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The Laboratories for Reproductive Biology, Department of Pediatrics and Physiology, University of North Carolina at Chapel Hill Chapel Hill, North Carolina 27514
Address requests for reprints to: Marco Conti, The Laboratories for Reproductive Biology, C.B.7500, MacNider Building, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27599.
Abstract
The expression of the protooncogene c-fos has been associated with the transduction of cell surface stimuli into changes in nuclear function. To evaluate the possibility that this protooncogene plays a role in the gonadotropin-dependent gene regulation, the effect of FSH on the expression of c-fos was studied in primary Sertoli cell cultures. Sertoli cells were stimulated for different time intervals with FSH and c-fos mRNA levels measured by Northern RNA blot analysis. FSH treatment increased c-fos mRNA transiently with a maximal stimulation reached in 1 h. The level of c-fos mRNA returned to basal level within 4–6 h. The induction of c-fos mRNA was dependent on the concentration of FSH used with an ED50 of 3–5 ng/ml ovine FSH-16. A similar increase in c-fos expression was induced with highly purified hFSH. The c-fos mRNA was also elevated after treatment of the Sertoli cell with (Bu)2cAMP and forskolin. (Bu)2cAMP treatment led to a sustained induction of c-fos mRNA, with increased mRNA levels being maintained after 12 h. The FSH-dependent induction of c-fosmRNA was still present in cells treated for 3 h with cycloheximide, but it was greatly reduced by actinomycin D pretreatment. These data indicate that FSH induces a transient expression of c-fos in cultured Sertoli cells. This induction is probably mediated by cAMP and likely involves an increased transcription of the c-fos gene. Early expression of this gene might be an intermediate step required for gonadotropin-dependent regulation of expression of other genes.
FOOTNOTES
This research was supported by U.S. Public Health Service Grants HD-21744 (to D.R.J., HD-20788 (to M.C), 5-P30-HD-18968, and HD-04466 (to F.S.F.) and a grant from the Andrew W. Mellon Foundation.
* Supported by Training Grant T32-HD07315 from NIH.
Received for publication September 18, 1987. Accepted for publication October 24, 1987.
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