help button home button Endocrine Society Molecular Endocrinology ENDO 08 Sessions Library
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hu, J.-F.
Right arrow Articles by Hoffman, A. R.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hu, J.-F.
Right arrow Articles by Hoffman, A. R.
Molecular Endocrinology 11 (13): 1891-1898
Copyright © 1997 by The Endocrine Society

Modulation of Igf2 Genomic Imprinting in Mice Induced by 5-Azacytidine, an Inhibitor of DNA Methylation

Ji-Fan Hu1, Pamela H. Nguyen1, Nga V. Pham, Thanh H. Vu and Andrew R. Hoffman

Medical Service and Geriatric Research Education and Clinical Center Veterans Affairs Palo Alto Health Care System, and Division of Endocrinology, Gerontology, and Metabolism Department of Medicine Stanford University Palo Alto, California 94304

The adjacent genes, insulin-like growth factor 2 (Igf2) and H19, are imprinted in both mouse and human. While Igf2 is expressed from the paternal allele, H19 is transcribed exclusively from the maternal allele. To explore the underlying mechanism of Igf2 and H19 imprinting, we studied the effect of DNA demethylation on allelic expression by injecting mice with the demethylating agent 5-azacytidine (5-aza-C). We observed a >=2-fold increase in the abundance of Igf2 mRNA in liver from treated mice compared with that of control mice. There was no significant change in Igf2 or H19 expression in brain. In the 5-aza-C-treated mice, there was dramatic modulation of Igf2 imprinting. In some tissues, Igf2 was expressed biallelically, while in other tissues, the paternal allele was silenced and the normally imprinted maternal allele was expressed, an example of allelic switching. There was no change in the normal biallelic pattern of Igf2 expression in brain. H19, on the other hand, remained imprinted in all tissues in mice treated with 5-aza-C. These results provide the first example of a pharmacological manipulation of genomic imprinting of an endogenous gene in vivo and further implicate DNA methylation as an important factor in maintaining the differential allelic expression of the Igf2 gene.




This article has been cited by other articles:


Home page
 Mol. Cell. Biol.Home page
T. Li, J.-F. Hu, X. Qiu, J. Ling, H. Chen, S. Wang, A. Hou, T. H. Vu, and A. R. Hoffman
CTCF Regulates Allelic Expression of Igf2 by Orchestrating a Promoter-Polycomb Repressive Complex 2 Intrachromosomal Loop
Mol. Cell. Biol., October 15, 2008; 28(20): 6473 - 6482.
[Abstract] [Full Text] [PDF]

Home page
 Hum Mol GenetHome page
R. A. Waterland, J.-R. Lin, C. A. Smith, and R. L. Jirtle
Post-weaning diet affects genomic imprinting at the insulin-like growth factor 2 (Igf2) locus
Hum. Mol. Genet., March 1, 2006; 15(5): 705 - 716.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. E. Fish, C. C. Matouk, A. Rachlis, S. Lin, S. C. Tai, C. D'Abreo, and P. A. Marsden
The Expression of Endothelial Nitric-oxide Synthase Is Controlled by a Cell-specific Histone Code
J. Biol. Chem., July 1, 2005; 280(26): 24824 - 24838.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
B.P. Enright, L.-Y. Sung, C.-C. Chang, X. Yang, and X.C. Tian
Methylation and Acetylation Characteristics of Cloned Bovine Embryos from Donor Cells Treated with 5-aza-2'-Deoxycytidine
Biol Reprod, April 1, 2005; 72(4): 944 - 948.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. Han, W. Xie, S. H. Kim, L. Yue, and J. DeJong
A Short Core Promoter Drives Expression of the ALF Transcription Factor in Reproductive Tissues of Male and Female Mice
Biol Reprod, September 1, 2004; 71(3): 933 - 941.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
B.P. Enright, C. Kubota, X. Yang, and X.C. Tian
Epigenetic Characteristics and Development of Embryos Cloned from Donor Cells Treated by Trichostatin A or 5-aza-2'-deoxycytidine
Biol Reprod, September 1, 2003; 69(3): 896 - 901.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
B. Viollet, M. Yaniv, and M. Pontoglio
Embryonic but Not Postnatal Reexpression of Hepatocyte Nuclear Factor 1{alpha} (HNF1{alpha}) Can Reactivate the Silent Phenylalanine Hydroxylase Gene in HNF1{alpha}-Deficient Hepatocytes
Mol. Cell. Biol., June 1, 2001; 21(11): 3662 - 3670.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
J.-F. Hu, J. Pham, I. Dey, T. Li, T. H. Vu, and A. R. Hoffman
Allele-Specific Histone Acetylation Accompanies Genomic Imprinting of the Insulin-Like Growth Factor II Receptor Gene
Endocrinology, December 1, 2000; 141(12): 4428 - 4435.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J.-F. Hu, H. Oruganti, T. H. Vu, and A. R. Hoffman
Tissue-Specific Imprinting of the Mouse Insulin-Like Growth Factor II Receptor Gene Correlates with Differential Allele-Specific DNA Methylation
Mol. Endocrinol., February 1, 1998; 12(2): 220 - 232.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
A. El Kharroubi, G. Piras, and C. L. Stewart
DNA Demethylation Reactivates a Subset of Imprinted Genes in Uniparental Mouse Embryonic Fibroblasts
J. Biol. Chem., March 16, 2001; 276(12): 8674 - 8680.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1997 by The Endocrine Society