help button home button Endocrine Society Molecular Endocrinology ENDO 08 Sessions Library
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Kostich, W. A.
Right arrow Articles by Largent, B. L.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Kostich, W. A.
Right arrow Articles by Largent, B. L.
Molecular Endocrinology 12 (8): 1077-1085
Copyright © 1998 by The Endocrine Society

Molecular Identification and Analysis of a Novel Human Corticotropin-Releasing Factor (CRF) Receptor: The CRF2{gamma} Receptor

Walter A. Kostich1, Airu Chen1, Karen Sperle and Brian L. Largent

DuPont Merck Research Laboratories CNS Diseases Research Wilmington, Delaware 19880

We report the discovery of a new CRF2 receptor splice isoform found in human brain, which we have termed the CRF2{gamma} receptor. The CRF2{gamma} cDNA encodes for a 397-amino acid receptor that has an amino terminus with no significant homology to the already reported {alpha}- and ß-termini. When expressed in 293-EBNA (Epstein-Barr nuclear antigen) cells, the CRF2{gamma} receptor responds in a dose-dependent manner to CRF and related peptides with a rank order of potency of urocortin>=sauvagine>urotensin>r/h CRF, with EC50 values more similar to CRF2{alpha} than CRF2ß. Equilibrium saturation isotherm analysis with radiolabeled sauvagine reveals a two site/state model for binding to CRF2{gamma} with a 60 pM Kd high-affinity site and a 5 nM Kd low-affinity site. Analysis of CRF2{gamma} RNA expression in human brain demonstrates expression in septum and hippocampus, with weaker but detectable expression in amygdala, nucleus accumbens, midbrain, and frontal cortex.




This article has been cited by other articles:


Home page
 FASEB J.Home page
Y. Sztainberg, Y. Kuperman, O. Issler, S. Gil, J. Vaughan, J. Rivier, W. Vale, and A. Chen
A novel corticotropin-releasing factor receptor splice variant exhibits dominant negative activity: a putative link to stress-induced heart disease
FASEB J, July 1, 2009; 23(7): 2186 - 2196.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Pharmacol.Home page
E. Gutknecht, I. Van der Linden, K. Van Kolen, K. F. C. Verhoeven, G. Vauquelin, and F. M. Dautzenberg
Molecular Mechanisms of Corticotropin-Releasing Factor Receptor-Induced Calcium Signaling
Mol. Pharmacol., March 1, 2009; 75(3): 648 - 657.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Gao, C. Lu, C. Xu, Y. Tao, B. Cong, and X. Ni
Differential Regulation of Prostaglandin Production Mediated by Corticotropin-Releasing Hormone Receptor Type 1 and Type 2 in Cultured Human Placental Trophoblasts
Endocrinology, June 1, 2008; 149(6): 2866 - 2876.
[Abstract] [Full Text] [PDF]

Home page
 Reproductive SciencesHome page
Dan Jin, Ping He, Xingji You, Xiaoyan Zhu, Ling Dai, Qian He, Chunmin Liu, Ning Hui, Jinyan Sha, and Xin Ni
Expression of Corticotropin-Releasing Hormone Receptor Type 1 and Type 2 in Human Pregnant Myometrium
Reproductive Sciences, September 1, 2007; 14(6): 568 - 577.
[Abstract] [PDF]

Home page
 GutHome page
A. C Moss, P. Anton, T. Savidge, P. Newman, A. S Cheifetz, J. Gay, S. Paraschos, M. W. Winter, M. P Moyer, K. Karalis, et al.
Urocortin II mediates pro-inflammatory effects in human colonocytes via corticotropin-releasing hormone receptor 2{alpha}
Gut, September 1, 2007; 56(9): 1210 - 1217.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
L. Gao, P. He, J. Sha, C. Liu, L. Dai, N. Hui, and X. Ni
Corticotropin-Releasing Hormone Receptor Type 1 and Type 2 Mediate Differential Effects on 15-Hydroxy Prostaglandin Dehydrogenase Expression in Cultured Human Chorion Trophoblasts
Endocrinology, August 1, 2007; 148(8): 3645 - 3654.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
S. V. Wu, P.-q. Yuan, L. Wang, Y. L. Peng, C.-Y. Chen, and Y. Tache
Identification and Characterization of Multiple Corticotropin-Releasing Factor Type 2 Receptor Isoforms in the Rat Esophagus
Endocrinology, April 1, 2007; 148(4): 1675 - 1687.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
T. Nemoto, A. Iwasaki-Sekino, N. Yamauchi, and T. Shibasaki
Regulation of the expression and secretion of urocortin 2 in rat pituitary
J. Endocrinol., February 1, 2007; 192(2): 443 - 452.
[Abstract] [Full Text] [PDF]

Home page
 Eur J EndocrinolHome page
E Zoumakis, D K Grammatopoulos, and G P Chrousos
Corticotropin-releasing hormone receptor antagonists
Eur. J. Endocrinol., November 1, 2006; 155(suppl_1): S85 - S91.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. Kageyama, K. Hanada, T. Nigawara, T. Moriyama, K. Terui, S. Sakihara, and T. Suda
Urocortin Induces Interleukin-6 Gene Expression via Cyclooxygenase-2 Activity in Aortic Smooth Muscle Cells
Endocrinology, September 1, 2006; 147(9): 4454 - 4462.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
E. Kokkotou, D. Torres, A. C. Moss, M. O'Brien, D. E. Grigoriadis, K. Karalis, and C. Pothoulakis
Corticotropin-releasing hormone receptor 2-deficient mice have reduced intestinal inflammatory responses.
J. Immunol., September 1, 2006; 177(5): 3355 - 3361.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
E. W. Hillhouse and D. K. Grammatopoulos
The Molecular Mechanisms Underlying the Regulation of the Biological Activity of Corticotropin-Releasing Hormone Receptors: Implications for Physiology and Pathophysiology
Endocr. Rev., May 1, 2006; 27(3): 260 - 286.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
J. Xu, J. D. Hennebold, and R. L. Stouffer
Dynamic Expression and Regulation of the Corticotropin-Releasing Hormone/Urocortin-Receptor-Binding Protein System in the Primate Ovary during the Menstrual Cycle
J. Clin. Endocrinol. Metab., April 1, 2006; 91(4): 1544 - 1553.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
R. Sirianni, B. A. Mayhew, B. R. Carr, C. R. Parker Jr., and W. E. Rainey
Corticotropin-Releasing Hormone (CRH) and Urocortin Act through Type 1 CRH Receptors to Stimulate Dehydroepiandrosterone Sulfate Production in Human Fetal Adrenal Cells
J. Clin. Endocrinol. Metab., September 1, 2005; 90(9): 5393 - 5400.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
T. Fukuda, K. Takahashi, T. Suzuki, M. Saruta, M. Watanabe, T. Nakata, and H. Sasano
Urocortin 1, Urocortin 3/Stresscopin, and Corticotropin-Releasing Factor Receptors in Human Adrenal and Its Disorders
J. Clin. Endocrinol. Metab., August 1, 2005; 90(8): 4671 - 4678.
[Abstract] [Full Text] [PDF]

Home page
 J. Immunol.Home page
J. Cao, N. Papadopoulou, D. Kempuraj, W. S. Boucher, K. Sugimoto, C. L. Cetrulo, and T. C. Theoharides
Human Mast Cells Express Corticotropin-Releasing Hormone (CRH) Receptors and CRH Leads to Selective Secretion of Vascular Endothelial Growth Factor
J. Immunol., June 15, 2005; 174(12): 7665 - 7675.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
A. M. Chen, M. H. Perrin, M. R. DiGruccio, J. M. Vaughan, B. K. Brar, C. M. Arias, K. A. Lewis, J. E. Rivier, P. E. Sawchenko, and W. W. Vale
A soluble mouse brain splice variant of type 2{alpha} corticotropin-releasing factor (CRF) receptor binds ligands and modulates their activity
PNAS, February 15, 2005; 102(7): 2620 - 2625.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. Chen, M. Perrin, B. Brar, C. Li, P. Jamieson, M. DiGruccio, K. Lewis, and W. Vale
Mouse Corticotropin-Releasing Factor Receptor Type 2{alpha} Gene: Isolation, Distribution, Pharmacological Characterization and Regulation by Stress and Glucocorticoids
Mol. Endocrinol., February 1, 2005; 19(2): 441 - 458.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
M. Saruta, K. Takahashi, T. Suzuki, A. Torii, M. Kawakami, and H. Sasano
Urocortin 1 in Colonic Mucosa in Patients with Ulcerative Colitis
J. Clin. Endocrinol. Metab., November 1, 2004; 89(11): 5352 - 5361.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
A. Slominski, A. Pisarchik, D. J. Tobin, J. E. Mazurkiewicz, and J. Wortsman
Differential Expression of a Cutaneous Corticotropin-Releasing Hormone System
Endocrinology, February 1, 2004; 145(2): 941 - 950.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
K. Kageyama and T. Suda
Urocortin-Related Peptides Increase Interleukin-6 Output via Cyclic Adenosine 5'-Monophosphate-Dependent Pathways in A7r5 Aortic Smooth Muscle Cells
Endocrinology, June 1, 2003; 144(6): 2234 - 2241.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
G. Zhang, N. Huang, Y.-W. Li, X. Qi, A. P. Marshall, X.-X. Yan, G. Hill, C. Rominger, S. R. Prakash, R. Bakthavatchalam, et al.
Pharmacological Characterization of a Novel Nonpeptide Antagonist Radioligand, ({+/-})-N-[2-Methyl-4-methoxyphenyl]-1-(1-(methoxymethyl) propyl)-6-methyl-1H-1,2,3-triazolo[4,5-c]pyridin-4-amine ([3H]SN003) for Corticotropin-Releasing Factor1 Receptors
J. Pharmacol. Exp. Ther., April 1, 2003; 305(1): 57 - 69.
[Abstract] [Full Text]

Home page
 Pharmacol. Rev.Home page
R. L. Hauger, D. E. Grigoriadis, M. F. Dallman, P. M. Plotsky, W. W. Vale, and F. M. Dautzenberg
International Union of Pharmacology. XXXVI. Current Status of the Nomenclature for Receptors for Corticotropin-Releasing Factor and Their Ligands
Pharmacol. Rev., March 1, 2003; 55(1): 21 - 26.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
R. D. Catalano, T. Kyriakou, J. Chen, A. Easton, and E. W. Hillhouse
Regulation of Corticotropin-Releasing Hormone Type 2 Receptors by Multiple Promoters and Alternative Splicing: Identification of Multiple Splice Variants
Mol. Endocrinol., March 1, 2003; 17(3): 395 - 410.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Endocrinol. Metab.Home page
E. Karteris, H. S. Randeva, D. K. Grammatopoulos, R. B. Jaffe, and E. W. Hillhouse
Expression and Coupling Characteristics of the CRH and Orexin Type 2 Receptors in Human Fetal Adrenals
J. Clin. Endocrinol. Metab., September 1, 2001; 86(9): 4512 - 4519.
[Abstract] [Full Text] [PDF]

Home page
 FASEB J.Home page
A. SLOMINSKI, J. WORTSMAN, A. PISARCHIK, B. ZBYTEK, E. A. LINTON, J. E. MAZURKIEWICZ, and E. T. WEI
Cutaneous expression of corticotropin-releasing hormone (CRH), urocortin, and CRH receptors
FASEB J, August 1, 2001; 15(10): 1678 - 1693.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
Z. Sarnyai, Y. Shaham, and S. C. Heinrichs
The Role of Corticotropin-Releasing Factor in Drug Addiction
Pharmacol. Rev., June 1, 2001; 53(2): 209 - 244.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
D. K. Grammatopoulos, H. S. Randeva, M. A. Levine, E. S. Katsanou, and E. W. Hillhouse
Urocortin, but Not Corticotropin-Releasing Hormone (CRH), Activates the Mitogen-Activated Protein Kinase Signal Transduction Pathway in Human Pregnant Myometrium: An Effect Mediated via R1{{alpha}} and R2{beta} CRH Receptor Subtypes and Stimulation of Gq-Proteins
Mol. Endocrinol., December 1, 2000; 14(12): 2076 - 2091.
[Abstract] [Full Text]

Home page
 EndocrinologyHome page
K. Kageyama, G. E. Gaudriault, M. J. Bradbury, and W. W. Vale
Regulation of Corticotropin-Releasing Factor Receptor Type 2{beta} Messenger Ribonucleic Acid in the Rat Cardiovascular System by Urocortin, Glucocorticoids, and Cytokines
Endocrinology, July 1, 2000; 141(7): 2285 - 2293.
[Abstract] [Full Text] [PDF]

Home page
 Physiol. Rev.Home page
A. Slominski, J. Wortsman, T. Luger, R. Paus, and S. Solomon
Corticotropin Releasing Hormone and Proopiomelanocortin Involvement in the Cutaneous Response to Stress
Physiol Rev, July 1, 2000; 80(3): 979 - 1020.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. A. Pelleymounter, M. Joppa, M. Carmouche, M. J. Cullen, B. Brown, B. Murphy, D. E. Grigoriadis, N. Ling, and A. C. Foster
Role of Corticotropin-Releasing Factor (CRF) Receptors in the Anorexic Syndrome Induced by CRF
J. Pharmacol. Exp. Ther., June 1, 2000; 293(3): 799 - 806.
[Abstract] [Full Text]

Home page
 Mol. Endocrinol.Home page
D. K. Grammatopoulos, Y. Dai, H. S. Randeva, M. A. Levine, E. Karteris, A. J. Easton, and E. W. Hillhouse
A Novel Spliced Variant of the Type 1 Corticotropin-Releasing Hormone Receptor with a Deletion in the Seventh Transmembrane Domain Present in the Human Pregnant Term Myometrium and Fetal Membranes
Mol. Endocrinol., December 1, 1999; 13(12): 2189 - 2202.
[Abstract] [Full Text]

Home page
 Am. J. Physiol. Gastrointest. Liver Physiol.Home page
T. Nozu, V. Martinez, J. Rivier, and Y. Tache
Peripheral urocortin delays gastric emptying: role of CRF receptor 2
Am J Physiol Gastrointest Liver Physiol, April 1, 1999; 276(4): G867 - G874.
[Abstract] [Full Text] [PDF]

Home page
 J. Pharmacol. Exp. Ther.Home page
M. H. Perrin, S. W. Sutton, L. A. Cervini, J. E. Rivier, and W. W. Vale
Comparison of an Agonist, Urocortin, and an Antagonist, Astressin, as Radioligands for Characterization of Corticotropin-Releasing Factor Receptors
J. Pharmacol. Exp. Ther., February 1, 1999; 288(2): 729 - 734.
[Abstract] [Full Text]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1998 by The Endocrine Society