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Intracellular Trafficking of Angiotensin II and its AT₁ and AT₂ Receptors: Evidence for Selective Sorting of Receptor and Ligand

Falk Cardiovascular Research Center and Department of Medicine (L.H., R.E.P., V.J.D., B.K.K.) Howard Hughes Medical Institute (B.K.K.) Stanford University School of Medicine Stanford, California 94305
Department of Pharmacology (L.H., L.M.) University of Wuerzburg Wuerzburg, Germany
Department of Medicine (R.E.P, V.J.D.) Brigham and Women’s Hospital Harvard Medical School Boston, Massachusetts 02115

Angiotensin II (Ang II) binds to two different receptor subtypes, AT₁ and AT₂ receptors. In many cases, receptor stimulation by Ang II is followed by a rapid desensitization of the intracellular signal transduction and a decrease in cell surface receptor number. The present study was designed to examine by immunofluorescence microscopy the cellular trafficking pathways of Ang II and its AT_1a and AT₂ receptors in human embryonal kidney 293 cells stably expressing these receptor subtypes. Fluorescently labeled Ang II and AT_1a receptors were rapidly internalized into endosomes. AT₂ receptors were localized in the plasma membrane and did not undergo endocytosis upon agonist stimulation. After removal of agonist, AT_1a receptors recycled to the plasma membrane, whereas fluorescently labeled Ang II was targeted to the lysosomal pathway. Even though no further loss of surface receptor was measurable by ligand binding at steady state, fluorescein-Ang II was continuously internalized, and cycling of receptor between endosomal vesicles and the plasma membrane was detected by antibody feeding. These experiments provide evidence for subtype-specific receptor sorting and internalization of Ang II and its AT_1a receptor as a receptor-ligand complex, and they suggest that the sequestration of receptors into endosomes is in dynamic equilibrium with receptor cycling to the plasma membrane. Finally, internalization of AT_1a receptors and Ang II persists after desensitization mechanisms have attenuated Ca²⁺ and inositol 1,4,5-trisphosphate signaling.

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