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Cell Proliferation and Vascularization in Mouse Models of Pituitary Hormone Deficiency

Graduate Program in Cellular and Molecular Biology (R.D.W., S.A.C.), and Department of Human Genetics (B.M.S., L.T.R., S.A.C.), University of Michigan, Ann Arbor, Michigan 48109-0618

Address all correspondence and requests for reprints to: Sally A. Camper, Graduate Program in Cellular and Molecular Biology, Department of Human Genetics, 4909 Buhl Building, East Catherine Street, Ann Arbor, Michigan 48109-0618. E-mail: scamper{at}umich.edu.

Mutations in the transcription factors PIT1 (pituitary transcription factor 1) and PROP1 (prophet of Pit1) lead to pituitary hormone deficiency and hypopituitarism in mice and humans. To determine the basis for this, we performed histological analysis of Pit1- and Prop1-deficient dwarf mouse pituitaries throughout fetal and postnatal development. Pit1-deficient mice first exhibit pituitary hypoplasia after birth, primarily caused by reduced cell proliferation, although there is some apoptosis. To determine whether altered development of the vascular system contributes to hypopituitarism, we examined vascularization from embryonic d 14.5 and throughout development. No obvious differences in vascularization are evident in developing Pit1-deficient pituitaries. In contrast, the Prop1-deficient mouse pituitaries are poorly vascularized and dysmorphic, with a striking elevation in apoptosis. At postnatal d 11, apoptosis-independent caspase-3 activation occurs in thyrotropes and somatotropes of normal but not mutant pituitaries. This suggests that Prop1 and/or Pit1 may be necessary for caspase-3 expression. These studies provide further insight as to the mechanisms of Prop1 and Pit1 action in mice.

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