| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030
Sonic hedgehog (Shh) is a secreted morphogen that regulates dorso-ventral patterning within the neural tube during embryonic development. It is well established that Shh can induce motor-neuron differentiation that coincides with the appearance of specific motor-neuron markers including chicken ovalbumin upstream promoter-transcription factor II (COUP-TFII) and Isl1. However, the mechanism of Shh-induced signaling pathway in vertebrates is not clearly defined. In this report we have identified COUP-TFII as a target gene for Shh. In addition we have used a 1.6-kb region of the COUP-TFII promoter to identify a target element that mediates the Shh-induced activity. Extensive deletions introduced within this region have further enabled us to identify a novel sonic hedgehog response element (ShhRE) in the COUP-TFII promoter. Point mutations introduced within the ShhRE reveal some key nucleotides that are essential for protein(s)-binding activity. Finally, the ShhRE is capable of functioning as a true enhancer element and can mediate Shh-induced transactivation of reporter gene via a heterologous promoter.
This article has been cited by other articles:
 |
|
 |
|
  Z. Xu, S. Yu, C.-H. Hsu, J. Eguchi, and E. D. Rosen The orphan nuclear receptor chicken ovalbumin upstream promoter-transcription factor II is a critical regulator of adipogenesis PNAS, February 19, 2008; 105(7): 2421 - 2426. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Takamoto, L.-R. You, K. Moses, C. Chiang, W. E. Zimmer, R. J. Schwartz, F. J. DeMayo, M.-J. Tsai, and S. Y. Tsai COUP-TFII is essential for radial and anteroposterior patterning of the stomach Development, May 1, 2005; 132(9): 2179 - 2189. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. T. Lee, L. Li, N. Takamoto, J. F. Martin, F. J. DeMayo, M.-J. Tsai, and S. Y. Tsai The Nuclear Orphan Receptor COUP-TFII Is Required for Limb and Skeletal Muscle Development Mol. Cell. Biol., December 15, 2004; 24(24): 10835 - 10843. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Buttitta, R. Mo, C.-C. Hui, and C.-M. Fan Interplays of Gli2 and Gli3 and their requirement in mediating Shh-dependent sclerotome induction Development, December 22, 2003; 130(25): 6233 - 6243. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 V. Krishnan, T. L. Moore, Y. L. Ma, L. M. Helvering, C. A. Frolik, K. M. Valasek, P. Ducy, and A. G. Geiser Parathyroid Hormone Bone Anabolic Action Requires Cbfa1/Runx2-Dependent Signaling Mol. Endocrinol., March 1, 2003; 17(3): 423 - 435. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Takamoto, B. Zhao, S. Y. Tsai, and F. J. DeMayo Identification of Indian Hedgehog as a Progesterone-Responsive Gene in the Murine Uterus Mol. Endocrinol., October 1, 2002; 16(10): 2338 - 2348. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N Methot and K Basler An absolute requirement for Cubitus interruptus in Hedgehog signaling Development, January 3, 2001; 128(5): 733 - 742. [Abstract] [PDF]
|
|
|
|
|
|
F. Adam, T. Sourisseau, R. Métivier, Y. Le Page, C. Desbois, D. Michel, and G. Salbert COUP-TFI (Chicken Ovalbumin Upstream Promoter-Transcription Factor I) Regulates Cell Migration and Axogenesis in Differentiating P19 Embryonal Carcinoma Cells Mol. Endocrinol., December 1, 2000; 14(12): 1918 - 1933. [Abstract] [Full Text]
|
|
|
|
|
|
A Gallet, C Angelats, S Kerridge, and P. Therond Cubitus interruptus-independent transduction of the Hedgehog signal in Drosophila Development, January 12, 2000; 127(24): 5509 - 5522. [Abstract] [PDF]
|
|
|
|
 |
|
 F. A. Pereira, Y. Qiu, G. Zhou, M.-J. Tsai, and S. Y. Tsai The orphan nuclear receptor COUP-TFII is required for angiogenesis and heart development Genes & Dev., April 15, 1999; 13(8): 1037 - 1049. [Abstract] [Full Text]
|
|
|
|
|
|
F Muller, B Chang, S Albert, N Fischer, L Tora, and U Strahle Intronic enhancers control expression of zebrafish sonic hedgehog in floor plate and notochord Development, January 5, 1999; 126(10): 2103 - 2116. [Abstract] [PDF]
|
|
|
|
 |
|
 V. Krishnan, F. A. Pereira, Y. Qiu, C. Chen, P. A. Beachy, S. Y. Tsai, and M. Tsai Mediation of Sonic Hedgehog-Induced Expression of COUP-TFII by a Protein Phosphatase Science, December 12, 1997; 278(5345): 1947 - 1950. [Abstract] [Full Text]
|
|
|
|
 |
|
 G. Elberg, J. M. Gimble, and S. Y. Tsai Modulation of the Murine Peroxisome Proliferator-activated Receptor gamma 2 Promoter Activity by CCAAT/Enhancer-binding Proteins J. Biol. Chem., September 1, 2000; 275(36): 27815 - 27822. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
