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Thyroid Hormone Receptor-ß Mutations Conferring Hormone Resistance and Reduced Corepressor Release Exhibit Decreased Stability in the N-Terminal Ligand-Binding Domain

Graduate Group in Biophysics (B.R.H.), Department of Physiology (M.D., H.A.I.), Metabolic Research Unit, Department of Medicine (B.L.W., S.T.C.-L., H.N., J.D.B.), and Department of Biochemistry and Biophysics (R.J.F.), University of California, San Francisco, California 94143-0448

Address all correspondence and requests for reprints to:Robert Fletterick, Department of Biochemistry and Biophysics, University of California, 513 Parnassus Avenue, Room S-1071, San Francisco, California 94143-0448. E-mail: flett{at}msg.ucsf.edu.

Resistance to thyroid hormone (RTH) syndrome is associated with mutations in the human thyroid hormone receptor-ß (hTRß), many of which show marked reduction in hormone binding. Here, we investigated the structural consequences of two RTH mutants (A234T and R243Q), residing in the flexible N-terminal portion of the ligand binding domain (LBD), which exhibit modestly reduced hormone binding with impaired release of corepressor. X-ray crystallography analyses revealed that these two RTH mutants modulate the position of this flexible region by either altering the movement of helix 1 (A234T) or disrupting a salt bridge (R243Q). The subsequent increased flexibility and mobility in regions after the two sites of mutation coincided with a disorganized LBD. Consistent with this finding, the ability of these mutant N-terminal regions (234–260) to recruit the remaining LBD was decreased in a ligand-dependent helix assembly assay. Collectively, these data suggest that structural information imparted by the flexible segment in the N-terminal LBD is critical for overall stability of the LBD. Thus, these structural analyses provide mechanistic insight into the etiology of RTH disease in human TRß mutants that exhibit hormone binding with decreased ligand-dependent corepressor release.

NURSA Molecule Pages Link:

Nuclear Receptors:   TRβ

Ligands:   Thyroid hormone

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