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Mash1 Is Required for Generic and Subtype Differentiation of Hypothalamic Neuroendocrine Cells

Divisions of Developmental Neurobiology (D.E.G.M., M.P., S.C., S.-L.A.) and Molecular Neurobiology (F.G.), Medical Research Council, The National Institute of Medical Research, London NW7 1AA, United Kingdom

Address all correspondence and requests for reprints to: Siew-Lan Ang, Division of Developmental Neurobiology, Medical Research Council, The National Institute of Medical Research, Mill Hill, London NW7 1AA, United Kingdom. E-mail: sang{at}nimr.mrc.ac.uk.

The neuroendocrine hypothalamus regulates a number of critical biological processes and underlies a range of diseases from growth failure to obesity. Although the elucidation of hypothalamic function has progressed well, knowledge of hypothalamic development is poor. In particular, little is known about the processes underlying the neurogenesis and specification of neurons of the ventral nuclei, the arcuate and ventromedial nuclei. The proneural gene Mash1 is expressed throughout the basal retrochiasmatic neuroepithelium and loss of Mash1 results in hypoplasia of both the arcuate and ventromedial nuclei. These defects are due to a failure of neurogenesis and apoptosis, a defect that can be rescued by ectopic Ngn2 under the control of the Mash1 promoter. In addition to its role in neurogenesis, analysis of Mash1^–/–, Mash1^+/–, Mash1^{KINgn2/KINgn2}, and Mash1^KINgn2/+ mice demonstrates that Mash1 is specifically required for Gsh1 expression and subsequent GHRH expression, positively regulates SF1 expression, and suppresses both tyrosine hydroxylase (TH) and neuropeptide Y (NPY) expression. Although Mash1 is not required for propiomelanocortin (POMC) expression, it is required for normal development of POMC⁺ neurons. These data demonstrate that Mash1 is both required for the generation of ventral neuroendocrine neurons as well as playing a central role in subtype specification of these neurons.

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