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Plasma Hyperosmolality Increases G Protein and 3',5'-Cyclic Adenosine Monophosphate Synthesis in the Paraventricular and Supraoptic Nuclei

Laboratory of Cell Biology, National Institute of Mental Health Bethesda Maryland 20892

Address requests for reprints to: W. Scott Young III, Labortory of Cell Biology, National Institute of Mental Health, Building 36, Room 3A17, Bethesda, Maryland 20892.

Abstract

Hyperosmotic stimuli produce profound changes in cellular morphology and biosynthetic activities within the hypothalamic paraventricular and supraoptic nuclei (SON) of the rat. The mechanisms by which osmoreceptive signals are transduced within these nuclei are poorly understood. We examined several components of the cAMP-associated second messenger system after giving rats 2% saline to drink for one week, a strong hyperosmotic stimulus. We found that mRNA levels for both the stimulatory and inhibitory guanine-nucleotide binding protein -subunits were increased in the paraventricular nucleus and SON. In the SON, these changes were accompanied by increased basal cAMP levels, cholera toxin-stimulated adenylate cyclase, and G_s. Our results suggest that G_s levels are not saturated with respect to adenylate cyclase coupling and that osmoreception activates the cAMP second messenger system.

FOOTNOTES

^* Current address: Nova Pharmaceuticals Corp., 6200 Freeport Center, Baltimore, Maryland 21224.

Received for publication July 23, 1987. Accepted for publication October 6, 1987.

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