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Maturity-Onset Diabetes of the Young Type 1 (MODY1)-Associated Mutations R154X and E276Q in Hepatocyte Nuclear Factor 4 (HNF4) Gene Impair Recruitment of p300, a Key Transcriptional Coactivator

Unité 459 INSERM Laboratoire de Biologie Cellulaire Université H. Warembourg Lille, France F 59045

Hepatocyte nuclear factor 4 (HNF4) is a nuclear receptor involved in glucose homeostasis and is required for normal ß-cell function. Mutations in the HNF4 gene are associated with maturity-onset diabetes of the young type 1. E276Q and R154X mutations were previously shown to impair intrinsic transcriptional activity (without exogenously supplied coactivators) of HNF4. Given that transcriptional partners of HNF4 modulate its intrinsic transcriptional activity and play crucial roles in HNF4 function, we investigated the effects of these mutations on potentiation of HNF4 activity by p300, a key coactivator for HNF4. We show here that loss of HNF4 function by both mutations is increased through impaired physical interaction and functional cooperation between HNF4 and p300. Impairment of p300-mediated potentiation of HNF4 transcriptional activity is of particular importance for the E276Q mutant since its intrinsic transcriptional activity is moderately affected. Together with previous results obtained with chicken ovalbumin upstream promoter-transcription factor II, our results highlight that impairment of recruitment of transcriptional partners represents an important mechanism leading to abnormal HNF4 function resulting from the MODY1 E276Q mutation. The impaired potentiations of HNF4 activity were observed on the promoter of HNF1, a transcription factor involved in a transcriptional network and required for ß-cell function. Given its involvement in a regulatory signaling cascade, loss of HNF4 function may cause reduced ß-cell function secondary to defective HNF1 expression. Our results also shed light on a better structure-function relationship of HNF4 and on p300 sequences involved in the interaction with HNF4.

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