help button home button Endocrine Society Molecular Endocrinology ENDO 08 Sessions Library
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
This Article
Right arrow Full Text
Right arrow Full Text (PDF)
Right arrow Purchase Article
Right arrow View Shopping Cart
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Hovey, R. C.
Right arrow Articles by Vonderhaar, B. K.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Hovey, R. C.
Right arrow Articles by Vonderhaar, B. K.
Molecular Endocrinology 15 (5): 819-831
Copyright © 2001 by The Endocrine Society

Transcriptional Regulation of Vascular Endothelial Growth Factor Expression in Epithelial and Stromal Cells during Mouse Mammary Gland Development

Russell C. Hovey1, Anita S. Goldhar1, Judit Baffi and Barbara K. Vonderhaar

Laboratory of Tumor Immunology and Biology (R.C.H., A.S.G., B.K.V.) National Cancer Institute and Laboratory of Immunology (J.B.) National Eye Institute National Institutes of Health Bethesda, Maryland 20892

Accompanying changes in the development and function of the mammary gland is the establishment of a vascular network of critical importance for lactogenesis and tumorigenesis. A potent angiogenic and permeability factor that regulates vascular development in association with epithelial-stromal interactions is vascular endothelial growth factor (VEGF). Analysis of VEGF transcription by RT-PCR revealed mRNA for all three VEGF isoforms (VEGF120, 164, 188) within the mammary gland of nulliparous females. During pregnancy the level of VEGF188 declined and became undetectable during lactation in association with the increased abundance of VEGF120 and VEGF164 mRNAs. All three isoforms were expressed at consistent levels within the cleared mammary fat pad throughout development. Furthermore, the presence of VEGF188 mRNA in omental adipose tissue at various stages established that VEGF188 is expressed specifically in adipose tissue within the mammary gland. Using 3T3-L1 preadipocytes it was demonstrated that VEGF188 mRNA transcription occurs as a late event during lipogenesis distinct from earlier induction of VEGF120 and VEGF164 mRNA during differentiation. In contrast, HC11 mammary epithelial cells only expressed mRNA for VEGF120 and VEGF164. Localization of VEGF mRNA and protein revealed that VEGF is expressed in stromal cells of the mammary gland in nulliparous females and then undergoes a transition to epithelial expression during lactation. By contrast, mRNA for the VEGF receptors, Flk-1 and Flt-1, localized to stromal cells within the mammary fat pad during virgin and gestational development and was expressed in the interstitial tissue basal to epithelial cells during lactation. Taken together, these results support the conclusion that VEGF is differentially transcribed by specific cell types within the mammary gland, and that under hormonal regulation it functions in an autocrine/paracrine manner.




This article has been cited by other articles:


Home page
 FASEB J.Home page
H. Rossiter, C. Barresi, M. Ghannadan, F. Gruber, M. Mildner, D. Fodinger, and E. Tschachler
Inactivation of VEGF in mammary gland epithelium severely compromises mammary gland development and function
FASEB J, December 1, 2007; 21(14): 3994 - 4004.
[Abstract] [Full Text] [PDF]

Home page
 J DAIRY SCIHome page
B. E. Dallard, V. Ruffino, S. Heffel, and L. F. Calvinho
Effect of a Biological Response Modifier on Expression of Growth Factors and Cellular Proliferation at Drying Off
J Dairy Sci, May 1, 2007; 90(5): 2229 - 2240.
[Abstract] [Full Text] [PDF]

Home page
 Endocr Relat CancerHome page
E. Ueda, U. Ozerdem, Y.-H. Chen, M. Yao, K. T. Huang, H. Sun, M. Martins-Green, P. Bartolini, and A. M Walker
A molecular mimic demonstrates that phosphorylated human prolactin is a potent anti-angiogenic hormone.
Endocr. Relat. Cancer, March 1, 2006; 13(1): 95 - 111.
[Abstract] [Full Text] [PDF]

Home page
 J. Lipid Res.Home page
S. Chandrasekharan, N. A. Foley, L. Jania, P. Clark, L. P. Audoly, and B. H. Koller
Coupling of COX-1 to mPGES1 for prostaglandin E2 biosynthesis in the murine mammary gland
J. Lipid Res., December 1, 2005; 46(12): 2636 - 2648.
[Abstract] [Full Text] [PDF]

Home page
 Biol. Reprod.Home page
M. Tesone, R. L. Stouffer, S. M. Borman, J. D. Hennebold, and T. A. Molskness
Vascular Endothelial Growth Factor (VEGF) Production by the Monkey Corpus Luteum During the Menstrual Cycle: Isoform-Selective Messenger RNA Expression In Vivo and Hypoxia-Regulated Protein Secretion In Vitro
Biol Reprod, November 1, 2005; 73(5): 927 - 934.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
K. Nakagawa, Y. Sasaki, S. Kato, N. Kubodera, and T. Okano
22-Oxa-1{alpha},25-dihydroxyvitamin D3 inhibits metastasis and angiogenesis in lung cancer
Carcinogenesis, June 1, 2005; 26(6): 1044 - 1054.
[Abstract] [Full Text] [PDF]

Home page
 CarcinogenesisHome page
K. Nakagawa, A. Kawaura, S. Kato, E. Takeda, and T. Okano
1{alpha},25-Dihydroxyvitamin D3 is a preventive factor in the metastasis of lung cancer
Carcinogenesis, February 1, 2005; 26(2): 429 - 440.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
J. R. Beckley, B. U. Pauli, and R. C. Elble
Re-expression of Detachment-inducible Chloride Channel mCLCA5 Suppresses Growth of Metastatic Breast Cancer Cells
J. Biol. Chem., October 1, 2004; 279(40): 41634 - 41641.
[Abstract] [Full Text] [PDF]

Home page
 J. Biol. Chem.Home page
L. Wang, J. H. Kwak, S. I. Kim, Y. He, and M. E. Choi
Transforming Growth Factor-{beta}1 Stimulates Vascular Endothelial Growth Factor 164 via Mitogen-activated Protein Kinase Kinase 3-p38{alpha} and p38{delta} Mitogen-activated Protein Kinase-dependent Pathway in Murine Mesangial Cells
J. Biol. Chem., August 6, 2004; 279(32): 33213 - 33219.
[Abstract] [Full Text] [PDF]

Home page
 Proc. Natl. Acad. Sci. USAHome page
S.-H. Chang, C. H. Liu, R. Conway, D. K. Han, K. Nithipatikom, O. C. Trifan, T. F. Lane, and T. Hla
From the Cover: Role of prostaglandin E2-dependent angiogenic switch in cyclooxygenase 2-induced breast cancer progression
PNAS, January 13, 2004; 101(2): 591 - 596.
[Abstract] [Full Text] [PDF]

Home page
 J. Dent. Res.Home page
P.D.S. Telles, C.T. Hanks, M.A.A.M. Machado, and J.E. Nor
Lipoteichoic Acid Up-regulates VEGF Expression in Macrophages and Pulp Cells
J. Dent. Res., June 1, 2003; 82(6): 466 - 470.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Physiol. Endocrinol. Metab.Home page
C. Mallet, O. Feraud, G. Ouengue-Mbele, I. Gaillard, N. Sappay, D. Vittet, and I. Vilgrain
Differential expression of VEGF receptors in adrenal atrophy induced by dexamethasone: a protective role of ACTH
Am J Physiol Endocrinol Metab, January 1, 2003; 284(1): E156 - E167.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
P. A. Masso-Welch, D. Zangani, C. Ip, M. M. Vaughan, S. Shoemaker, R. A. Ramirez, and M. M. Ip
Inhibition of Angiogenesis by the Cancer Chemopreventive Agent Conjugated Linoleic Acid
Cancer Res., August 1, 2002; 62(15): 4383 - 4389.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 2001 by The Endocrine Society