This Article Full Text Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager NURSA Molecule Pages Link Request Copyright Permission Citing Articles Citing Articles via Google Scholar Google Scholar Articles by Araki, O. Articles by Cheng, S. Y. Search for Related Content PubMed PubMed Citation Articles by Araki, O. Articles by Cheng, S. Y.

Distinct Dysregulation of Lipid Metabolism by Unliganded Thyroid Hormone Receptor Isoforms

Laboratory of Molecular Biology (O.A., H.Y., X.G.Z., S.Y.C.), Center for Cancer Research, National Cancer Institute, National Institutes of Health, Bethesda, Maryland 20892; and Department of Pathology (M.C.W.), Wake Forest University, Winston-Salem, North Carolina 27157

Address all correspondence and requests for reprints to: S. Y. Cheng, Laboratory of Molecular Biology, National Cancer Institute, 37 Convent Drive, Room 5128, Bethesda, Maryland 20892-4264. E-mail: chengs{at}mail.nih.gov.

Thyroid hormone receptors (TRs) play critical roles in energy homeostasis. To understand the role of TRs in lipid homeostasis in vivo, we adopted the loss-of-function approach by creating knock-in mutant mice with targeted mutation in the TR gene (TR1PV mouse) or TRβ gene (TRβPV mouse). The PV mutation, identified in a patient with resistance to thyroid hormone, exhibits potent dominant-negative activity. Here we show that in contrast to TR1PV mouse, TRβPV mice exhibited no significant reduction in WAT but had significant increases in serum free fatty acids and total triglycerides. Moreover, the liver of TRβPV mice was markedly increased (33%) with excess lipid accumulation, but the liver mass of TR1PV mouse was decreased (23%) with paucity of lipids. These results indicate that apo-TRβ and apo-TR1 exerted distinct abnormalities in lipid metabolism. Further biochemical analyses indicate that increased lipogenic enzyme expression, activated peroxisome proliferator-activated receptor (Ppar) signaling, and decreased fatty acid β-oxidation activity contributed to the adipogenic steatosis and lipid accumulation in the liver of TRβPV mice. In contrast, the expression of lipogenic enzymes and Ppar was decreased in the liver of TR1PV mice. These results suggest that the regulation of genes critical for lipid metabolism by TRs in the liver is isoform dependent. These results indicate that apo-TRβ and apo-TR1 had different effects on lipid metabolism and that both TR isoforms contribute to the pathogenesis of lipid metabolism in hypothyroidism.

NURSA Molecule Pages Link:

Coregulators:   TRα  |  TRβ  |  PPARγ