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Molecular Endocrinology, doi:10.1210/me.2002-0435
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Molecular Endocrinology 17 (6): 1117-1130
Copyright © 2003 by The Endocrine Society

Phosphodiesterase Regulation Is Critical for the Differentiation and Pattern of Gene Expression in Granulosa Cells of the Ovarian Follicle

Jy-Young Park1, Francois Richard1, Sang-Young Chun, Jeong-Hoh Park, Evelyn Law, Kathleen Horner, S-L Catherine Jin and Marco Conti

Division of Reproductive Biology (J.-Y.P., F.R., E.L., K.H., S.-L.C.J., M.C.), Department of Obstetrics and Gynecology, Stanford University, Stanford, California 94305; and Hormone Research Center (S.-Y.C., J.-H.P.), Chonnam National University, Kwangju 500-757, Republic of Korea

Address all correspondence and requests for reprints to: Marco Conti, Division of Reproductive Biology, Department of Obstetrics and Gynecology, Stanford University School of Medicine, 300 Pasteur Drive, Stanford, California 94305-5317. E-mail: marco.conti{at}stanford.edu.

Feedback regulations are integral components of the cAMP signaling required for most cellular processes, including gene expression and cell differentiation. Here, we provide evidence that one of these feedback regulations involving the cyclic nucleotide phosphodiesterase PDE4D plays a critical role in cAMP signaling during the differentiation of granulosa cells of the ovarian follicle. Gonadotropins induce PDE4D mRNA and increase the cAMP hydrolyzing activity in granulosa cells, demonstrating that a feedback regulation of cAMP is operating in granulosa cells in vivo. Inactivation of the PDE4D by homologous recombination is associated with an altered pattern of cAMP accumulation induced by the gonadotropin LH/human chorionic gonadotropin (hCG), impaired female fertility, and a markedly decreased ovulation rate. In spite of a disruption of the cAMP response, LH/hCG induced P450 side chain cleavage expression and steroidogenesis in a manner similar to wild-type controls. Morphological examination of the ovary of PDE4D-/- mice indicated luteinization of antral follicles with entrapped oocytes. Consistent with the morphological finding of unruptured follicles, LH/hCG induction of genes involved in ovulation, including cyclooxygenase-2, progesterone receptor, and the downstream genes, is markedly decreased in the PDE4D-/- ovaries. These data demonstrate that PDE4D regulation plays a critical role in gonadotropin mechanism of action and suggest that the intensity and duration of the cAMP signal defines the pattern of gene expression during the differentiation of granulosa cells.




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