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Imaging Analysis of Subcellular Correlation of Androgen Receptor and Estrogen Receptor in Single Living Cells Using Green Fluorescent Protein Color Variants

Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, Kyoto 602-8566, Japan

Address all correspondence and requests for reprints to: Mitsuhiro Kawata, Department of Anatomy and Neurobiology, Kyoto Prefectural University of Medicine, Kawaramachi Hirokoji, Kamigyo-ku, Kyoto 602-8566, Japan. E-mail: mkawata{at}basic.kpu-m.ac.jp.

Androgen and estrogen act not only in a sex-specific manner but also interactively and synergistically. In the present study, to examine the possible interaction between androgen receptor (AR) and estrogen receptor- (ER), we investigated the subcellular dynamics of AR and ER fused with green fluorescent protein color variants in single living cells using time-lapse microscopy and the technique of fluorescence recovery after photobleaching. AR and ER showed punctate colocalization in the nucleus with estrogen, but not androgen. N-terminal AR deletion mutant did not form a nuclear punctate pattern with either androgen or estrogen. In the presence of AR, but not ER, N-terminal AR deletion mutant formed a punctate nuclear pattern with androgen. AR had different mobility depending on the ligand and the presence of ER. On the other hand, AR had little effect on the stability of ER. ER mutant that does not bind coactivators did not alter the mobility of AR. Taken together, using an imaging technique, we clarified that possible homo/hetero dimerization between AR and ER could be attributed to androgen-estrogen interaction in living cells.

NURSA Molecule Pages Link:

Nuclear Receptors:   ERα  |  AR

Ligands:   17β-Estradiol

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