This Article Full Text (PDF) Submit a related Letter to the Editor Alert me when this article is cited Alert me when eLetters are posted Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Levy, F. O. Articles by Jahnsen, T. Search for Related Content PubMed PubMed Citation Articles by Levy, F. O. Articles by Jahnsen, T.

Molecular Cloning, Complementary Deoxyribonucleic Acid Structure and Predicted Full-Length Amino Acid Sequence of the Hormone-lnducible Regulatory Subunit of 3'-5'-Cyclic Adenosine Monophosphate-Dependent Protein Kinase from Human Testis

Institute of Medical Biochemistry, University of Oslo N-0317 Oslo 3, Norway
Institute of Pathology, University of Oslo Rikshospitalet N-0027 Oslo 1, Norway

Address requests for reprints to: Finn Olav Levy, Institute of Medical Biochemistry, University of Oslo, P.O. Box 1112 Blindem, N-0317 Oslo 3, Norway.

Abstract

In this study, we report the isolation and characterization of a full-length cDNA clone for the hormone-inducible regulatory subunit RII_β (formerly called RII₅₁) of type II cAMP-dependent protein kinase from a human testis cDNA library. The cloned cDNA demonstrated tissue-specific expression of RII_β mRNA in human tissues, with the highest mRNA levels in testis and ovary.

The isolated human cDNA clone was 3.3 kilobases (kb) in length and contained 166 base pairs (bp) of G/C-rich 5'-noncoding sequence, an open reading frame of 1254 bp and an A/T-rich 3'-nontranslated region containing 1836 bp followed by an 89 nucleotide long poly(A)-tail. The predicted protein contains 418 amino acids including the start methionine, and the estimated mol wt of human RII_β is 53,856. The nucleotide sequence within the open reading frame and the predicted amino acid sequence of human RII_β are highly conserved compared with partial rat RII_β sequences, displaying 91% and 97% similarity, respectively.

Codon preference analysis of the cloned cDNA sequence indicated that the two cAMP-binding domains and the hinge region are highly conserved through evolution, whereas the dimerization domain displayed a codon preference pattern indicative of appearance at a later stage of evolution.

The isolated human cDNA detected an FSH- and cAMP-inducible mRNA of 3.2 kb in rat Sertoli cells, thus confirming that the cloned cDNA represents the hormone-inducible regulatory subunit of cAMP-dependent protein kinase.

This is the first report documenting the isolation of a full-length cDNA clone for the RII_β, of cAMP-dependent protein kinase.

FOOTNOTES

This work was supported by the Norwegian Society for Fighting Cancer, The Norwegian Research Council for Science and the Humanities, The Norwegian Cancer Society, Astri and Birger Torsteds Grant, Anders Jahres Foundation for the Promotion of Science, and Grethe Harbitz' Grant.

Received for publication June 16, 1988. Revision received August 23, 1988. Accepted for publication August 23, 1988.

This article has been cited by other articles:

				H. Wang, M. Li, W. Lin, W. Wang, Z. Zhang, E. R. Rayburn, J. Lu, D. Chen, X. Yue, F. Shen, et al. Extracellular Activity of Cyclic AMP-Dependent Protein Kinase as a Biomarker for Human Cancer Detection: Distribution Characteristics in a Normal Population and Cancer Patients Cancer Epidemiol. Biomarkers Prev., April 1, 2007; 16(4): 789 - 795. [Abstract] [Full Text] [PDF]

				J. L. Busch, E. P. Bessay, S. H. Francis, and J. D. Corbin A Conserved Serine Juxtaposed to the Pseudosubstrate Site of Type I cGMP-dependent Protein Kinase Contributes Strongly to Autoinhibition and Lower cGMP Affinity J. Biol. Chem., September 6, 2002; 277(37): 34048 - 34054. [Abstract] [Full Text] [PDF]

				N. Mishra, I. U. Khan, G. C. Tsokos, and G. M. Kammer Association of Deficient Type II Protein Kinase A Activity with Aberrant Nuclear Translocation of the RII{beta} Subunit in Systemic Lupus Erythematosus T Lymphocytes J. Immunol., September 1, 2000; 165(5): 2830 - 2840. [Abstract] [Full Text] [PDF]

				Y. S. Cho, Y. G. Park, Y. N. Lee, M.-K. Kim, S. Bates, L. Tan, and Y. S. Cho-Chung Extracellular protein kinase A as a cancer biomarker: Its expression by tumor cells and reversal by a myristate-lacking Calpha and RIIbeta subunit overexpression PNAS, January 18, 2000; 97(2): 835 - 840. [Abstract] [Full Text] [PDF]

				D. Laxminarayana, I. U. Khan, N. Mishra, I. Olorenshaw, K. Tasken, and G. M. Kammer Diminished Levels of Protein Kinase A RI{alpha} and RI{beta} Transcripts and Proteins in Systemic Lupus Erythematosus T Lymphocytes J. Immunol., May 1, 1999; 162(9): 5639 - 5648. [Abstract] [Full Text] [PDF]

				Z. Qi, C.-M. Hao, K. Salter, R. Redha, and M. D. Breyer Type II cAMP-dependent protein kinase regulates electrogenic ion transport in rabbit collecting duct Am J Physiol Renal Physiol, April 1, 1999; 276(4): F622 - F628. [Abstract] [Full Text] [PDF]

				G Keryer, E Alsat, K Tasken, and D Evain-Brion Cyclic AMP-dependent protein kinases and human trophoblast cell differentiation in vitro J. Cell Sci., January 4, 1998; 111(7): 995 - 1004. [Abstract] [PDF]

				R. Telgmann, E. Maronde, K. Tasken, and B. Gellersen Activated Protein Kinase A Is Required for Differentiation-Dependent Transcription of the Decidual Prolactin Gene in Human Endometrial Stromal Cells Endocrinology, March 1, 1997; 138(3): 929 - 937. [Abstract] [Full Text] [PDF]

				S. H. Francis, J. A. Smith, J. L. Colbran, K. Grimes, K. A. Walsh, S. Kumar, and J. D. Corbin Arginine 75in the Pseudosubstrate Sequence of Type Ibeta cGMPdependent Protein Kinase Is Critical for Autoinhibition, Although Autophosphorylated Serine 63Is Outside This Sequence J. Biol. Chem., August 23, 1996; 271(34): 20748 - 20755. [Abstract] [Full Text] [PDF]

				Y. Li and C. S. Rubin Mutagenesis of the Regulatory Subunit (RIIbeta) of cAMP-dependent Protein Kinase IIbeta Reveals Hydrophobic Amino Acids That Are Essential for RIIbeta Dimerization and/or Anchoring RIIbeta to the Cytoskeleton J. Biol. Chem., January 27, 1995; 270(4): 1935 - 1944. [Abstract] [Full Text] [PDF]