This Article Full Text Full Text (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Lee, S.-K. Articles by Lee, J. W. Search for Related Content PubMed PubMed Citation Articles by Lee, S.-K. Articles by Lee, J. W.

Activating Protein-1, Nuclear Factor-B, and Serum Response Factor as Novel Target Molecules of the Cancer-Amplified Transcription Coactivator ASC-2

Center for Ligand and Transcription (S.-K.L., S.-Y.N., S.-Y.J., J.H.C., Y.C.L., J.W.L.) Department of Biology (S.-K.L., S.-Y.N.) and Hormone Research Center (J.H.C., Y.C.L., J.W.L.) Chonnam National University Kwangju 500–757, Korea College of Pharmacy (J.-E.C., B.H.J.) Pusan National University Pusan 609–735, Korea Cancer Genetics Branch (P.S.M.) National Human Genome Research Institute National Institutes of Health Bethesda, Maryland 20892-4470

ASC-2 was recently discovered as a cancer-amplified transcription coactivator molecule of nuclear receptors, which interacts with multifunctional transcription integrators steroid receptor coactivator-1 (SRC-1) and CREB-binding protein (CBP)/p300. Herein, we report the identification of three mitogenic transcription factors as novel target molecules of ASC-2. First, the C-terminal transactivation domain of serum response factor (SRF) was identified among a series of ASC-2-interacting proteins from the yeast two-hybrid screening. Second, ASC-2 specifically interacted with the activating protein-1 (AP-1) components c-Jun and c-Fos as well as the nuclear factor-B (NFB) components p50 and p65, as demonstrated by the glutathione S-transferase pull-down assays as well as the yeast two-hybrid tests. In cotransfection of mammalian cells, ASC-2 potentiated transactivations by SRF, AP-1, and NFB in a dose-dependent manner, either alone or in conjunction with SRC-1 and p300. In addition, ASC-2 efficiently relieved the previously described transrepression between nuclear receptors and either AP-1 or NFB. Overall, these results suggest that the nuclear receptor coactivator ASC-2 also mediates transactivations by SRF, AP-1, and NFB, which may contribute to the putative, ASC-2-mediated tumorigenesis.

This article has been cited by other articles:

				M. A. Mahajan, A. Murray, D. Levy, and H. H. Samuels Nuclear Receptor Coregulator (NRC): Mapping of the Dimerization Domain, Activation of p53 and STAT-2, and Identification of the Activation Domain AD2 Necessary for Nuclear Receptor Signaling Mol. Endocrinol., August 1, 2007; 21(8): 1822 - 1834. [Abstract] [Full Text] [PDF]

				B.-G. Ju, V. V. Lunyak, V. Perissi, I. Garcia-Bassets, D. W. Rose, C. K. Glass, and M. G. Rosenfeld A topoisomerase IIbeta-mediated dsDNA break required for regulated transcription. Science, June 23, 2006; 312(5781): 1798 - 1802. [Abstract] [Full Text] [PDF]

				A. Murakami, T. Shigemori, and H. Ohigashi Zingiberaceous and Citrus Constituents, 1'-Acetoxychavicol Acetate, Zerumbone, Auraptene, and Nobiletin, Suppress Lipopolysaccharide-Induced Cyclooxygenase-2 Expression in RAW264.7 Murine Macrophages through Different Modes of Action J. Nutr., December 1, 2005; 135(12): 2987S - 2992S. [Abstract] [Full Text] [PDF]

				E. Choi, S. Lee, S.-Y. Yeom, G. H. Kim, J. W. Lee, and S.-W. Kim Characterization of Activating Signal Cointegrator-2 as a Novel Transcriptional Coactivator of the Xenobiotic Nuclear Receptor Constitutive Androstane Receptor Mol. Endocrinol., July 1, 2005; 19(7): 1711 - 1719. [Abstract] [Full Text] [PDF]

				M. A. Mahajan and H. H. Samuels Nuclear Hormone Receptor Coregulator: Role in Hormone Action, Metabolism, Growth, and Development Endocr. Rev., June 1, 2005; 26(4): 583 - 597. [Abstract] [Full Text] [PDF]

				I. Quelo, C. Gauthier, G. E. Hannigan, S. Dedhar, and R. St-Arnaud Integrin-linked Kinase Regulates the Nuclear Entry of the c-Jun Coactivator {alpha}-NAC and Its Coactivation Potency J. Biol. Chem., October 15, 2004; 279(42): 43893 - 43899. [Abstract] [Full Text] [PDF]

				H. Zhang, S.-Q. Kuang, L. Liao, S. Zhou, and J. Xu Haploid Inactivation of the Amplified-in-Breast Cancer 3 Coactivator Reduces the Inhibitory Effect of Peroxisome Proliferator-Activated Receptor {gamma} and Retinoid X Receptor on Cell Proliferation and Accelerates Polyoma Middle-T Antigen-Induced Mammary Tumorigenesis in Mice Cancer Res., October 1, 2004; 64(19): 7169 - 7177. [Abstract] [Full Text] [PDF]

				N. Yin, D. Wang, H. Zhang, X. Yi, X. Sun, B. Shi, H. Wu, G. Wu, X. Wang, and Y. Shang Molecular Mechanisms Involved in the Growth Stimulation of Breast Cancer Cells by Leptin Cancer Res., August 15, 2004; 64(16): 5870 - 5875. [Abstract] [Full Text] [PDF]

				S. Hong, H. M. Choi, M. J. Park, Y. H. Kim, Y. H. Choi, H. H. Kim, Y. H. Choi, and J. Cheong Activation and Interaction of ATF2 with the Coactivator ASC-2 Are Responsive for Granulocytic Differentiation by Retinoic Acid J. Biol. Chem., April 23, 2004; 279(17): 16996 - 17003. [Abstract] [Full Text] [PDF]

				M. Oukka, M. N. Wein, and L. H. Glimcher Schnurri-3 (KRC) Interacts with c-Jun to Regulate the IL-2 Gene in T Cells J. Exp. Med., January 5, 2004; 199(1): 15 - 24. [Abstract] [Full Text] [PDF]

				C. M. Litterst, S. Kliem, D. Marilley, and E. Pfitzner NCoA-1/SRC-1 Is an Essential Coactivator of STAT5 That Binds to the FDL Motif in the {alpha}-Helical Region of the STAT5 Transactivation Domain J. Biol. Chem., November 14, 2003; 278(46): 45340 - 45351. [Abstract] [Full Text] [PDF]

				S.-W. Kim, K. Park, E. Kwak, E. Choi, S. Lee, J. Ham, H. Kang, J. M. Kim, S. Y. Hwang, Y.-Y. Kong, et al. Activating Signal Cointegrator 2 Required for Liver Lipid Metabolism Mediated by Liver X Receptors in Mice Mol. Cell. Biol., May 15, 2003; 23(10): 3583 - 3592. [Abstract] [Full Text] [PDF]

				H. Zhang, L. Liao, S.-Q. Kuang, and J. Xu Spatial Distribution of the Messenger Ribonucleic Acid and Protein of the Nuclear Receptor Coactivator, Amplified in Breast Cancer-3, in Mice Endocrinology, April 1, 2003; 144(4): 1435 - 1443. [Abstract] [Full Text] [PDF]

				S. V. Komarova, M. F. Pilkington, A. F. Weidema, S. J. Dixon, and S. M. Sims RANK Ligand-induced Elevation of Cytosolic Ca2+ Accelerates Nuclear Translocation of Nuclear Factor kappa B in Osteoclasts J. Biol. Chem., February 28, 2003; 278(10): 8286 - 8293. [Abstract] [Full Text] [PDF]

				Y.-H. Goo, Y. C. Sohn, D.-H. Kim, S.-W. Kim, M.-J. Kang, D.-J. Jung, E. Kwak, N. A. Barlev, S. L. Berger, V. T. Chow, et al. Activating Signal Cointegrator 2 Belongs to a Novel Steady-State Complex That Contains a Subset of Trithorax Group Proteins Mol. Cell. Biol., January 1, 2003; 23(1): 140 - 149. [Abstract] [Full Text]

				S.-Q. Kuang, L. Liao, H. Zhang, F. A. Pereira, Y. Yuan, F. J. DeMayo, L. Ko, and J. Xu Deletion of the Cancer-amplified Coactivator AIB3 Results in Defective Placentation and Embryonic Lethality J. Biol. Chem., November 15, 2002; 277(47): 45356 - 45360. [Abstract] [Full Text] [PDF]

				D.-J. Jung, H.-S. Sung, Y.-W. Goo, H. M. Lee, O. K. Park, S.-Y. Jung, J. Lim, H.-J. Kim, S.-K. Lee, T. S. Kim, et al. Novel Transcription Coactivator Complex Containing Activating Signal Cointegrator 1 Mol. Cell. Biol., July 15, 2002; 22(14): 5203 - 5211. [Abstract] [Full Text] [PDF]

				D. A. Tice, I. Soloviev, and P. Polakis Activation of the Wnt Pathway Interferes with Serum Response Element-driven Transcription of Immediate Early Genes J. Biol. Chem., February 15, 2002; 277(8): 6118 - 6123. [Abstract] [Full Text] [PDF]

				Y. C. Sohn, E. Kwak, Y. Na, J. W. Lee, and S.-K. Lee Silencing Mediator of Retinoid and Thyroid Hormone Receptors and Activating Signal Cointegrator-2 as Transcriptional Coregulators of the Orphan Nuclear Receptor Nur77 J. Biol. Chem., November 16, 2001; 276(47): 43734 - 43739. [Abstract] [Full Text] [PDF]

				S.-K. Lee, S.-Y. Jung, Y.-S. Kim, S.-Y. Na, Y. C. Lee, and J. W. Lee Two Distinct Nuclear Receptor-Interaction Domains and CREB-Binding Protein-Dependent Transactivation Function of Activating Signal Cointegrator-2 Mol. Endocrinol., February 1, 2001; 15(2): 241 - 254. [Abstract] [Full Text]

				L. J. Nissen, J.-C. Gelly, and R. A. Hipskind Induction-independent Recruitment of CREB-binding Protein to the c-fos Serum Response Element through Interactions between the Bromodomain and Elk-1 J. Biol. Chem., February 9, 2001; 276(7): 5213 - 5221. [Abstract] [Full Text] [PDF]

				M. K. Jang, Y. H. Goo, Y. C. Sohn, Y. S. Kim, S.-K. Lee, H. Kang, J. Cheong, and J. W. Lee Ca2+/Calmodulin-dependent Protein Kinase IV Stimulates Nuclear Factor-kappa B Transactivation via Phosphorylation of the p65 Subunit J. Biol. Chem., June 1, 2001; 276(23): 20005 - 20010. [Abstract] [Full Text] [PDF]

				N. V. Kumar and L. R. Bernstein Ten ERK-related Proteins in Three Distinct Classes Associate with AP-1 Proteins and/or AP-1 DNA J. Biol. Chem., August 17, 2001; 276(34): 32362 - 32372. [Abstract] [Full Text] [PDF]

				D.-J. Jung, S.-Y. Na, D. S. Na, and J. W. Lee Molecular Cloning and Characterization of CAPER, a Novel Coactivator of Activating Protein-1 and Estrogen Receptors J. Biol. Chem., January 4, 2002; 277(2): 1229 - 1234. [Abstract] [Full Text] [PDF]