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Coupling of GnRH Concentration and the GnRH Receptor-Activated Gene Program

Department of Neurology (T.Y., E.W., B.J.E., R.L.P., S.C.S.) Fishberg Research Center for Neurobiology (S.C.S.), Graduate School of Biological Sciences (F.R.) and Department of Pharmacology (S.C.S.), Mount Sinai School of Medicine, New York, New York 10029

Address all correspondence and requests for reprints to: Stuart C. Sealfon, M.D., Neurology Box 1137, Mount Sinai School of Medicine, New York, New York 10029. E-mail: Stuart.Sealfon{at}mssm.edu.

The initial waves of gene induction caused by GnRH in the LßT2 gonadotrope cell line have recently been identified using microarrays. We now investigate the relationship of the concentration of GnRH to the level of biosynthesis induced. Using an optimized custom cDNA microarray, we show that a large number of genes are induced in a concentration-dependent fashion. Detailed time course studies of the induction of six induced transcripts using quantitative real-time PCR suggest that the amplitude, but not the temporal pattern, depends on the concentration of GnRH. The early genes appear to show a delay in gene induction, followed by a linear phase of increase. The relationship of rate of synthesis and GnRH concentration was studied by mathematical modeling of the induction of two genes, gly96 and tis11. In both cases, only the rates of increase, but not the lag times, are influenced by the concentration of GnRH exposure. Western blot analyses for c-Jun and Egr1 show that the levels of nuclear protein for these transcription factors also depend on the concentration of GnRH. These studies indicate that, despite the complex signaling network connecting the receptor to the activated genes, the biosynthetic rate of RNA polymerase at induced genes is correlated with the concentration of GnRH at the GnRH receptor.

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