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The Department of Medicine, Banting and Best Diabetes Centre, Toronto General Hospital, University of Toronto, Toronto, Ontario, Canada M5G 2C4
Address all correspondence and requests for reprints to: Dr. Daniel J. Drucker, Banting and Best Diabetes Centre, Toronto General Hospital, 200 Elizabeth Street, Toronto, Ontario, Canada M5G 2C4. E-mail: d.drucker{at}utoronto.ca.
Tissue-specific proglucagon gene transcription is achieved through combinations of transcription factors expressed in pancreatic A cells and enteroendocrine L cells of the small and large intestine. Cell transfection and electrophoretic mobility shift assay experiments previously identified Pax-2 as a regulator of islet proglucagon gene expression. We examined whether Pax-2 regulates gut proglucagon gene expression using enteroendocrine cell lines and Pax21NEU mutant mice. Immunoreactive Pax-2 was detected in STC-1 enteroendocrine cells, and Pax-2 activated proglucagon promoter activity in transfected baby hamster kidney and GLUTag cells. Pax-2 antisera diminished the formation of a Pax-2-G3 complex in electrophoretic mobility shift assay studies using nuclear extracts from islet and enteroendocrine cell lines. Surprisingly, Pax-2 mRNA transcripts were not detected by RT-PCR in RNA isolated from adult rat pancreas, rat islets, embryonic d 19 or adult murine pancreas and gastrointestinal tract. Furthermore, embryonic d 19 or neonatal d 1 Pax21NEU mice exhibited normal islet A cells and gut endocrine L cells, and no decrement in pancreatic or intestinal glucagon gene expression. These findings demonstrate that Pax-2 is not essential for the developmental formation of islet A or gut L cells and does not play a role in the physiological control of proglucagon gene expression in vivo.
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 G. Flock, X. Cao, and D. J. Drucker Pdx-1 Is Not Sufficient for Repression of Proglucagon Gene Transcription in Islet or Enteroendocrine Cells Endocrinology, January 1, 2005; 146(1): 441 - 449. [Abstract] [Full Text] [PDF]
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X. Cao, G. Flock, C. Choi, D. M. Irwin, and D. J. Drucker Aberrant Regulation of Human Intestinal Proglucagon Gene Expression in the NCI-H716 Cell Line Endocrinology, May 1, 2003; 144(5): 2025 - 2033. [Abstract] [Full Text] [PDF]
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