This Article Full Text Full Text (PDF) All Versions of this Article: 20/3/483    most recent Author Manuscript (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager NURSA Molecule Pages Link Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Liu, Y. Articles by Yen, P. M. Search for Related Content PubMed PubMed Citation Articles by Liu, Y. Articles by Yen, P. M. Pubmed/NCBI databases Compound via MeSH Substance via MeSH Hazardous Substances DB LIOTHYRONINE

Thyroid Hormone-Regulated Target Genes Have Distinct Patterns of Coactivator Recruitment and Histone Acetylation

Department of Medicine, Johns Hopkins Bayview Medical Center (P.M.Y., X.X.), Johns Hopkins University, Baltimore, Maryland 21224; Molecular Regulation and Neuroendocrinology Section, Clinical Endocrinology Branch, National Institute of Diabetes and Digestive and Kidney Diseases, National Institutes of Health (Y.L.), Bethesda, Maryland 20892; and Department of Physiology and Biophysics, University of Medicine and Dentistry of New Jersey-Robert Wood Johnson Medical School (J.D.F.), Piscataway, New Jersey 08854

Address all correspondence and requests for reprints to: Dr. Paul M. Yen, Endocrinology Division, Department of Medicine, Johns Hopkins Bayview Medical Center, 4940 Eastern Avenue, Room B114, Baltimore, Maryland 21224. E-mail: pyen3{at}jhmi.edu.

Thyroid hormone receptors (TRs) are ligand-regulated transcription factors that bind to thyroid hormone response elements of target genes. Upon ligand binding, they recruit coactivator complexes that increase histone acetylation and recruit RNA polymerase II (Pol II) to activate transcription. Recent studies suggest that nuclear receptors and coactivators may have temporal recruitment patterns on hormone response elements, yet little is known about the nature of the patterns at multiple endogenous target genes. We thus performed chromatin immunoprecipitation assays to investigate coactivator recruitment and histone acetylation patterns on the thyroid hormone response elements of four endogenous target genes (GH, sarcoplasmic endoplasmic reticulum calcium-adenosine triphosphatase, phosphoenolpyruvate carboxykinase, and cholesterol 7-hydroxylase) in a rat pituitary cell line that expresses TRs. We found that TRß, several associated coactivators (steroid receptor coactivator-1, glucocorticoid receptor interacting protein-1, and TR-associated protein 220), and RNA Pol II were rapidly recruited to thyroid hormone response elements as early as 15 min after T₃ addition. When the four target genes were compared, we observed differences in the types and temporal patterns of recruited coactivators and histone acetylation. Interestingly, the temporal pattern of RNA Pol II was similar for three genes studied. Our findings suggest that thyroid hormone-regulated target genes may have distinct patterns of coactivator recruitment and histone acetylation that may enable highly specific regulation.

NURSA Molecule Pages Link:

Nuclear Receptors:   TRβ

Coregulators:   TRAP220  |  SRC-1  |  GRIP1

Ligands:   Thyroid hormone

This article has been cited by other articles:

				R. D. Nerenz, M. L. Martowicz, and J. W. Pike An Enhancer 20 Kilobases Upstream of the Human Receptor Activator of Nuclear Factor-{kappa}B Ligand Gene Mediates Dominant Activation by 1,25-Dihydroxyvitamin D3 Mol. Endocrinol., May 1, 2008; 22(5): 1044 - 1056. [Abstract] [Full Text] [PDF]

				C. Garcia-G, L. Lopez-Bojorquez, J. Nunez, C. Valverde-R, and A. Orozco 3,5-Diiodothyronine in vivo maintains euthyroidal expression of type 2 iodothyronine deiodinase, growth hormone, and thyroid hormone receptor beta1 in the killifish Am J Physiol Regulatory Integrative Comp Physiol, August 1, 2007; 293(2): R877 - R883. [Abstract] [Full Text] [PDF]

				C. B. Harvey, J. H. D. Bassett, P. Maruvada, P. M. Yen, and G. R. Williams The Rat Thyroid Hormone Receptor (TR) {Delta}{beta}3 Displays Cell-, TR Isoform-, and Thyroid Hormone Response Element-Specific Actions Endocrinology, April 1, 2007; 148(4): 1764 - 1773. [Abstract] [Full Text] [PDF]

				B. D. Paul, D. R. Buchholz, L. Fu, and Y.-B. Shi SRC-p300 Coactivator Complex Is Required for Thyroid Hormone-induced Amphibian Metamorphosis J. Biol. Chem., March 9, 2007; 282(10): 7472 - 7481. [Abstract] [Full Text] [PDF]

				F. Flamant, K. Gauthier, and J. Samarut Thyroid Hormones Signaling Is Getting More Complex: STORMs Are Coming Mol. Endocrinol., February 1, 2007; 21(2): 321 - 333. [Abstract] [Full Text] [PDF]

				D.-J. Shin, M. Plateroti, J. Samarut, and T. F. Osborne Two uniquely arranged thyroid hormone response elements in the far upstream 5' flanking region confer direct thyroid hormone regulation to the murine cholesterol 7{alpha} hydroxylase gene Nucleic Acids Res., September 1, 2006; 34(14): 3853 - 3861. [Abstract] [Full Text] [PDF]

				K. Hashimoto, M. Yamada, S. Matsumoto, T. Monden, T. Satoh, and M. Mori Mouse Sterol Response Element Binding Protein-1c Gene Expression Is Negatively Regulated by Thyroid Hormone Endocrinology, September 1, 2006; 147(9): 4292 - 4302. [Abstract] [Full Text] [PDF]

				P. M. Yen Thyroid hormones and 3,5-diiodothyropropionic Acid: new keys for new locks. Endocrinology, April 1, 2006; 147(4): 1598 - 1601. [Full Text] [PDF]