| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Division of Endocrinology (R.J.U., Y.B., S.G.) Department of
Internal Medicine University of Texas Medical Branch Galveston,
Texas 77555-1060
Department of Human Biological Chemistry and
Genetics (A.K., T.G.W.) University of Texas Medical Branch
Galveston, Texas 77555-0645
The porcine P-450 cholesterol side-chain cleavage enzyme gene (P450scc) contains a 30-bp region [insulin-like growth factor response element (IGFRE)] that mediates insulin-like growth factor I (IGF-I)-stimulated gene expression and binds Sp1. In this study, we showed that polypyrimidine tract-binding protein (PTB)-associated splicing factor (PSF), an RNA-binding component of spliceosomes, binds to the IGFRE. Southwestern analysis with an IGFRE oligonucleotide showed that a protein (from Sp1-immunodepleted HeLa extract) fractionated on SDS-PAGE at 100 kDa. Microsequence analysis of 100-kDa band HeLa proteins detected PSF. DNA affinity chromatography, using an IGFRE mutant oligonucleotide that does not bind Sp1, isolated a protein that immunoreacted with PSF antibody. Deoxyribonuclease I (DNase I) footprint analysis showed recombinant PSF binds 5' of the Sp1-binding GC box of the IGFRE, and mutant oligonucleotides further delineated this region to a palindrome, CTGAGTC. Functional analysis of these mutants by transfection experiments in a cell line overexpressing the IGF-I receptor (NWTb3) found that an inability to bind PSF significantly increased the IGFRE transcriptional activity, while retaining responsiveness to IGF-I. Moreover, transfection of expression vectors for Sp1 and PSF in porcine granulosa cells found that Sp1 expression stimulated IGFRE transcriptional activity while PSF inhibited activity even with coexpression of Sp1. In conclusion, we identified PSF as an independent, inhibitory regulator of the transcriptional activity of the porcine P450scc IGFRE.
This article has been cited by other articles:
 |
|
 |
|
  Y. Morozumi, Y. Takizawa, M. Takaku, and H. Kurumizaka Human PSF binds to RAD51 and modulates its homologous-pairing and strand-exchange activities Nucleic Acids Res., May 15, 2009; (2009) gkp298v1. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. Natesampillai, J. Kerkvliet, P. C. K. Leung, and J. D. Veldhuis Regulation of Kruppel-like factor 4, 9, and 13 genes and the steroidogenic genes LDLR, StAR, and CYP11A in ovarian granulosa cells Am J Physiol Endocrinol Metab, February 1, 2008; 294(2): E385 - E391. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Galietta, R. H. Gunby, S. Redaelli, P. Stano, C. Carniti, A. Bachi, P. W. Tucker, C. J. Tartari, C.-J. Huang, E. Colombo, et al. NPM/ALK binds and phosphorylates the RNA/DNA-binding protein PSF in anaplastic large-cell lymphoma Blood, October 1, 2007; 110(7): 2600 - 2609. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X. Dong, J. Sweet, J. R. G. Challis, T. Brown, and S. J. Lye Transcriptional Activity of Androgen Receptor Is Modulated by Two RNA Splicing Factors, PSF and p54nrb Mol. Cell. Biol., July 1, 2007; 27(13): 4863 - 4875. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Deisseroth Normal and pathological functions of mammalian retroelements PNAS, August 30, 2005; 102(35): 12292 - 12293. [Full Text] [PDF]
|
|
|
|
|
|
X. Song, Y. Sun, and A. Garen From The Cover: Roles of PSF protein and VL30 RNA in reversible gene regulation PNAS, August 23, 2005; 102(34): 12189 - 12193. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
E. Rosonina, J. Y. Y. Ip, J. A. Calarco, M. A. Bakowski, A. Emili, S. McCracken, P. Tucker, C. J. Ingles, and B. J. Blencowe Role for PSF in Mediating Transcriptional Activator-Dependent Stimulation of Pre-mRNA Processing In Vivo Mol. Cell. Biol., August 1, 2005; 25(15): 6734 - 6746. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 R. Myojin, S. Kuwahara, T. Yasaki, T. Matsunaga, T. Sakurai, M. Kimura, S. Uesugi, and Y. Kurihara Expression and Functional Significance of Mouse Paraspeckle Protein 1 on Spermatogenesis Biol Reprod, September 1, 2004; 71(3): 926 - 932. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Urban, Y. H. Bodenburg, J. Jiang, L. Denner, and J. Chedrese Protein kinase C{iota} enhances the transcriptional activity of the porcine P-450 side-chain cleavage insulin-like response element Am J Physiol Endocrinol Metab, June 1, 2004; 286(6): E975 - E979. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 F. Hammer, N. A. Compagnone, J.-L. Vigne, S. R. Bair, and S. H. Mellon Transcriptional Regulation of P450scc Gene Expression in the Embryonic Rodent Nervous System Endocrinology, February 1, 2004; 145(2): 901 - 912. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Song, A. Sui, and A. Garen Binding of mouse VL30 retrotransposon RNA to PSF protein induces genes repressed by PSF: Effects on steroidogenesis and oncogenesis PNAS, January 13, 2004; 101(2): 621 - 626. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Urban and Y. Bodenburg PTB-associated splicing factor regulates growth factor-stimulated gene expression in mammalian cells Am J Physiol Endocrinol Metab, October 1, 2002; 283(4): E794 - E798. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. J. Urban, Y. H. Bodenburg, and T. G. Wood NH2 terminus of PTB-associated splicing factor binds to the porcine P450scc IGF-I response element Am J Physiol Endocrinol Metab, September 1, 2002; 283(3): E423 - E427. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Sewer and M. R. Waterman Adrenocorticotropin/Cyclic Adenosine 3',5'-Monophosphate-Mediated Transcription of the Human CYP17 Gene in the Adrenal Cortex Is Dependent on Phosphatase Activity Endocrinology, May 1, 2002; 143(5): 1769 - 1777. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. B. Sewer, V. Q. Nguyen, C.-J. Huang, P. W. Tucker, N. Kagawa, and M. R. Waterman Transcriptional Activation of Human CYP17 in H295R Adrenocortical Cells Depends on Complex Formation among p54nrb/NonO, Protein-Associated Splicing Factor, and SF-1, a Complex That Also Participates in Repression of Transcription Endocrinology, April 1, 2002; 143(4): 1280 - 1290. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-z. Lin, H. Takemori, Y. Katoh, J. Doi, N. Horike, A. Makino, Y. Nonaka, and M. Okamoto Salt-Inducible Kinase Is Involved in the ACTH/cAMP-Dependent Protein Kinase Signaling in Y1 Mouse Adrenocortical Tumor Cells Mol. Endocrinol., August 1, 2001; 15(8): 1264 - 1276. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 Y. Shav-Tal, M. Cohen, S. Lapter, B. Dye, J. G. Patton, J. Vandekerckhove, and D. Zipori Nuclear Relocalization of the Pre-mRNA Splicing Factor PSF during Apoptosis Involves Hyperphosphorylation, Masking of Antigenic Epitopes, and Changes in Protein Interactions Mol. Biol. Cell, August 1, 2001; 12(8): 2328 - 2340. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
M. Mathur, P. W. Tucker, and H. H. Samuels PSF Is a Novel Corepressor That Mediates Its Effect through Sin3A and the DNA Binding Domain of Nuclear Hormone Receptors Mol. Cell. Biol., April 1, 2001; 21(7): 2298 - 2311. [Abstract] [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
