help button home button Endocrine Society Molecular Endocrinology ENDO 08 Sessions Library
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Byers, M.
Right arrow Articles by Park-Sarge, O.-K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Byers, M.
Right arrow Articles by Park-Sarge, O.-K.
Molecular Endocrinology 11 (2): 172-182
Copyright © 1997 by The Endocrine Society

Estrogen Receptor-ß mRNA Expression in Rat Ovary: Down-Regulation by Gonadotropins

Michael Byers, George G. J. M. Kuiper, Jan-Åke Gustafsson and Ok-Kyong Park-Sarge

Department of Physiology (M.B., O-K.P-S.) University of Kentucky Lexington, Kentucky 40536
Center for Biotechnology (G.G.J.M.K.) and Department of Medical Nutrition (J-Å.G.) Karolinska Institute S-14186 Huddinge, Sweden

We have examined the expression and regulation of the two estrogen receptor (ER{alpha} and ERß) genes in the rat ovary, using Northern blotting, RT-PCR, and in situ hybridization histochemistry. Northern blotting results show that the ovary expresses both ER{alpha} and ERß genes as single (~6.5-kb) and multiple (ranging from ~1.0-kb to ~10.0-kb) transcripts, respectively. ER{alpha} mRNA is expressed at a level lower than ERß mRNA in immature rat ovaries. This relationship appears unchanged between sexually mature adult rats and immature rats. In sexually mature adult rats undergoing endogenous hormonal changes, whole ovarian content of ERß mRNA, as determined by RT-PCR, remained more or less constant with the exception of the evening of proestrus when ERß mRNA levels were decreased. Examination of ERß mRNA expression at the cellular level, by in situ hybridization, showed that ERß mRNA is expressed preferentially in granulosa cells of small, growing, and preovulatory follicles, although weak expression of ERß mRNA was observed in a subset of corpora lutea, and that the decrease in ERß mRNA during proestrous evening is attributable, at least in part, to down-regulation of ERß mRNA in the preovulatory follicles. This type of expression and regulation was not typical for ER{alpha} mRNA in the ovary. Although whole ovarian content of ER{alpha} mRNA was clearly detected by RT-PCR, no apparent modulation of ER{alpha} mRNA levels was observed during the estrous cycle. Examination of ER{alpha} mRNA expression at the cellular level, by in situ hybridization, showed that ER{alpha} mRNA is expressed at a low level throughout the ovary with no particular cellular localization.

To further examine the potential role of the preovulatory pituitary gonadotropins in regulating ERß mRNA expression in the ovary, we used immature rats treated with gonadotropins. In rats undergoing exogenous hormonal challenges, whole ovarian content of ERß mRNA, as determined by RT-PCR, remained more or less unchanged after an injection of PMSG. In contrast, a subsequent injection of human CG (hCG) resulted in a substantial decrease in whole ovarian content of ERß mRNA. In situ hybridization for ERß mRNA shows that small, growing, and preovulatory follicles express ERß mRNA in the granulosa cells. The preovulatory follicles contain ERß mRNA at a level lower than that observed for small and growing follicles. In addition, there is an abrupt decrease in ERß mRNA expression in the preovulatory follicles after hCG injection. The inhibitory effect of hCG on ERß mRNA expression was also observed in cultured granulosa cells. Moreover, agents stimulating LH/CG receptor-associated intracellular signaling pathways (forskolin and a phorbol ester) readily mimicked the effect of hCG in down-regulating ERß mRNA in cultured granulosa cells.

Taken together, our results demonstrate that 1) the ovary expresses both ER{alpha} and ERß genes, although ERß is the predominant form of estrogen receptor in the ovary, 2) ERß mRNA is localized predominantly to the granulosa cells of small, growing, and preovulatory follicles, and 3) the preovulatory LH surge down-regulates ERß mRNA. These results clearly implicate the physiological importance of ERß in female reproductive functions.




This article has been cited by other articles:


Home page
 Poult. Sci.Home page
B. Kang, J. R. Guo, H. M. Yang, R. J. Zhou, J. X. Liu, S. Z. Li, and C. Y. Dong
Differential expression profiling of ovarian genes in prelaying and laying geese
Poult. Sci., September 1, 2009; 88(9): 1975 - 1983.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
T. da Silva Faria, F. de Bittencourt Brasil, F. J B Sampaio, and C. da Fonte Ramos
Maternal malnutrition during lactation alters the folliculogenesis and gonadotropins and estrogen isoforms ovarian receptors in the offspring at puberty
J. Endocrinol., September 1, 2008; 198(3): 625 - 634.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
Y.-J. Chen, M.-T. Lee, H.-C. Yao, P.-W. Hsiao, F.-C. Ke, and J.-J. Hwang
Crucial Role of Estrogen Receptor-{alpha} Interaction with Transcription Coregulators in Follicle-Stimulating Hormone and Transforming Growth Factor {beta}1 Up-Regulation of Steroidogenesis in Rat Ovarian Granulosa Cells
Endocrinology, September 1, 2008; 149(9): 4658 - 4668.
[Abstract] [Full Text] [PDF]

Home page
 ReproductionHome page
S. van den Driesche, V. M Smith, M. Myers, and W C. Duncan
Expression and regulation of oestrogen receptors in the human corpus luteum
Reproduction, April 1, 2008; 135(4): 509 - 517.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. Ikeda, K. Nakamura, K. Kogure, Y. Omori, S. Yamashita, K. Kubota, T. Mizutani, K. Miyamoto, and T. Minegishi
Effect of Estrogen on the Expression of Luteinizing Hormone-Human Chorionic Gonadotropin Receptor Messenger Ribonucleic Acid in Cultured Rat Granulosa Cells
Endocrinology, April 1, 2008; 149(4): 1524 - 1533.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
C. Leigh Pearce, A. M. Near, J. L. Butler, D. Van Den Berg, P. Bretsky, D. V. Conti, D. O. Stram, M. C. Pike, J. N. Hirschhorn, and A. H. Wu
Comprehensive Evaluation of ESR2 Variation and Ovarian Cancer Risk
Cancer Epidemiol. Biomarkers Prev., February 1, 2008; 17(2): 393 - 396.
[Abstract] [Full Text] [PDF]

Home page
 Mol Hum ReprodHome page
K. S. Monkkonen, R. Aflatoonian, K.-F. Lee, W. S.B. Yeung, S.-W. Tsao, J. T. Laitinen, and A. Fazeli
Hormonal regulation of G{alpha}i2 and mPR{alpha} in immortalized human oviductal cell line OE-E6/E7
Mol. Hum. Reprod., December 1, 2007; 13(12): 845 - 851.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
C. Stocco, C. Telleria, and G. Gibori
The Molecular Control of Corpus Luteum Formation, Function, and Regression
Endocr. Rev., February 1, 2007; 28(1): 117 - 149.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. K. Woodruff and K. E. Mayo
To {beta} or Not To {beta}: Estrogen Receptors and Ovarian Function
Endocrinology, August 1, 2005; 146(8): 3244 - 3246.
[Full Text] [PDF]

Home page
 EndocrinologyHome page
J. F. Couse, M. M. Yates, B. J. Deroo, and K. S. Korach
Estrogen Receptor-{beta} Is Critical to Granulosa Cell Differentiation and the Ovulatory Response to Gonadotropins
Endocrinology, August 1, 2005; 146(8): 3247 - 3262.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
S. A. Milne, T. A. Henderson, R. W. Kelly, P. T. Saunders, D. T. Baird, and H. O. D. Critchley
Leukocyte Populations and Steroid Receptor Expression in Human First-Trimester Decidua; Regulation by Antiprogestin and Prostaglandin E Analog
J. Clin. Endocrinol. Metab., July 1, 2005; 90(7): 4315 - 4321.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
L. J. Spicer
Effects of Estradiol on Bovine Thecal Cell Function In Vitro: Dependence on Insulin and Gonadotropins
J Dairy Sci, July 1, 2005; 88(7): 2412 - 2421.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. M. A. Emmen, J. F. Couse, S. A. Elmore, M. M. Yates, G. E. Kissling, and K. S. Korach
In Vitro Growth and Ovulation of Follicles from Ovaries of Estrogen Receptor (ER){alpha} and ER{beta} Null Mice Indicate a Role for ER{beta} in Follicular Maturation
Endocrinology, June 1, 2005; 146(6): 2817 - 2826.
[Abstract] [Full Text] [PDF]

Home page
 J. Physiol.Home page
M. J. Byers, A. Zangl, T. M. Phernetton, G. Lopez, D.-b. Chen, and R. R. Magness
Endothelial vasodilator production by ovine uterine and systemic arteries: ovarian steroid and pregnancy control of ER{alpha} and ER{beta} levels
J. Physiol., May 15, 2005; 565(1): 85 - 99.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. E. Baker, V. M. Brautigam, and J. J. Watters
Estrogen Modulates Microglial Inflammatory Mediator Production via Interactions with Estrogen Receptor {beta}
Endocrinology, November 1, 2004; 145(11): 5021 - 5032.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
M. Jo, M. C. Gieske, C. E. Payne, S. E. Wheeler-Price, J. B. Gieske, I. V. Ignatius, T. E. Curry Jr., and C. Ko
Development and Application of a Rat Ovarian Gene Expression Database
Endocrinology, November 1, 2004; 145(11): 5384 - 5396.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
A Bardin, N Boulle, G Lazennec, F Vignon, and P Pujol
Loss of ER{beta} expression as a common step in estrogen-dependent tumor progression
Endocr. Relat. Cancer, September 1, 2004; 11(3): 537 - 551.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A.C.O. Evans, J.L.H. Ireland, M.E. Winn, P. Lonergan, G.W. Smith, P.M. Coussens, and J.J. Ireland
Identification of Genes Involved in Apoptosis and Dominant Follicle Development During Follicular Waves in Cattle
Biol Reprod, May 1, 2004; 70(5): 1475 - 1484.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol SciHome page
M. S. Golub, C. E. Hogrefe, S. L. Germann, B. L. Lasley, K. Natarajan, and A. F. Tarantal
Effects of Exogenous Estrogenic Agents on Pubertal Growth and Reproductive System Maturation in Female Rhesus Monkeys
Toxicol. Sci., July 1, 2003; 74(1): 103 - 113.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
S. B. Pillai, J. M. Jones, and R. D. Koos
Treatment of Rats with 17{beta}-Estradiol or Relaxin Rapidly Inhibits Uterine Estrogen Receptor {beta}1 and {beta}2 Messenger Ribonucleic Acid Levels
Biol Reprod, December 1, 2002; 67(6): 1919 - 1926.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
N. S. Stachenfeld and D. L. Keefe
Estrogen effects on osmotic regulation of AVP and fluid balance
Am J Physiol Endocrinol Metab, October 1, 2002; 283(4): E711 - E721.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
W. N. Jefferson, J. F. Couse, E. Padilla-Banks, K. S. Korach, and R. R. Newbold
Neonatal Exposure to Genistein Induces Estrogen Receptor (ER){alpha} Expression and Multioocyte Follicles in the Maturing Mouse Ovary: Evidence for ER{beta}-Mediated and Nonestrogenic Actions
Biol Reprod, October 1, 2002; 67(4): 1285 - 1296.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
E. Sapi, W. D. Brown, S. Aschkenazi, C. Lim, A. Munoz, B. M. Kacinski, T. Rutherford, and G. Mor
Regulation of Fas Ligand Expression By Estrogen in Normal Ovary
Reproductive Sciences, July 1, 2002; 9(4): 243 - 250.
[Abstract] [PDF]

Home page
 EndocrinologyHome page
D. Lopez, M. D. Sanchez, W. Shea-Eaton, and M. P. McLean
Estrogen Activates the High-Density Lipoprotein Receptor Gene via Binding to Estrogen Response Elements and Interaction with Sterol Regulatory Element Binding Protein-1A
Endocrinology, June 1, 2002; 143(6): 2155 - 2168.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
P. Yang, A. Kriatchko, and S. K. Roy
Expression of ER-{alpha} and ER-{beta} in the Hamster Ovary: Differential Regulation by Gonadotropins and Ovarian Steroid Hormones
Endocrinology, June 1, 2002; 143(6): 2385 - 2398.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. A. LaVoie, D. C. DeSimone, C. Gillio-Meina, and Y. Y. Hui
Cloning and Characterization of Porcine Ovarian Estrogen Receptor {beta} Isoforms
Biol Reprod, March 1, 2002; 66(3): 616 - 623.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
A. A. Kowalski, L. G. Graddy, D. S. Vale-Cruz, I. Choi, B. S. Katzenellenbogen, F. A. Simmen, and R. C.M. Simmen
Molecular Cloning of Porcine Estrogen Receptor-{beta} Complementary DNAs and Developmental Expression in Periimplantation Embryos
Biol Reprod, March 1, 2002; 66(3): 760 - 769.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. Frasor, K. Park, M. Byers, C. Telleria, T. Kitamura, L.-y. Yu-Lee, J. Djiane, O.-K. Park-Sarge, and G. Gibori
Differential Roles for Signal Transducers and Activators of Transcription 5a and 5b in PRL Stimulation of ER{alpha} and ER{beta} Transcription
Mol. Endocrinol., December 1, 2001; 15(12): 2172 - 2181.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
B. Greco, E. A. Allegretto, M. J. Tetel, and J. D. Blaustein
Coexpression of ER{beta} with ER{alpha} and Progestin Receptor Proteins in the Female Rat Forebrain: Effects of Estradiol Treatment
Endocrinology, December 1, 2001; 142(12): 5172 - 5181.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. Griffin, G. Flouriot, P. Sharp, G. Greene, and F. Gannon
Distribution Analysis of the Two Chicken Estrogen Receptor-Alpha Isoforms and Their Transcripts in the Hypothalamus and Anterior Pituitary Gland
Biol Reprod, October 1, 2001; 65(4): 1156 - 1163.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. T. Jansen, C. West, M. N. Lehman, and V. Padmanabhan
Ovarian Estrogen Receptor-{beta} (ER{beta}) Regulation: I. Changes in ER{beta} Messenger RNA Expression Prior to Ovulation in the Ewe
Biol Reprod, September 1, 2001; 65(3): 866 - 872.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. D. Calder, M. Manikkam, B. E. Salfen, R. S. Youngquist, D. B. Lubahn, W. R. Lamberson, and H. Allen Garverick
Dominant Bovine Ovarian Follicular Cysts Express Increased Levels of Messenger RNAs for Luteinizing Hormone Receptor and 3{beta}-Hydroxysteroid Dehydrogenase {Delta}4,{Delta}5 Isomerase Compared to Normal Dominant Follicles
Biol Reprod, August 1, 2001; 65(2): 471 - 476.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
H. Cardenas, K.A. Burke, R.M. Bigsby, W.F. Pope, and K.P. Nephew
Estrogen Receptor {beta} in the Sheep Ovary During the Estrous Cycle and Early Pregnancy
Biol Reprod, July 1, 2001; 65(1): 128 - 134.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
C. P. Leo, M. D. Pisarska, and A. J.W. Hsueh
DNA Array Analysis of Changes in Preovulatory Gene Expression in the Rat Ovary
Biol Reprod, July 1, 2001; 65(1): 269 - 276.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
S. F. Palter, A. B. Tavares, A. Hourvitz, J. D. Veldhuis, and E. Y. Adashi
Are Estrogens of Import to Primate/Human Ovarian Folliculogenesis?
Endocr. Rev., June 1, 2001; 22(3): 389 - 424.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
C. Guo, L. Savage, K. D. Sarge, and O.-K. Park-Sarge
Gonadotropins Decrease Estrogen Receptor-{beta} Messenger Ribonucleic Acid Stability in Rat Granulosa Cells
Endocrinology, June 1, 2001; 142(6): 2230 - 2237.
[Abstract] [Full Text] [PDF]

Home page
 Exp. Biol. Med.Home page
H. Nejaty, M. Lacey, and S. A. Whitehead
Differing Effects of Endocrine-Disrupting Chemicals on Basal and FSH-Stimulated Progesterone Production in Rat Granulosa-Luteal Cells
Experimental Biology and Medicine, June 1, 2001; 226(6): 570 - 576.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
T. Kurita, K.-j. Lee, P. T.K. Saunders, P. S. Cooke, J. A. Taylor, D. B. Lubahn, C. Zhao, S. Mäkelä, J.-A. Gustafsson, R. Dahiya, et al.
Regulation of Progesterone Receptors and Decidualization in Uterine Stroma of the Estrogen Receptor-{{alpha}} Knockout Mouse
Biol Reprod, January 1, 2001; 64(1): 272 - 283.
[Abstract] [Full Text]

Home page
 J. Clin. Endocrinol. Metab.Home page
G. Pelletier and M. El-Alfy
Immunocytochemical Localization of Estrogen Receptors {{alpha}} and {beta} in the Human Reproductive Organs
J. Clin. Endocrinol. Metab., December 1, 2000; 85(12): 4835 - 4840.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
B. Bao, N. Kumar, R. M. Karp, H. Allen Garverick, and K. Sundaram
Estrogen Receptor-{beta} Expression in Relation to the Expression of Luteinizing Hormone Receptor and Cytochrome P450 Enzymes in Rat Ovarian Follicles
Biol Reprod, December 1, 2000; 63(6): 1747 - 1755.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
H. Wang, H. Eriksson, and L. Sahlin
Estrogen Receptors {alpha} and {beta} in the Female Reproductive Tract of the Rat During the Estrous Cycle
Biol Reprod, November 1, 2000; 63(5): 1331 - 1340.
[Abstract] [Full Text]

Home page
 J. Clin. Endocrinol. Metab.Home page
C.-H. Chiang, K. W. Cheng, S. Igarashi, P. S. Nathwani, and P. C. K. Leung
Hormonal Regulation of Estrogen Receptor {alpha} and {beta} Gene Expression in Human Granulosa-Luteal Cells in Vitro
J. Clin. Endocrinol. Metab., October 1, 2000; 85(10): 3828 - 3839.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
C. Tessier, S. Deb, A. Prigent-Tessier, S. Ferguson-Gottschall, G. B. Gibori, R. P. C. Shiu, and G. Gibori
Estrogen Receptors {alpha} and {beta} in Rat Decidua Cells: Cell-Specific Expression and Differential Regulation by Steroid Hormones and Prolactin
Endocrinology, October 1, 2000; 141(10): 3842 - 3851.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
P. T.K. Saunders, M. R. Millar, K. Williams, S. Macpherson, D. Harkiss, R. A. Anderson, B. Orr, N. P. Groome, G. Scobie, and H. M. Fraser
Differential Expression of Estrogen Receptor-{alpha} and -{beta} and Androgen Receptor in the Ovaries of Marmosets and Humans
Biol Reprod, October 1, 2000; 63(4): 1098 - 1105.
[Abstract] [Full Text]

Home page
 Toxicol PatholHome page
K. Takegawa, K. Mitsumori, K. Yasuhara, M. Moriyasu, M. Sakamori, H. Onodera, M. Hirose, and T. Nomura
A Mechanistic Study of Ovarian Carcinogenesis Induced by Nitrofurazone Using rasH2 Mice
Toxicol Pathol, September 1, 2000; 28(5): 649 - 655.
[Abstract] [PDF]

Home page
 EndocrinologyHome page
D. M. Duffy, C. L. Chaffin, and R. L. Stouffer
Expression of Estrogen Receptor {alpha} and {beta} in the Rhesus Monkey Corpus Luteum during the Menstrual Cycle: Regulation by Luteinizing Hormone and Progesterone
Endocrinology, May 1, 2000; 141(5): 1711 - 1717.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
D. A. Gould, G. J. Moscoso, M. P. A. Young, and D. P. J. Barton
Human First Trimester Fetal Ovaries Express Oncofetal Antigens and Steroid Receptors
Reproductive Sciences, March 1, 2000; 7(2): 131 - 138.
[Abstract] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
S. Chu, P. Mamers, H. G. Burger, and P. J. Fuller
Estrogen Receptor Isoform Gene Expression in Ovarian Stromal and Epithelial Tumors
J. Clin. Endocrinol. Metab., March 1, 2000; 85(3): 1200 - 1205.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
C. S. Rosenfeld, A. A. Murray, G. Simmer, M. G. Hufford, M. F. Smith, N. Spears, and D. B. Lubahn
Gonadotropin Induction of Ovulation and Corpus Luteum Formation in Young Estrogen Receptor-{alpha} Knockout Mice
Biol Reprod, March 1, 2000; 62(3): 599 - 605.
[Abstract] [Full Text]

Home page
 J. Biol. Chem.Home page
B. Saville, M. Wormke, F. Wang, T. Nguyen, E. Enmark, G. Kuiper, J.-A. Gustafsson, and S. Safe
Ligand-, Cell-, and Estrogen Receptor Subtype (alpha /beta )-dependent Activation at GC-rich (Sp1) Promoter Elements
J. Biol. Chem., February 25, 2000; 275(8): 5379 - 5387.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
W. N. Jefferson, J. F. Couse, E. P. Banks, K. S. Korach, and R. R. Newbold
Expression of Estrogen Receptor {beta} Is Developmentally Regulated in Reproductive Tissues of Male and Female Mice
Biol Reprod, February 1, 2000; 62(2): 310 - 317.
[Abstract] [Full Text]

Home page
 DevelopmentHome page
S Dupont, A Krust, A Gansmuller, A Dierich, P Chambon, and M Mark
Effect of single and compound knockouts of estrogen receptors alpha (ERalpha) and beta (ERbeta) on mouse reproductive phenotypes
Development, January 10, 2000; 127(19): 4277 - 4291.
[Abstract] [PDF]

Home page
 Reproductive SciencesHome page
J.- A. Gustafsson
Novel Aspects of Estrogen Action
Reproductive Sciences, January 1, 2000; 7(1_suppl): S8 - S9.
[Abstract] [PDF]

Home page
 EndocrinologyHome page
J. F. Couse, D. O. Bunch, J. Lindzey, D. W. Schomberg, and K. S. Korach
Prevention of the Polycystic Ovarian Phenotype and Characterization of Ovulatory Capacity in the Estrogen Receptor-{alpha} Knockout Mouse
Endocrinology, December 1, 1999; 140(12): 5855 - 5865.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
G. Pelletier, V. Luu-The, A. Charbonneau, and F. Labrie
Cellular Localization of Estrogen Receptor Beta Messenger Ribonucleic Acid in Cynomolgus Monkey Reproductive Organs
Biol Reprod, November 1, 1999; 61(5): 1249 - 1255.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
M. L. O’Brien, K. Park, Y. In, and O.-K. Park-Sarge
Characterization of Estrogen Receptor-{beta} (ER{beta}) Messenger Ribonucleic Acid and Protein Expression in Rat Granulosa Cells
Endocrinology, October 1, 1999; 140(10): 4530 - 4541.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
C. L. Chaffin, R. L. Stouffer, and D. M. Duffy
Gonadotropin and Steroid Regulation of Steroid Receptor and Aryl Hydrocarbon Receptor Messenger Ribonucleic Acid in Macaque Granulosa Cells during the Periovulatory Interval
Endocrinology, October 1, 1999; 140(10): 4753 - 4760.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
S. C. Sharma, J. W. Clemens, M. D. Pisarska, and J. S. Richards
Expression and Function of Estrogen Receptor Subtypes in Granulosa Cells: Regulation by Estradiol and Forskolin
Endocrinology, September 1, 1999; 140(9): 4320 - 4334.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
D. Haynes-Johnson, M.-T. Lai, C. Campen, and S. Palmer
Diverse Effects of Tyrosine Kinase Inhibitors on Follicle-Stimulating Hormone-Stimulated Estradiol and Progesterone Production from Rat Granulosa Cells in Serum-Containing Medium and Serum-Free Medium Containing Epidermal Growth Factor
Biol Reprod, July 1, 1999; 61(1): 147 - 153.
[Abstract] [Full Text]

Home page
 Endocr. Rev.Home page
J. F. Couse and K. S. Korach
Estrogen Receptor Null Mice: What Have We Learned and Where Will They Lead Us?
Endocr. Rev., June 1, 1999; 20(3): 358 - 417.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
J. A. Elvin, C. Yan, P. Wang, K. Nishimori, and M. M. Matzuk
Molecular Characterization of the Follicle Defects in the Growth Differentiation Factor 9-Deficient Ovary
Mol. Endocrinol., June 1, 1999; 13(6): 1018 - 1034.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
S. L. Fitzpatrick, J. M. Funkhouser, D. M. Sindoni, P. E. Stevis, D. C. Deecher, A. R. Bapat, I. Merchenthaler, and D. E. Frail
Expression of Estrogen Receptor-{beta} Protein in Rodent Ovary
Endocrinology, June 1, 1999; 140(6): 2581 - 2591.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
N. A. Mitchner, C. Garlick, R. W. Steinmetz, and N. Ben-Jonathan
Differential Regulation and Action of Estrogen Receptors {alpha} and {beta} in GH3 Cells
Endocrinology, June 1, 1999; 140(6): 2651 - 2658.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
D. W. Schomberg, J. F. Couse, A. Mukherjee, D. B. Lubahn, M. Sar, K. E. Mayo, and K. S. Korach
Targeted Disruption of the Estrogen Receptor-{alpha} Gene in Female Mice: Characterization of Ovarian Responses and Phenotype in the Adult
Endocrinology, June 1, 1999; 140(6): 2733 - 2744.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
Q. Zhang, X. Wang, and D. J. Wolgemuth
Developmentally Regulated Expression of Cyclin D3 and Its Potential in Vivo Interacting Proteins during Murine Gametogenesis
Endocrinology, June 1, 1999; 140(6): 2790 - 2800.
[Abstract] [Full Text]

Home page
 Proc. Natl. Acad. Sci. USAHome page
K.-M. Lau, S. C. Mok, and S.-M. Ho
Expression of human estrogen receptor-alpha and -beta , progesterone receptor, and androgen receptor mRNA in normal and malignant ovarian epithelial cells
PNAS, May 11, 1999; 96(10): 5722 - 5727.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. S. Sperry and P. Thomas
Characterization of Two Nuclear Androgen Receptors in Atlantic Croaker: Comparison of Their Biochemical Properties and Binding Specificities
Endocrinology, April 1, 1999; 140(4): 1602 - 1611.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
C. S. Rosenfeld, X. Yuan, M. Manikkam, M. D. Calder, H. A. Garverick, and D. B. Lubahn
Cloning, Sequencing, and Localization of Bovine Estrogen Receptor-ß within the Ovarian Follicle
Biol Reprod, March 1, 1999; 60(3): 691 - 697.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
M. Sar and F. Welsch
Differential Expression of Estrogen Receptor-{beta} and Estrogen Receptor-{alpha} in the Rat Ovary
Endocrinology, February 1, 1999; 140(2): 963 - 971.
[Abstract] [Full Text]

Home page
 Mol Hum ReprodHome page
R. Misao, Y. Nakanishi, W.S. Sun, J. Fujimoto, S. Iwagaki, R. Hirose, and T. Tamaya
Expression of oestrogen receptor {alpha} and ß mRNA in corpus luteum of human subjects
Mol. Hum. Reprod., January 1, 1999; 5(1): 17 - 21.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
J. H. Krege, J. B. Hodgin, J. F. Couse, E. Enmark, M. Warner, J. F. Mahler, M. Sar, K. S. Korach, J.-A. Gustafsson, and O. Smithies
Generation and reproductive phenotypes of mice lacking estrogen receptor beta
PNAS, December 22, 1998; 95(26): 15677 - 15682.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
G. Flouriot, C. Griffin, M. Kenealy, V. Sonntag-Buck, and F. Gannon
Differentially Expressed Messenger RNA Isoforms of the Human Estrogen Receptor-{alpha} Gene Are Generated by Alternative Splicing and Promoter Usage
Mol. Endocrinol., December 1, 1998; 12(12): 1939 - 1954.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
C. Griffin, G. Flouriot, V. Sonntag-Buck, P. Nestor, and F. Gannon
Identification of Novel Chicken Estrogen Receptor-{alpha} Messenger Ribonucleic Acid Isoforms Generated by Alternative Splicing and Promoter Usage
Endocrinology, November 1, 1998; 139(11): 4614 - 4625.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. A. Mitchner, C. Garlick, and N. Ben-Jonathan
Cellular Distribution and Gene Regulation of Estrogen Receptors {alpha} and {beta} in the Rat Pituitary Gland
Endocrinology, September 1, 1998; 139(9): 3976 - 3983.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. W. Clemens, R. L. Robker, W. L. Kraus, B. S. Katzenellenbogen, and J. S. Richards
Hormone Induction of Progesterone Receptor (PR) Messenger Ribonucleic Acid and Activation of PR Promoter Regions in Ovarian Granulosa Cells: Evidence for a Role of Cyclic Adenosine 3',5'-Monophosphate but Not Estradiol
Mol. Endocrinol., August 1, 1998; 12(8): 1201 - 1214.
[Abstract] [Full Text]

Home page
 Circ. Res.Home page
V. Lindner, S. K. Kim, R. H. Karas, G. G. J. M. Kuiper, J.-A. Gustafsson, and M. E. Mendelsohn
Increased Expression of Estrogen Receptor-ß mRNA in Male Blood Vessels After Vascular Injury
Circ. Res., July 27, 1998; 83(2): 224 - 229.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
V. Kusec, A. S. Virdi, R. Prince, and J. T. Triffitt
Localization of Estrogen Receptor-{alpha} in Human and Rabbit Skeletal Tissues
J. Clin. Endocrinol. Metab., July 1, 1998; 83(7): 2421 - 2428.
[Abstract] [Full Text]

Home page
 Biol. Reprod.Home page
R. L. Robker and J. S. Richards
Hormonal Control of the Cell Cycle in Ovarian Cells: Proliferation Versus Differentiation
Biol Reprod, July 1, 1998; 59(3): 476 - 482.
[Full Text]

Home page
 EndocrinologyHome page
C. M. Telleria, L. Zhong, S. Deb, R. K. Srivastava, K. S. Park, N. Sugino, O.-K. Park-Sarge, and G. Gibori
Differential Expression of the Estrogen Receptors {alpha} and {beta} in the Rat Corpus Luteum of Pregnancy: Regulation by Prolactin and Placental Lactogens
Endocrinology, May 1, 1998; 139(5): 2432 - 2442.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
N. Sugino, M. Zilberstein, R. K. Srivastava, C. M. Telleria, S. E. Nelson, M. Risk, J. Y. Chou, and G. Gibori
Establishment and Characterization of a Simian Virus 40-Transformed Temperature-Sensitive Rat Luteal Cell Line
Endocrinology, April 1, 1998; 139(4): 1936 - 1942.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
R. A. K. Srivastava, N. Srivastava, M. Averna, R. C. Lin, K. S. Korach, D. B. Lubahn, and G. Schonfeld
Estrogen Up-regulates Apolipoprotein E (ApoE) Gene Expression by Increasing ApoE mRNA in the Translating Pool via the Estrogen Receptor alpha -Mediated Pathway
J. Biol. Chem., December 26, 1997; 272(52): 33360 - 33366.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S. K. Das, J. A. Taylor, K. S. Korach, B. C. Paria, S. K. Dey, and D. B. Lubahn
Estrogenic responses in estrogen receptor-alpha deficient mice reveal a distinct estrogen signaling pathway
PNAS, November 25, 1997; 94(24): 12786 - 12791.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
J. F. Couse, J. Lindzey, K. Grandien, J.-A. Gustafsson, and K. S. Korach
Tissue Distribution and Quantitative Analysis of Estrogen Receptor-{alpha} (ER{alpha}) and Estrogen Receptor-{beta} (ER{beta}) Messenger Ribonucleic Acid in the Wild-Type and ER{alpha}-Knockout Mouse
Endocrinology, November 1, 1997; 138(11): 4613 - 4621.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
K. Pettersson, K. Grandien, G. G. J. M. Kuiper, and J.-A. Gustafsson
Mouse Estrogen Receptor {beta} Forms Estrogen Response Element-Binding Heterodimers with Estrogen Receptor {alpha}
Mol. Endocrinol., September 1, 1997; 11(10): 1486 - 1496.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1997 by The Endocrine Society