This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lee, S.-K. Articles by Lee, J. W. Search for Related Content PubMed PubMed Citation Articles by Lee, S.-K. Articles by Lee, J. W.

Steroid Receptor Coactivator-1 and Its Family Members Differentially Regulate Transactivation by the Tumor Suppressor Protein p53

Center for Ligand and Transcription (J.W.L, H.-J.K) Hormone Research Center (J.W.L) Department of Biology (S.-K.L) Chonnam National University Kwangju 500–757, Korea
Department of Biochemistry (J.W.K) Paichai University Daejeon 302–735, Korea

The tumor suppressor protein p53 exerts its cell cycle-regulatory effects through its ability to function as a sequence-specific DNA-binding transcription factor. Herein, we show that p53 physically interacts with specific subregions of steroid receptor coactivator-1 (SRC-1) and its family members, p/CIP (p300/CBP interacting protein), xSRC-3, and AIB1 (amplified in breast cancer), originally isolated as transcription coactivators of nuclear receptors, as demonstrated by the yeast and mammalian two-hybrid tests as well as glutathione S-transferase pull-down assays. Interestingly, cotransfection of HeLa cells with SRC-1- or p/CIP expression vector potentiated the p53-mediated transactivation, whereas AIB1 and xSRC-3 were repressive. All of these SRC-1 members, however, similarly stimulated transactivation mediated by nuclear receptors and AP-1, as previously described. These results suggest that SRC-1 and its family members may differentially modulate the p53 transactivation in vivo.

This article has been cited by other articles:

				J. Lee, D.-H. Kim, S. Lee, Q.-H. Yang, D. K. Lee, S.-K. Lee, R. G. Roeder, and J. W. Lee A tumor suppressive coactivator complex of p53 containing ASC-2 and histone H3-lysine-4 methyltransferase MLL3 or its paralogue MLL4 PNAS, May 26, 2009; 106(21): 8513 - 8518. [Abstract] [Full Text] [PDF]

				S. Lachize, E. M. Apostolakis, S. van der Laan, A. M. I. Tijssen, J. Xu, E. R. de Kloet, and O. C. Meijer Steroid receptor coactivator-1 is necessary for regulation of corticotropin-releasing hormone by chronic stress and glucocorticoids PNAS, May 12, 2009; 106(19): 8038 - 8042. [Abstract] [Full Text] [PDF]

				A. C-K. Chung, S. Zhou, L. Liao, J. C.-Y. Tien, N. M. Greenberg, and J. Xu Genetic Ablation of the Amplified-in-Breast Cancer 1 Inhibits Spontaneous Prostate Cancer Progression in Mice Cancer Res., June 15, 2007; 67(12): 5965 - 5975. [Abstract] [Full Text] [PDF]

				V. Duong, N. Boulle, S. Daujat, J. Chauvet, S. Bonnet, H. Neel, and V. Cavailles Differential Regulation of Estrogen Receptor {alpha} Turnover and Transactivation by Mdm2 and Stress-Inducing Agents Cancer Res., June 1, 2007; 67(11): 5513 - 5521. [Abstract] [Full Text] [PDF]

				S. Cascio, V. Bartella, C. Garofalo, A. Russo, A. Giordano, and E. Surmacz Insulin-like Growth Factor 1 Differentially Regulates Estrogen Receptor-dependent Transcription at Estrogen Response Element and AP-1 Sites in Breast Cancer Cells J. Biol. Chem., February 9, 2007; 282(6): 3498 - 3506. [Abstract] [Full Text] [PDF]

				H. Naeem, D. Cheng, Q. Zhao, C. Underhill, M. Tini, M. T. Bedford, and J. Torchia The Activity and Stability of the Transcriptional Coactivator p/CIP/SRC-3 Are Regulated by CARM1-Dependent Methylation Mol. Cell. Biol., January 1, 2007; 27(1): 120 - 134. [Abstract] [Full Text] [PDF]

				P. Mussi, C. Yu, B. W. O'Malley, and J. Xu Stimulation of Steroid Receptor Coactivator-3 (SRC-3) Gene Overexpression by a Positive Regulatory Loop of E2F1 and SRC-3 Mol. Endocrinol., December 1, 2006; 20(12): 3105 - 3119. [Abstract] [Full Text] [PDF]

				G. Li, J. H. Heaton, and T. D. Gelehrter Role of Steroid Receptor Coactivators in Glucocorticoid and Transforming Growth Factor {beta} Regulation of Plasminogen Activator Inhibitor Gene Expression Mol. Endocrinol., May 1, 2006; 20(5): 1025 - 1034. [Abstract] [Full Text] [PDF]

				P. Yi, R.-C. Wu, J. Sandquist, J. Wong, S. Y. Tsai, M.-J. Tsai, A. R. Means, and B. W. O'Malley Peptidyl-Prolyl Isomerase 1 (Pin1) Serves as a Coactivator of Steroid Receptor by Regulating the Activity of Phosphorylated Steroid Receptor Coactivator 3 (SRC-3/AIB1) Mol. Cell. Biol., November 1, 2005; 25(21): 9687 - 9699. [Abstract] [Full Text] [PDF]

				R.-C. Wu, C. L. Smith, and B. W. O'Malley Transcriptional Regulation by Steroid Receptor Coactivator Phosphorylation Endocr. Rev., May 1, 2005; 26(3): 393 - 399. [Abstract] [Full Text] [PDF]

				E. Myers, A. D.K. Hill, G. Kelly, E. W. McDermott, N. J. O'Higgins, Y. Buggy, and L. S. Young Associations and Interactions between Ets-1 and Ets-2 and Coregulatory Proteins, SRC-1, AIB1, and NCoR in Breast Cancer Clin. Cancer Res., March 15, 2005; 11(6): 2111 - 2122. [Abstract] [Full Text] [PDF]

				B. Burwinkel, M. Wirtenberger, R. Klaes, R. K. Schmutzler, E. Grzybowska, A. Forsti, B. Frank, J. L. Bermejo, P. Bugert, B. Wappenschmidt, et al. Association of NCOA3 Polymorphisms with Breast Cancer Risk Clin. Cancer Res., March 15, 2005; 11(6): 2169 - 2174. [Abstract] [Full Text] [PDF]

				A. Arimura, M. van Peer, A. J. Schroder, and P. B. Rothman The Transcriptional Co-activator p/CIP (NCoA-3) Is Up-regulated by STAT6 and Serves as a Positive Regulator of Transcriptional Activation by STAT6 J. Biol. Chem., July 23, 2004; 279(30): 31105 - 31112. [Abstract] [Full Text] [PDF]

				A. Goel and R. Janknecht Concerted Activation of ETS Protein ER81 by p160 Coactivators, the Acetyltransferase p300 and the Receptor Tyrosine Kinase HER2/Neu J. Biol. Chem., April 9, 2004; 279(15): 14909 - 14916. [Abstract] [Full Text] [PDF]

				C. M. Litterst, S. Kliem, D. Marilley, and E. Pfitzner NCoA-1/SRC-1 Is an Essential Coactivator of STAT5 That Binds to the FDL Motif in the {alpha}-Helical Region of the STAT5 Transactivation Domain J. Biol. Chem., November 14, 2003; 278(46): 45340 - 45351. [Abstract] [Full Text] [PDF]

				C. M. Litterst and E. Pfitzner Transcriptional Activation by STAT6 Requires the Direct Interaction with NCoA-1 J. Biol. Chem., November 30, 2001; 276(49): 45713 - 45721. [Abstract] [Full Text] [PDF]

				R. Reiter, A. Wellstein, and A. T. Riegel An Isoform of the Coactivator AIB1 That Increases Hormone and Growth Factor Sensitivity Is Overexpressed in Breast Cancer J. Biol. Chem., October 19, 2001; 276(43): 39736 - 39741. [Abstract] [Full Text] [PDF]

				A. Aranda and A. Pascual Nuclear Hormone Receptors and Gene Expression Physiol Rev, July 1, 2001; 81(3): 1269 - 1304. [Abstract] [Full Text] [PDF]

				S.-K. Lee, S.-Y. Jung, Y.-S. Kim, S.-Y. Na, Y. C. Lee, and J. W. Lee Two Distinct Nuclear Receptor-Interaction Domains and CREB-Binding Protein-Dependent Transactivation Function of Activating Signal Cointegrator-2 Mol. Endocrinol., February 1, 2001; 15(2): 241 - 254. [Abstract] [Full Text]

				S. M. Townson, T. Sullivan, Q. Zhang, G. M. Clark, C. K. Osborne, A. V. Lee, and S. Oesterreich HET/SAF-B Overexpression Causes Growth Arrest and Multinuclearity and Is Associated with Aneuploidy in Human Breast Cancer Clin. Cancer Res., September 1, 2000; 6(9): 3788 - 3796. [Abstract] [Full Text]

				S.-K. Lee, S.-Y. Na, S.-Y. Jung, J.-E. Choi, B. H. Jhun, J. Cheong, P. S. Meltzer, Y. C. Lee, and J. W. Lee Activating Protein-1, Nuclear Factor-{kappa}B, and Serum Response Factor as Novel Target Molecules of the Cancer-Amplified Transcription Coactivator ASC-2 Mol. Endocrinol., June 1, 2000; 14(6): 915 - 925. [Abstract] [Full Text]

				B. Belandia and M. G. Parker Functional Interaction between the p160 Coactivator Proteins and the Transcriptional Enhancer Factor Family of Transcription Factors J. Biol. Chem., September 29, 2000; 275(40): 30801 - 30805. [Abstract] [Full Text] [PDF]

				S.-K. Lee, B. Lee, and J. W. Lee Mutations in Retinoid X Receptor That Impair Heterodimerization with Specific Nuclear Hormone Receptor J. Biol. Chem., October 20, 2000; 275(43): 33522 - 33526. [Abstract] [Full Text] [PDF]

				H.-J. List, K. J. Lauritsen, R. Reiter, C. Powers, A. Wellstein, and A. T. Riegel Ribozyme Targeting Demonstrates That the Nuclear Receptor Coactivator AIB1 Is a Rate-limiting Factor for Estrogen-dependent Growth of Human MCF-7 Breast Cancer Cells J. Biol. Chem., June 22, 2001; 276(26): 23763 - 23768. [Abstract] [Full Text] [PDF]