help button home button Endocrine Society Molecular Endocrinology ENDO 08 Sessions Library
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Schaufele, F.
Right arrow Articles by McDonnell, D. P.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Schaufele, F.
Right arrow Articles by McDonnell, D. P.
Molecular Endocrinology 14 (12): 2024-2039
Copyright © 2000 by The Endocrine Society

Temporally Distinct and Ligand-Specific Recruitment of Nuclear Receptor-Interacting Peptides and Cofactors to Subnuclear Domains Containing the Estrogen Receptor

Fred Schaufele, Ching-yi Chang, Weiqun Liu, John D. Baxter1, Steven K. Nordeen, Yihong Wan, Richard N. Day and Donald P. McDonnell

Metabolic Research Unit and Department of Medicine (F.S., W.L., J.D.B.) University of California San Francisco, California 94143
Department of Pharmacology and Cancer Biology (C.-y.C., D.P.M.) Duke University Medical Center Durham, North Carolina 27710
Department of Pathology and Program in Molecular Biology (S.K.N., Y.W.) University of Colorado Health Sciences Center Denver, Colorado 80262 Departments of Medicine and Cell Biology (R.N.D.) National Science Foundation Center for Biological Timing University of Virginia Health Sciences Center Charlottesville, Virginia, 22908

Ligand binding to estrogen receptor (ER) is presumed to regulate the type and timing of ER interactions with different cofactors. Using fluorescence microscopy in living cells, we characterized the recruitment of five different green fluorescent protein (GFP)-labeled ER-interacting peptides to the distinct subnuclear compartment occupied by blue fluorescent protein (BFP)-labeled ER{alpha}. Different ligands promoted the recruitment of different peptides. One peptide was recruited in response to estradiol (E2), tamoxifen, raloxifene, or ICI 182,780 incubation whereas other peptides were recruited specifically by E2 or tamoxifen. Peptides containing different sequences surrounding the ER-interacting motif LXXLL were recruited with different time courses after E2 addition. Complex temporal kinetics also were observed for recruitment of the full-length, ER cofactor glucocorticoid receptor-interacting protein 1 (GRIP1); rapid, E2-dependent recruitment of GRIP1 was blocked by mutation of the GRIP1 LXXLL motifs to LXXAA whereas slower E2 recruitment persisted for the GRIP1 LXXAA mutant. This suggested the presence of multiple, temporally distinct GRIP 1 recruitment mechanisms. E2 recruitment of GRIP1 and LXXLL peptides was blocked by coincubation with excess ICI 182,780. In contrast, preformed E2/ER/GRIP1 and E2/ER/LXXLL complexes were resistant to subsequent ICI 182,780 addition whereas ICI 182,780 dispersed preformed complexes containing the GRIP1 LXXAA mutant. This suggested that E2-induced LXXLL binding altered subsequent ligand/ER interactions. Thus, alternative, ligand-selective recruitment and dissociation mechanisms with distinct temporal sequences are available for ER{alpha} action in vivo.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
I. S. Fenne, T. Hoang, M. Hauglid, J. V. Sagen, E. A. Lien, and G. Mellgren
Recruitment of Coactivator Glucocorticoid Receptor Interacting Protein 1 to an Estrogen Receptor Transcription Complex Is Regulated by the 3',5'-Cyclic Adenosine 5'-Monophosphate-Dependent Protein Kinase
Endocrinology, September 1, 2008; 149(9): 4336 - 4345.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. Sanchez, K. Sauve, N. Picard, and A. Tremblay
The Hormonal Response of Estrogen Receptor beta Is Decreased by the Phosphatidylinositol 3-Kinase/Akt Pathway via a Phosphorylation-dependent Release of CREB-binding Protein
J. Biol. Chem., February 16, 2007; 282(7): 4830 - 4840.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
A. Padron, L. Li, E. M. Kofoed, and F. Schaufele
Ligand-Selective Interdomain Conformations of Estrogen Receptor-{alpha}
Mol. Endocrinol., January 1, 2007; 21(1): 49 - 61.
[Abstract] [Full Text] [PDF]

Home page
 EndocrinologyHome page
T. Tomaru, T. Satoh, S. Yoshino, T. Ishizuka, K. Hashimoto, T. Monden, M. Yamada, and M. Mori
Isolation and Characterization of a Transcriptional Cofactor and Its Novel Isoform that Bind the Deoxyribonucleic Acid-Binding Domain of Peroxisome Proliferator-Activated Receptor-{gamma}
Endocrinology, January 1, 2006; 147(1): 377 - 388.
[Abstract] [Full Text] [PDF]

Home page
 J Biomol ScreenHome page
C. J. Larson, D. L. Osburn, K. Schmitz, L. Giampa, S.-M. Mong, K. Marschke, H. M. Seidel, J. Rosen, and A. Negro-Vilar
Peptide Binding Identifies an ER{alpha} Conformation That Generates Selective Activity in Multiple In Vitro Assays
J Biomol Screen, September 1, 2005; 10(6): 590 - 598.
[Abstract] [PDF]

Home page
 EndocrinologyHome page
T. Takahashi, M. Ohmichi, J. Kawagoe, C. Ohshima, M. Doshida, T. Ohta, M. Saitoh, A. Mori-Abe, B. Du, H. Igarashi, et al.
Growth Factors Change Nuclear Distribution of Estrogen Receptor-{alpha} via Mitogen-Activated Protein Kinase or Phosphatidylinositol 3-Kinase Cascade in a Human Breast Cancer Cell Line
Endocrinology, September 1, 2005; 146(9): 4082 - 4089.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
R. N. Day and F. Schaufele
Imaging Molecular Interactions in Living Cells
Mol. Endocrinol., July 1, 2005; 19(7): 1675 - 1686.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
K. Kim, N. Thu, B. Saville, and S. Safe
Domains of Estrogen Receptor {alpha} (ER{alpha}) Required for ER{alpha}/Sp1-Mediated Activation of GC-Rich Promoters by Estrogens and Antiestrogens in Breast Cancer Cells
Mol. Endocrinol., May 1, 2003; 17(5): 804 - 817.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
Y. Bai and V. Giguere
Isoform-Selective Interactions between Estrogen Receptors and Steroid Receptor Coactivators Promoted by Estradiol and ErbB-2 Signaling in Living Cells
Mol. Endocrinol., April 1, 2003; 17(4): 589 - 599.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
J. F. Enwright III, M. A. Kawecki-Crook, T. C. Voss, F. Schaufele, and R. N. Day
A PIT-1 Homeodomain Mutant Blocks the Intranuclear Recruitment Of the CCAAT/Enhancer Binding Protein {alpha} Required for Prolactin Gene Transcription
Mol. Endocrinol., February 1, 2003; 17(2): 209 - 222.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
O. J. Rivera, C. S. Song, V. E. Centonze, J. D. Lechleiter, B. Chatterjee, and A. K. Roy
Role of the Promyelocytic Leukemia Body in the Dynamic Interaction between the Androgen Receptor and Steroid Receptor Coactivator-1 in Living Cells
Mol. Endocrinol., January 1, 2003; 17(1): 128 - 140.
[Abstract] [Full Text] [PDF]

Home page
 Genes Dev.Home page
J.-B. Lazaro, P. J. Bailey, and A. B. Lassar
Cyclin D-cdk4 activity modulates the subnuclear localization and interaction of MEF2 with SRC-family coactivators during skeletal muscle differentiation
Genes & Dev., July 15, 2002; 16(14): 1792 - 1805.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
M. M. Gonzalez and C. Carlberg
Cross-repression, a Functional Consequence of the Physical Interaction of Non-liganded Nuclear Receptors and POU Domain Transcription Factors
J. Biol. Chem., May 17, 2002; 277(21): 18501 - 18509.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
R. V. Weatherman, C.-Y. Chang, N. J. Clegg, D. C. Carroll, R. N. Day, J. D. Baxter, D. P. McDonnell, T. S. Scanlan, and F. Schaufele
Ligand-Selective Interactions of ER Detected in Living Cells by Fluorescence Resonance Energy Transfer
Mol. Endocrinol., March 1, 2002; 16(3): 487 - 496.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Tung, T. Shen, M. G. Abel, R. L. Powell, G. S. Takimoto, C. A. Sartorius, and K. B. Horwitz
Mapping the Unique Activation Function 3 in the Progesterone B-receptor Upstream Segment. TWO LXXLL MOTIFS AND A TRYPTOPHAN RESIDUE ARE REQUIRED FOR ACTIVITY
J. Biol. Chem., October 19, 2001; 276(43): 39843 - 39851.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Endocrinol.Home page
F. Schaufele, J. F. Enwright III, X. Wang, C. Teoh, R. Srihari, R. Erickson, O. A. MacDougald, and R. N. Day
CCAAT/Enhancer Binding Protein {alpha} Assembles Essential Cooperating Factors in Common Subnuclear Domains
Mol. Endocrinol., October 1, 2001; 15(10): 1665 - 1676.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
A. Warnmark, T. Almlof, J. Leers, J.-A. Gustafsson, and E. Treuter
Differential Recruitment of the Mammalian Mediator Subunit TRAP220 by Estrogen Receptors ERalpha and ERbeta
J. Biol. Chem., June 22, 2001; 276(26): 23397 - 23404.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2000 by The Endocrine Society