514.

Abstract

Androgens regulate growth of the rat ventral prostate gland. In a search for possible mediators of androgen stimulated growth we have studied c-myc proto-oncogene expression in ventral prostate after androgen withdrawal and replacement. Steady state levels of c-myc mRNA were determined by Northern hybridization and compared with mRNA levels for prostatein, the major androgen dependent protein of ventral prostate. C-myc mRNA in ventral prostate increased nearly 4-fold within 1 day and 6- to 7-fold within 2 days after castration. Administration of androgen at the time of castration prevented this increase in c-myc mRNA levels. Androgen treatment of 4-day castrate rats caused c-myc mRNA levels to decrease within 4 h. Cycloheximide increased c-myc mRNA severalfold within 2 h. The net increase in c-myc mRNA after cycloheximide treatment was greater in the castrate than in the noncastrate or in androgen-treated castrate rats. These results suggest that androgen may influence both transcription and turnover of c-myc mRNA. Prostatein C3 mRNA decreased rapidly after castration and increased after androgen treatment of the castrate but was only slightly influenced by cycloheximide. Steady state levels of c-myc mRNA were higher in the 10-day-old rat and decreased with age while prostatein C3 mRNA increased with age. In situ hybridization demonstrated that both c-myc and prostatein mRNAs are expressed in the epithelial cells of ventral prostate acinar glands. These data indicate that androgens regulate the expression of c-myc in the ventral prostate.

FOOTNOTES

This work was supported by NIH Research Grants HD-04466, P30-HD-18968, HD-16910, and CA-32458.

Received for publication June 29, 1987. Accepted for publication September 22, 1987.

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