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Insulin-Like Growth Factor II Messenger Ribonucleic Acids are Synthesized in the Choroid Plexus of the Rat Brain

Curriculum in Neurobiology, Department of Pediatrics, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27514
Department of Physiology, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27514

Address requests for reprints to: P. Kay Lund, Department of Physiology, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514.

Abstract

Previous studies demonstrating the presence of immunoreactive insulin-like growth factors (IGFs) and their receptors in the brain suggest a role of the IGFs in the central nervous system. IGF-II has been implicated as the predominant IGF in brain of mature animals based on studies of immunoreactive peptide and of IGF-II mRNAs. To obtain information about the sites of synthesis of IGF-IIin adult rat brain, a ³²P-labeled 31 base long synthetic oligodeoxyribonucleotide complementary in sequence to trailer peptide coding sequences in rat IGF-II mRNA (IGF-II 31 mer) was hybridized with coronal sections of fixed rat brain. The IGF-II 31 mer showed specific hybridization with the choroid plexus throughout rat brain, whereas in other brain regions, structures or cells, hybridization was not discernibly above background. These findings suggest that the choroid plexus is a primary site of synthesis of IGF-II, a probable source of IGF-II in cerebrospinal fluid, and a potential source of IGF-II for actions on target cells within the adult rat brain.

FOOTNOTES

This work was supported in part by NIH Grants AM-1022 (to J.J.V.W.), Career Research Award AM-14115 (to J.J.V.W.), and NS-23804 (to P.K.L.).

Received for publication October 15, 1987. Accepted for publication November 1, 1987.

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