This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Tsai-Pflugfelder, M. Articles by Wahli, W. Search for Related Content PubMed PubMed Citation Articles by Tsai-Pflugfelder, M. Articles by Wahli, W.

Functional Interaction between the Estrogen Receptor and CTF1: Analysis of the Vitellogenin Gene B1 Promoter in Yeast

Institut de Biologie animale (M.T.-P., W.W.) Université de Lausanne Bâtiment de Biologie CH-1015 Lausanne, Switzerland
Swiss Institute for Experimental Cancer Research (M.T.-P., S.M.G.) CH-1066 Epalinges, Switzerland

Eukaryotic gene expression depends on a complex interplay between the transcriptional apparatus and chromatin structure. We report here a yeast model system for investigating the functional interaction between the human estrogen receptor (hER) and CTF1, a member of the CTF/NFI transcription factor family. We show that a CTF1-fusion protein and the hER transactivate a synthetic promoter in yeast in a synergistic manner. This interaction requires the proline-rich transactivation domain of CTF1. When the natural estrogen-dependent vitellogenin B1 promoter is tested in yeast, CTF1 and CTF1-fusion proteins are unable to activate transcription, and no synergy is observed between hER, which activates the B1 promoter, and these factors. Chromatin structure analysis on this promoter reveals positioned nucleosomes at -430 to -270 (±20 bp) and at -270 to -100 (±20 bp) relative to the start site of transcription. The positions of the nucleosomes remain unchanged upon hormone-dependent transcriptional activation of the promoter, and the more proximal nucleosome appears to mask the CTF/NFI site located at -101 to -114. We conclude that a functional interaction of hER with the estrogen response element located upstream of a basal promoter occurs in yeast despite the nucleosomal organization of this promoter, whereas the interaction of CTF1 with its target site is apparently precluded by a nucleosome.

This article has been cited by other articles:

				D. Robyr, A. Gegonne, A. P. Wolffe, and W. Wahli Determinants of Vitellogenin B1 Promoter Architecture. HNF3 AND ESTROGEN RESPONSIVE TRANSCRIPTION WITHIN CHROMATIN J. Biol. Chem., September 1, 2000; 275(36): 28291 - 28300. [Abstract] [Full Text] [PDF]