This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Murphy, B. D. Articles by Sassone-Corsi, P. Search for Related Content PubMed PubMed Citation Articles by Murphy, B. D. Articles by Sassone-Corsi, P.

Heat Shock Interferes with Steroidogenesis by Reducing Transcription of the Steroidogenic Acute Regulatory Protein Gene

Institut de Génétique et Biologie Moléculaire et Cellulaire (B.D.M., E.L., P.S.-C.), Centre Nationale de la Recherche Scientifique-INSERM-ULP, Illkirch C.U. de Strasbourg, France 67404; Department of Cell Biology and Biochemistry (Z.L., J.S., L.P.W., D.M.S.), Texas Tech University Health Sciences Centre, Lubbock, Texas 79430; and Centre de recherche en reproduction animale (B.D.M.), Université de Montréal, St-Hyacinthe, Québec, Canada J2S 7C6

Address all correspondence and request for reprints to: Dr. Bruce D. Murphy,Centre de Recherche en Reproduction Animale, Universite de Montreal, 3200 Rue Sicotte, Quebec PQ Canada J2S 7C6. E-mail: murphyb{at}medvet.umontreal.ca

A key regulatory point in fine tuning of steroidogenesis is the synthesis of steroidogenic acute regulatory protein, which transfers cholesterol into mitochondria. Heat shock and toxic insults reduce steroidogenic acute regulatory protein, severely compromising steroid synthesis. As the molecular mechanisms for this reduction remain elusive, we tested the hypothesis that heat shock directly interferes with transcription of the steroidogenic acute regulatory protein gene. We show that, in mouse MA-10 Leydig tumor cells, heat shock caused drastic declines in (Bu)₂cAMP-induced progesterone accumulation and steroidogenic acute regulatory protein transcript abundance. A proximal steroidogenic acute regulatory protein promoter fragment (-85 to +39) is sufficient to direct both cAMP inducibility and heat shock inhibition. Nuclear extracts from MA-10 cells displayed binding to this proximal promoter fragment as a low mobility complex in gel shift experiments. This complex disappeared in nuclear extracts taken at 5 and 10 min after initiation of heat shock and reappeared in extracts taken at 2 and 8 h. Similar low- mobility complexes formed on oligonucleotides representing the overlapping subfragments of the minimal steroidogenic acute regulatory protein promoter fragment sensitive to the heat shock effect. Extracts from heat-shocked MA-10 cells displayed reduced complex formation to each of the subfragments. We conclude that heat shock reduces progesterone synthesis, steroidogenic acute regulatory protein mRNA abundance, and steroidogenic acute regulatory protein promoter activity and disrupts binding of nuclear proteins to the proximal region of the steroidogenic acute regulatory protein promoter. Together these observations provide strong evidence for a mechanism of transcriptional inhibition in the down-regulation of steroidogenic acute regulatory protein expression by heat shock.

This article has been cited by other articles:

				H. Taira and M. M. Beck Activity of Three-{beta}-Hydroxysteroid Dehydrogenase in Granulosa Cells Treated in Vitro with Luteinizing Hormone, Follicle-Stimulating Hormone, Prolactin, or a Combination. Poult. Sci., October 1, 2006; 85(10): 1769 - 1774. [Abstract] [Full Text] [PDF]

				N. Y. Gevry, E. Lalli, P. Sassone-Corsi, and B. D. Murphy Regulation of Niemann-Pick C1 Gene Expression by the 3'5'-Cyclic Adenosine Monophosphate Pathway in Steroidogenic Cells Mol. Endocrinol., April 1, 2003; 17(4): 704 - 715. [Abstract] [Full Text] [PDF]

				Y.-H. Lue, B. L. Lasley, L. S. Laughlin, R. S. Swerdloff, A. P. S. Hikim, A. Leung, J. W. Overstreet, and C. Wang Mild Testicular Hyperthermia Induces Profound Transitional Spermatogenic Suppression Through Increased Germ Cell Apoptosis in Adult Cynomolgus Monkeys (Macaca fascicularis)( J Androl, November 1, 2002; 23(6): 799 - 805. [Abstract] [Full Text] [PDF]