help button home button Endocrine Society Molecular Endocrinology
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Woodson, K. G.
Right arrow Articles by Milbrandt, J.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Woodson, K. G.
Right arrow Articles by Milbrandt, J.
Right arrowPubmed/NCBI databases
*Gene*GEO Profiles
*HomoloGene*UniGene
Molecular Endocrinology 11 (2): 117-126
Copyright © 1997 by The Endocrine Society

Characterization of the Promoter of SF-1, an Orphan Nuclear Receptor Required for Adrenal and Gonadal Development

Karen G. Woodson1, Peter A. Crawford1, Yoel Sadovsky1 and Jeffrey Milbrandt

Division of Laboratory Medicine Departments of Pathology and Internal Medicine (K.G.W., P.A.C., J.M.) Department of Obstetrics and Gynecology (Y.S.) Washington University School of Medicine St. Louis, Missouri 63110

Steroidogenic factor 1 (SF-1) is a transcription factor shown to be critical for regulation of adrenal and gonadal development and function. To dissect the mechanisms that direct expression of this regulator, we have studied the promoter of the SF-1 gene and have identified cis-acting elements that recognize a basic-helix-loop-helix transcription factor; the CAAT binding factor; and Sp1. We demonstrate in Y1 adrenocortical cells that a 90-bp proximal promoter fragment is sufficient to direct steroidogenic-specific expression and that all three elements are required for activity of the SF-1 promoter. Functional analysis of the binding sites on a heterologous TATA box-containing promoter demonstrates that the CAAT box and Sp1 site are not essential for promoter activity when a TATA box is present, whereas the E box is absolutely required for gene expression and is most likely the steroidogenic cell-specific element. We also demonstrate that SF-1 itself does not significantly affect the transcription of its own gene, and thus conclude that the E box, CAAT box, and Sp1 site of the proximal promoter direct expression of the SF-1 gene.




This article has been cited by other articles:


Home page
 Mol. Endocrinol.Home page
C. Andreu-Vieyra, R. Chen, and M. M. Matzuk
Conditional Deletion of the Retinoblastoma (Rb) Gene in Ovarian Granulosa Cells Leads to Premature Ovarian Failure
Mol. Endocrinol., September 1, 2008; 22(9): 2141 - 2161.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
H. Utsunomiya, Y.-H. Cheng, Z. Lin, S. Reierstad, P. Yin, E. Attar, Q. Xue, G. Imir, S. Thung, E. Trukhacheva, et al.
Upstream Stimulatory Factor-2 Regulates Steroidogenic Factor-1 Expression in Endometriosis
Mol. Endocrinol., April 1, 2008; 22(4): 904 - 914.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
W.-Y. Chen, J.-H. Weng, C.-C. Huang, and B.-c. Chung
Histone Deacetylase Inhibitors Reduce Steroidogenesis through SCF-Mediated Ubiquitination and Degradation of Steroidogenic Factor 1 (NR5A1)
Mol. Cell. Biol., October 15, 2007; 27(20): 7284 - 7290.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
W. Zheng, J. Yang, Q. Jiang, Z. He, and L. M Halvorson
Liver receptor homologue-1 regulates gonadotrope function
J. Mol. Endocrinol., February 1, 2007; 38(2): 207 - 219.
[Abstract] [Full Text] [PDF]

Home page
 BloodHome page
Y. Katoh-Fukui, A. Owaki, Y. Toyama, M. Kusaka, Y. Shinohara, M. Maekawa, K. Toshimori, and K.-i. Morohashi
Mouse Polycomb M33 is required for splenic vascular and adrenal gland formation through regulating Ad4BP/SF1 expression
Blood, September 1, 2005; 106(5): 1612 - 1620.
[Abstract] [Full Text] [PDF]

Home page
 J Mol EndocrinolHome page
J Liu, X-D Li, A Vaheri, and R Voutilainen
DNA methylation affects cell proliferation, cortisol secretion and steroidogenic gene expression in human adrenocortical NCI-H295R cells
J. Mol. Endocrinol., December 1, 2004; 33(3): 651 - 662.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. P. Scherrer, D. A. Rice, and L. L. Heckert
Expression of Steroidogenic Factor 1 in the Testis Requires an Interactive Array of Elements Within Its Proximal Promoter
Biol Reprod, November 1, 2002; 67(5): 1509 - 1521.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
N. R. Stallings, N. A. Hanley, G. Majdic, L. Zhao, M. Bakke, and K. L. Parker
Development of a Transgenic Green Fluorescent Protein Lineage Marker for Steroidogenic Factor 1
Mol. Endocrinol., October 1, 2002; 16(10): 2360 - 2370.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
D. Wilhelm and C. Englert
The Wilms tumor suppressor WT1 regulates early gonad development by activation of Sf1
Genes & Dev., July 15, 2002; 16(14): 1839 - 1851.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. H.-C. Shen and H. A. Ingraham
Regulation of the Orphan Nuclear Receptor Steroidogenic Factor 1 by Sox Proteins
Mol. Endocrinol., March 1, 2002; 16(3): 529 - 540.
[Abstract] [Full Text] [PDF]

Home page
 Recent Prog Horm ResHome page
K. L. Parker, D. A. Rice, D. S. Lala, Y. Ikeda, X. Luo, M. Wong, M. Bakke, L. Zhao, C. Frigeri, N. A. Hanley, et al.
Steroidogenic Factor 1: an Essential Mediator of Endocrine Development
Recent Prog. Horm. Res., January 1, 2002; 57(1): 19 - 36.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. S. Richards
Perspective: The Ovarian Follicle--A Perspective in 2001
Endocrinology, June 1, 2001; 142(6): 2184 - 2193.
[Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
L. L. Heckert
Activation of the Rat Follicle-Stimulating Hormone Receptor Promoter by Steroidogenic Factor 1 Is Blocked by Protein Kinase A and Requires Upstream Stimulatory Factor Binding to a Proximal E Box Element
Mol. Endocrinol., May 1, 2001; 15(5): 704 - 715.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
K. Yamada, T. Mizutani, Z. Shou, T. Yazawa, T. Sekiguchi, M. Yoshino, T. Inazu, and K. Miyamoto
Cloning and Functional Expression of an E Box-Binding Protein from Rat Granulosa Cells
Biol Reprod, May 1, 2001; 64(5): 1315 - 1319.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
J. J. Tremblay and R. S. Viger
GATA Factors Differentially Activate Multiple Gonadal Promoters through Conserved GATA Regulatory Elements
Endocrinology, March 1, 2001; 142(3): 977 - 986.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. R. Manna, J. Kero, M. Tena-Sempere, P. Pakarinen, D. M. Stocco, and I. T. Huhtaniemi
Assessment of Mechanisms of Thyroid Hormone Action in Mouse Leydig Cells: Regulation of the Steroidogenic Acute Regulatory Protein, Steroidogenesis, and Luteinizing Hormone Receptor Function
Endocrinology, January 1, 2001; 142(1): 319 - 331.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Higa, H. Kanda, T. Kitahashi, M. Ito, T. Shiba, and H. Ando
Quantitative Analysis of fushi tarazu Factor 1 Homolog Messenger Ribonucleic Acids in the Pituitary of Salmon at Different Prespawning Stages
Biol Reprod, December 1, 2000; 63(6): 1756 - 1763.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
M. A.F. Daggett, D. A. Rice, and L. L. Heckert
Expression of Steroidogenic Factor 1 in the Testis Requires an E Box and CCAAT Box in its Promoter Proximal Region
Biol Reprod, March 1, 2000; 62(3): 670 - 679.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
E. Barnea and Y. Bergman
Synergy of SF1 and RAR in Activation of Oct-3/4 Promoter
J. Biol. Chem., February 25, 2000; 275(9): 6608 - 6619.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
K. Kawabe, T. Shikayama, H. Tsuboi, S. Oka, K. Oba, T. Yanase, H. Nawata, and K.-i. Morohashi
Dax-1 as One of the Target Genes of Ad4BP/SF-1
Mol. Endocrinol., August 1, 1999; 13(8): 1267 - 1284.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
N. Pilon, R. Behdjani, I. Daneau, J. G. Lussier, and D. W. Silversides
Porcine Steroidogenic Factor-1 Gene (pSF-1) Expression and Analysis of Embryonic Pig Gonads during Sexual Differentiation
Endocrinology, September 1, 1998; 139(9): 3803 - 3812.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Cell. Biol.Home page
P. A. Crawford, C. Dorn, Y. Sadovsky, and J. Milbrandt
Nuclear Receptor DAX-1 Recruits Nuclear Receptor Corepressor N-CoR to Steroidogenic Factor 1
Mol. Cell. Biol., May 1, 1998; 18(5): 2949 - 2956.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
A. N. Harris and P. L. Mellon
The Basic Helix-Loop-Helix, Leucine Zipper Transcription Factor, USF (Upstream Stimulatory Factor), Is a Key Regulator of SF-1 (Steroidogenic Factor-1) Gene Expression in Pituitary Gonadotrope and Steroidogenic Cells
Mol. Endocrinol., May 1, 1998; 12(5): 714 - 726.
[Abstract] [Full Text]

Home page
 DevelopmentHome page
R. Viger, C Mertineit, J. Trasler, and M Nemer
Transcription factor GATA-4 is expressed in a sexually dimorphic pattern during mouse gonadal development and is a potent activator of the Mullerian inhibiting substance promoter
Development, January 7, 1998; 125(14): 2665 - 2675.
[Abstract] [PDF]

Home page
 Reproductive SciencesHome page
Y. Sadovsky and P. A. Crawford
Developmental and Physiologic Roles of the Nuclear Receptor Steroidogenic Factor-I in the Reproductive System
Reproductive Sciences, January 1, 1998; 5(1): 6 - 12.
[Abstract] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1997 by The Endocrine Society