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Complementary DNA Cloning of the Murine Transforming Growth Factor-β3 (TGFβ3) Precursor and the Comparative Expression of TGFβ3 and TGFβ1 Messenger RNA in Murine Embryos and Adult Tissues

Department of Cell Biology, Vanderbilt University School of Medicine Nashville, Tennessee 37232
Departments of Developmental Biology and Molecular Biology, Genentech, Inc. South San Francisco, Calilomia 94080

Address requests for reprints to: Dr. Duncan A. Miller, Department of Cell Biology, MCN-C2310, Vanderbilt University School of Medicine, Nashville, Tennessee 37232.

Abstract

Murine transforming growth factor-β3 (TGFβ3) cDNAs were isolated from a TGFβ2-induced AKR-2B cDNA library. The composite cDNA sequence is 2894 nucleotides long, including 610-nucleotide and 1054-nucleotide 5' and 3' untranslated sequences, respectively. The murine TGFβ3-coding region is 1230 nucleotides in length and encodes a precursor protein of 410 amino acids, with a 96% peptide sequence identity with the human TGFβ3 precursor. Examination of TGFβ1 and TGFβ3 mRNA levels in adult murine tissues showed that TGFβ1 mRNA expression is predominant in spleen, lung, and placenta. In contrast, TGFβ3 RNA was present in substantial amounts in brain, heart, adipose tissue, and testis. TGFβ3 mRNA is also observed in adult mouse lung and placenta. Both TGFβ1 and TGFβ3 RNAs were present in all stages of mouse fetal development studied from 10.5–17.5 days postcoitum, with higher levels observed in the latter stages. The differential expression of these TGFβ genes suggests that the various TGFβ species may have distinct physiological roles in vivo.

FOOTNOTES

This work was supported in part by NIH Grant CA-42572.

Received for publication August 14, 1989. Revision received September 7, 1989. Accepted for publication September 7, 1989.

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