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PURIFICATION OF PITUITARY GONADOTROPIN FROM URINE OF NORMAL MEN^*

Mayo Clinic and Mayo Foundation Rochester, Minnesota

Human pituitary gonadotropin (HPG) from urine of normal men was purified in three successive steps. HPG was extracted from urine with kaolin, eluted with ammonium hydroxide, and precipitated with acetone. The acetone precipitate was extracted with 10 per cent ammonium acetate in 70 per cent ethanol. Impurities of this extract were removed by adsorption on diethylaminoethyl cellulose. The partial purification of HPG achieved by these three steps was of the order of 50-fold. The partially purified material had the same biologic characteristics as the initial extract, as determined by the method of biologic fingerprint and by three assay systems: the ovarian-weight method in intact rats, the augmentation reaction with chorionic gonadotropin, and the ventral-prostate method in hypophysectomized rats.

^* Aided by grant A-1738, National Institutes of Health, United States Public Health Service.

Received May 21, 1960.