| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Division of Reproductive Biology (M.C.), Department of
Gynecology and Obstetrics, Stanford University Medical Center,
Stanford, California 94305,
Institute of Histology and General
Embriology (E.V.), School of Medicine, University La Sapienza,
00161 Rome, Italy
In the Sertoli cell, FSH stimulates transcription of a cAMP-phosphodiesterase (PDE) gene (PDE4D) and accumulation of corresponding mRNA and PDE protein. The regulation of this PDE gene is an important component of the desensitization state induced by this hormone. Given the ubiquitous nature of this regulation controlling cAMP levels, the molecular basis for the PDE4D induction was further investigated. FSH stimulation of the Sertoli cell causes the accumulation of only two of the four known PDE4D mRNAs (PDE4D1 and PDE4D2). The promoter controlling the expression of these two messages was identified and characterized. An EcoRI fragment containing a coding exon as well as 5'-upstream sequence of the PDE4D1/2 mRNA was isolated from rat genomic libraries and sequenced. No TATA box was identified, but GC-rich regions were present upstream of the putative translation start site. RNAse protection and PCR analysis indicated the presence of at least two distinct cap sites. This genomic region had promoter activity when transfected both in Sertoli and MA-10 cells. Deletion mutation indicated that basal promoter activity was contributed by regions upstream of both cap sites. Transcription from this promoter was activated by FSH and (Bu)2cAMP, and elements responsible for cAMP regulation were present upstream from the second cap site. These data demonstrate that an intronic promoter that is cAMP- and hormone-inducible directs the expression of these truncated PDE proteins.
This article has been cited by other articles:
 |
|
 |
|
  M. P. Kelly, Y.-F. Cheung, C. Favilla, S. J. Siegel, S. J. Kanes, M. D. Houslay, and T. Abel Constitutive activation of the G-protein subunit G{alpha}s within forebrain neurons causes PKA-dependent alterations in fear conditioning and cortical Arc mRNA expression Learn. Mem., January 28, 2008; 15(2): 75 - 83. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. C. Kostek, Y.-W. Chen, D. J. Cuthbertson, R. Shi, M. J. Fedele, K. A. Esser, and M. J. Rennie Gene expression responses over 24 h to lengthening and shortening contractions in human muscle: major changes in CSRP3, MUSTN1, SIX1, and FBXO32 Physiol Genomics, September 11, 2007; 31(1): 42 - 52. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Levallet, J. Levallet, H. Bouraima-Lelong, and P.-J. Bonnamy Expression of the cAMP-Phosphodiesterase PDE4D Isoforms and Age-Related Changes in Follicle-Stimulating Hormone-Stimulated PDE4 Activities in Immature Rat Sertoli Cells Biol Reprod, May 1, 2007; 76(5): 794 - 803. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. D. Houslay, G. S. Baillie, and D. H. Maurice cAMP-Specific Phosphodiesterase-4 Enzymes in the Cardiovascular System: A Molecular Toolbox for Generating Compartmentalized cAMP Signaling Circ. Res., April 13, 2007; 100(7): 950 - 966. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
K. Omori and J. Kotera Overview of PDEs and Their Regulation Circ. Res., February 16, 2007; 100(3): 309 - 327. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 D. G. Tilley and D. H. Maurice Vascular Smooth Muscle Cell Phenotype-Dependent Phosphodiesterase 4D Short Form Expression: Role of Differential Histone Acetylation on cAMP-Regulated Function Mol. Pharmacol., September 1, 2005; 68(3): 596 - 605. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. D. Houslay The Long and Short of Vascular Smooth Muscle Phosphodiesterase-4 As a Putative Therapeutic Target Mol. Pharmacol., September 1, 2005; 68(3): 563 - 567. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
D. A. Wallace, L. A. Johnston, E. Huston, D. MacMaster, T. M. Houslay, Y.-F. Cheung, L. Campbell, J. E. Millen, R. A. Smith, I. Gall, et al. Identification and Characterization of PDE4A11, a Novel, Widely Expressed Long Isoform Encoded by the Human PDE4A cAMP Phosphodiesterase Gene Mol. Pharmacol., June 1, 2005; 67(6): 1920 - 1934. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. Euskirchen, T. E. Royce, P. Bertone, R. Martone, J. L. Rinn, F. K. Nelson, F. Sayward, N. M. Luscombe, P. Miller, M. Gerstein, et al. CREB Binds to Multiple Loci on Human Chromosome 22 Mol. Cell. Biol., May 1, 2004; 24(9): 3804 - 3814. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 J.-Y. Park, F. Richard, S.-Y. Chun, J.-H. Park, E. Law, K. Horner, S-L C. Jin, and M. Conti Phosphodiesterase Regulation Is Critical for the Differentiation and Pattern of Gene Expression in Granulosa Cells of the Ovarian Follicle Mol. Endocrinol., June 1, 2003; 17(6): 1117 - 1130. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Conti, W. Richter, C. Mehats, G. Livera, J.-Y. Park, and C. Jin Cyclic AMP-specific PDE4 Phosphodiesterases as Critical Components of Cyclic AMP Signaling J. Biol. Chem., February 14, 2003; 278(8): 5493 - 5496. [Full Text] [PDF]
|
|
|
|
|
|
I. R. Le Jeune, M. Shepherd, G. Van Heeke, M. D. Houslay, and I. P. Hall Cyclic AMP-dependent Transcriptional Up-regulation of Phosphodiesterase 4D5 in Human Airway Smooth Muscle Cells. IDENTIFICATION AND CHARACTERIZATION OF A NOVEL PDE4D5 PROMOTER J. Biol. Chem., September 20, 2002; 277(39): 35980 - 35989. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 C. Mehats, G. Tanguy, E. Dallot, D. Cabrol, F. Ferre, and M.-J. Leroy Is Up-Regulation of Phosphodiesterase 4 Activity by PGE2 Involved in the Desensitization of {beta}-Mimetics in Late Pregnancy Human Myometrium? J. Clin. Endocrinol. Metab., November 1, 2001; 86(11): 5358 - 5365. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. Persani, S. Borgato, A. Lania, M. Filopanti, G. Mantovani, M. Conti, and A. Spada Relevant cAMP-Specific Phosphodiesterase Isoforms in Human Pituitary: Effect of Gs{alpha} Mutations J. Clin. Endocrinol. Metab., August 1, 2001; 86(8): 3795 - 3800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. Rena, F. Begg, A. Ross, C. MacKenzie, I. McPhee, L. Campbell, E. Huston, M. Sullivan, and M. D. Houslay Molecular Cloning, Genomic Positioning, Promoter Identification, and Characterization of the Novel Cyclic AMP-Specific Phosphodiesterase PDE4A10 Mol. Pharmacol., April 16, 2001; 59(5): 996 - 1011. [Abstract] [Full Text]
|
|
|
|
|
|
L. Persani, A. Lania, L. Alberti, R. Romoli, G. Mantovani, S. Filetti, A. Spada, and M. Conti Induction of Specific Phosphodiesterase Isoforms by Constitutive Activation of the cAMP Pathway in Autonomous Thyroid Adenomas J. Clin. Endocrinol. Metab., August 1, 2000; 85(8): 2872 - 2878. [Abstract] [Full Text]
|
|
|
|
 |
|
 F. Naro, C. Sette, E. Vicini, V. De Arcangelis, M. Grange, M. Conti, M. Lagarde, M. Molinaro, S. Adamo, and G. Némoz Involvement of Type 4 cAMP-Phosphodiesterase in the Myogenic Differentiation of L6 Cells Mol. Biol. Cell, December 1, 1999; 10(12): 4355 - 4367. [Abstract] [Full Text]
|
|
|
|
 |
|
 C. Méhats, G. Tanguy, E. Dallot, B. Robert, R. Rebourcet, F. Ferré, and M. J. Leroy Selective Up-Regulation of Phosphodiesterase-4 Cyclic Adenosine 3',5'-Monophosphate (cAMP)-Specific Phosphodiesterase Variants by Elevated cAMP Content in Human Myometrial Cells in Culture Endocrinology, July 1, 1999; 140(7): 3228 - 3237. [Abstract] [Full Text]
|
|
|
|
|
|
M. Salanova, S.-Y. Chun, S. Iona, C. Puri, M. Stefanini, and M. Conti Type 4 Cyclic Adenosine Monophosphate-Specific Phosphodiesterases Are Expressed in Discrete Subcellular Compartments during Rat Spermiogenesis Endocrinology, May 1, 1999; 140(5): 2297 - 2306. [Abstract] [Full Text]
|
|
|
|
|
|
L. M. Grønning, M. K. Dahle, K. A. Taskén, S. Enerbäck, L. Hedin, K. Taskén, and H. K. Knutsen Isoform-Specific Regulation of the CCAAT/Enhancer-Binding Protein Family of Transcription Factors by 3',5'-Cyclic Adenosine Monophosphate in Sertoli Cells Endocrinology, February 1, 1999; 140(2): 835 - 843. [Abstract] [Full Text]
|
|
|
|
|
|
S.-L. C. Jin, T. Bushnik, L. Lan, and M. Conti Subcellular Localization of Rolipram-sensitive, cAMP-specific Phosphodiesterases. DIFFERENTIAL TARGETING AND ACTIVATION OF THE SPLICING VARIANTS DERIVED FROM THE PDE4D GENE J. Biol. Chem., July 31, 1998; 273(31): 19672 - 19678. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 T. J. TORPHY Phosphodiesterase Isozymes . Molecular Targets for Novel Antiasthma Agents Am. J. Respir. Crit. Care Med., February 1, 1997; 157(2): 351 - 370. [Full Text]
|
|
|
|
|
|
K. A. Tasken, P. Collas, W. A. Kemmner, O. Witczak, M. Conti, and K. Tasken Phosphodiesterase 4D and Protein Kinase A Type II Constitute a Signaling Unit in the Centrosomal Area J. Biol. Chem., June 15, 2001; 276(25): 21999 - 22002. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Liu, D. Palmer, S. L. Jimmo, D. G. Tilley, H. A. Dunkerley, S. C. Pang, and D. H. Maurice Expression of Phosphodiesterase 4D (PDE4D) Is Regulated by Both the Cyclic AMP-dependent Protein Kinase and Mitogen-activated Protein Kinase Signaling Pathways. A POTENTIAL MECHANISM ALLOWING FOR THE COORDINATED REGULATION OF PDE4D ACTIVITY AND EXPRESSION IN CELLS J. Biol. Chem., August 18, 2000; 275(34): 26615 - 26624. [Abstract] [Full Text] [PDF]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
