This Article Full Text Full Text (PDF) All Versions of this Article: 20/6/1366    most recent Author Manuscript (PDF) Purchase Article View Shopping Cart Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Charles, M. A. Articles by Gordon, D. F. Search for Related Content PubMed PubMed Citation Articles by Charles, M. A. Articles by Gordon, D. F.

Pituitary-Specific Gata2 Knockout: Effects on Gonadotrope and Thyrotrope Function

Department of Human Genetics (M.A.C., T.L.S., K.O., S.A.C.), University of Michigan, Ann Arbor, Michigan 48109-0618; Division of Endocrinology (W.M.W., E.C.R., D.F.G.), University of Colorado, Aurora, Colorado 80045; and National Hormone and Peptide Program (A.F.P.), Harbor-UCLA Medical Center, Torrance, California 90509

Address all correspondence and requests for reprints to: Sally A. Camper, 4909 Buhl Building, East Catherine Street, University of Michigan Medical School, Ann Arbor, Michigan 48109-0618. E-mail: scamper{at}umich.edu.

GATA2 is expressed in the pituitary during development and in adult gonadotropes and thyrotropes. It is proposed to be important for gonadotrope and thyrotrope cell fate choice and for TSH production. To test this idea, we produced a pituitary-specific knockout of Gata2, designed so that the DNA-binding zinc-finger region is deleted in the presence of a pituitary-specific recombinase transgene. These mice have reduced secretion of gonadotropins basally and in response to castration challenge, although the mice are fertile. GATA2 deficiency also compromises thyrotrope function. Mutants have fewer thyrotrope cells at birth, male Gata2-deficient mice exhibit growth delay from 3–9 wk of age, and adult mutants produce less TSH in response to severe hypothyroidism after radiothyroidectomy. Therefore, Gata2 appears to be dispensable for gonadotrope and thyrotrope cell fate and maintenance, but important for optimal gonadotrope and thyrotrope function. Gata2-deficient mice exhibit elevated levels of Gata3 transcripts in the pituitary gland, suggesting that GATA3 can compensate for GATA2.

This article has been cited by other articles:

				P. E. Lapinski, J. A. Oliver, L. A. Kamen, E. D. Hughes, T. L. Saunders, and P. D. King Genetic Analysis of SH2D4A, a Novel Adapter Protein Related to T Cell-Specific Adapter and Adapter Protein in Lymphocytes of Unknown Function, Reveals a Redundant Function in T Cells J. Immunol., August 1, 2008; 181(3): 2019 - 2027. [Abstract] [Full Text] [PDF]

				Y. Shima, M. Zubair, T. Komatsu, S. Oka, C. Yokoyama, T. Tachibana, T. A. Hjalt, J. Drouin, and K.-i. Morohashi Pituitary Homeobox 2 Regulates Adrenal4 Binding Protein/Steroidogenic Factor-1 Gene Transcription in the Pituitary Gonadotrope through Interaction with the Intronic Enhancer Mol. Endocrinol., July 1, 2008; 22(7): 1633 - 1646. [Abstract] [Full Text] [PDF]

				R. S. Viger, S. M. Guittot, M. Anttonen, D. B. Wilson, and M. Heikinheimo Role of the GATA Family of Transcription Factors in Endocrine Development, Function, and Disease Mol. Endocrinol., April 1, 2008; 22(4): 781 - 798. [Abstract] [Full Text] [PDF]

				X. Zhu, A. S. Gleiberman, and M. G. Rosenfeld Molecular Physiology of Pituitary Development: Signaling and Transcriptional Networks Physiol Rev, July 1, 2007; 87(3): 933 - 963. [Abstract] [Full Text] [PDF]

				S.-I. Kim, S. J. Bultman, H. Jing, G. A. Blobel, and E. H. Bresnick Dissecting Molecular Steps in Chromatin Domain Activation during Hematopoietic Differentiation Mol. Cell. Biol., June 15, 2007; 27(12): 4551 - 4565. [Abstract] [Full Text] [PDF]

				A. Kita, I. Imayoshi, M. Hojo, M. Kitagawa, H. Kokubu, R. Ohsawa, T. Ohtsuka, R. Kageyama, and N. Hashimoto Hes1 and Hes5 Control the Progenitor Pool, Intermediate Lobe Specification, and Posterior Lobe Formation in the Pituitary Development Mol. Endocrinol., June 1, 2007; 21(6): 1458 - 1466. [Abstract] [Full Text] [PDF]

				A. Matsushita, S. Sasaki, Y. Kashiwabara, K. Nagayama, K. Ohba, H. Iwaki, H. Misawa, K. Ishizuka, and H. Nakamura Essential Role of GATA2 in the Negative Regulation of Thyrotropin {beta} Gene by Thyroid Hormone and Its Receptors Mol. Endocrinol., April 1, 2007; 21(4): 865 - 884. [Abstract] [Full Text] [PDF]

				B. S. Ellsworth, N. Egashira, J. L. Haller, D. L. Butts, J. Cocquet, C. M. Clay, R. Y. Osamura, and S. A. Camper FOXL2 in the Pituitary: Molecular, Genetic, and Developmental Analysis Mol. Endocrinol., November 1, 2006; 20(11): 2796 - 2805. [Abstract] [Full Text] [PDF]

				L. T. Raetzman, B. S. Wheeler, S. A. Ross, P. Q. Thomas, and S. A. Camper Persistent Expression of Notch2 Delays Gonadotrope Differentiation Mol. Endocrinol., November 1, 2006; 20(11): 2898 - 2908. [Abstract] [Full Text] [PDF]