help button home button Endocrine Society Molecular Endocrinology ENDO 08 Sessions Library
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Molecular Endocrinology Vol. 3, No. 3 509-517
doi:10.1210/mend-3-3-509
Copyright © 1989 by the Endocrine Society.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Yee, D.
Right arrow Articles by Rosen, N.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Yee, D.
Right arrow Articles by Rosen, N.

Analysis of Insulin-Like Growth Factor I Gene Expression in Malignancy: Evidence for a Paracrine Role in Human Breast Cancer

Douglas Yee, Soonmyoung Paik, Gail S. Lebovic, Rachel R. Marcus, Roberto E. Favoni, Kevin J. Cullen, Marc E. Lippman and Neal Rosen

Medical Breast Cancer Section, National Cancer Institute Bethesda, Maryland 20892

Address requests for reprints to: Dr. Douglas Yee, Lombardi Cancer Research Center, Georgetown University Medical Center, Washington, D. C. 20007. This is the current address for all authors.

Abstract

Insulin-like growth factor I (IGF-I) activity has been reported to be produced by several human cancers. Identification of RNAs transcribed from the IGF-I gene has been complicated by the detection of multiple hybridizing bands on Northern analysis. To determine if any of these RNAs are transcribed from the IGF-I gene, we have used a sensitive and specific ribonuclease (RNAse) protection assay for IGF-I. We have also studied the breast cancer tissue expression of IGF-I using in situ hybridization histochemistry. We have found no IGF-I mRNA in breast (zero of 11) or colon cancer (zero of 9) cell lines; both of these tumors have been previously reported to express IGF-I mRNA. However, three of three neuroepithelioma and one of two Ewing's sarcoma cell lines express IGF-I mRNA; therefore, in these tumors IGF-I may be an autocrine growth factor. In contrast to breast cancer cell lines, RNA extracted from breast tissues has easily detectable IGF-I mRNA. In situ hybridizations show that IGF-I mRNA is expressed in the stromal cells, and not by normal or malignant epithelial cells. These findings suggest that although IGF-I is not produced by breast epithelial cells it may function as either a paracrine stimulator of epithelial cells or an autocrine stimulator of stromal cells.

Received for publication September 20, 1988. Revision received November 14, 1988. Accepted for publication November 15, 1988.




This article has been cited by other articles:


Home page
 EndocrinologyHome page
A. M. Rowzee, D. L. Ludwig, and T. L. Wood
Insulin-Like Growth Factor Type 1 Receptor and Insulin Receptor Isoform Expression and Signaling in Mammary Epithelial Cells
Endocrinology, August 1, 2009; 150(8): 3611 - 3619.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
E. Semenova, H. Koegel, S. Hasse, J. E. Klatte, E. Slonimsky, D. Bilbao, R. Paus, S. Werner, and N. Rosenthal
Overexpression of mIGF-1 in Keratinocytes Improves Wound Healing and Accelerates Hair Follicle Formation and Cycling in Mice
Am. J. Pathol., November 1, 2008; 173(5): 1295 - 1310.
[Abstract] [Full Text] [PDF]

Home page
 Am. J. Pathol.Home page
K. K. de Ostrovich, I. Lambertz, J. K. L. Colby, J. Tian, J. E. Rundhaug, D. Johnston, C. J. Conti, J. DiGiovanni, and R. Fuchs-Young
Paracrine Overexpression of Insulin-Like Growth Factor-1 Enhances Mammary Tumorigenesis in Vivo
Am. J. Pathol., September 1, 2008; 173(3): 824 - 834.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Prevention ResearchHome page
T. Moore, L. Beltran, S. Carbajal, S. Strom, J. Traag, S. D. Hursting, and J. DiGiovanni
Dietary Energy Balance Modulates Signaling through the Akt/Mammalian Target of Rapamycin Pathways in Multiple Epithelial Tissues
Cancer Prevention Research, June 1, 2008; 1(1): 65 - 76.
[Abstract] [Full Text] [PDF]

Home page
 Clin. Cancer Res.Home page
L. N. Harris, F. You, S. J. Schnitt, A. Witkiewicz, X. Lu, D. Sgroi, P. D. Ryan, S. E. Come, H. J. Burstein, B.-A. Lesnikoski, et al.
Predictors of Resistance to Preoperative Trastuzumab and Vinorelbine for HER2-Positive Early Breast Cancer
Clin. Cancer Res., February 15, 2007; 13(4): 1198 - 1207.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
A. A. Samani, S. Yakar, D. LeRoith, and P. Brodt
The Role of the IGF System in Cancer Growth and Metastasis: Overview and Recent Insights
Endocr. Rev., February 1, 2007; 28(1): 20 - 47.
[Abstract] [Full Text] [PDF]

Home page
 J EndocrinolHome page
S. Maor, D. Mayer, R. I Yarden, A. V Lee, R. Sarfstein, H. Werner, and M. Z Papa
Estrogen receptor regulates insulin-like growth factor-I receptor gene expression in breast tumor cells: involvement of transcription factor Sp1
J. Endocrinol., December 1, 2006; 191(3): 605 - 612.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. M. Carboni, A. V. Lee, D. L. Hadsell, B. R. Rowley, F. Y. Lee, D. K. Bol, A. E. Camuso, M. Gottardis, A. F. Greer, C. P. Ho, et al.
Tumor Development by Transgenic Expression of a Constitutively Active Insulin-Like Growth Factor I Receptor
Cancer Res., May 1, 2005; 65(9): 3781 - 3787.
[Abstract] [Full Text] [PDF]

Home page
 J. Clin. Pathol.Home page
L-T Busund, E Richardsen, R Busund, T Ukkonen, T Bjornsen, C Busch, and H Stalsberg
Significant expression of IGFBP2 in breast cancer compared with benign lesions
J. Clin. Pathol., April 1, 2005; 58(4): 361 - 366.
[Abstract] [Full Text] [PDF]

Home page
 Mol. Biol. CellHome page
A. M. Valverde, C. Mur, M. Brownlee, and M. Benito
Susceptibility to Apoptosis in Insulin-like Growth Factor-I Receptor-deficient Brown Adipocytes
Mol. Biol. Cell, November 1, 2004; 15(11): 5101 - 5117.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
H.-S. Kim, A. R. Ingermann, J. Tsubaki, S. M. Twigg, G. E. Walker, and Y. Oh
Insulin-Like Growth Factor-Binding Protein 3 Induces Caspase-Dependent Apoptosis through a Death Receptor-Mediated Pathway in MCF-7 Human Breast Cancer Cells
Cancer Res., March 15, 2004; 64(6): 2229 - 2237.
[Abstract] [Full Text] [PDF]

Home page
 Endocr. Rev.Home page
A. Colao, D. Ferone, P. Marzullo, and G. Lombardi
Systemic Complications of Acromegaly: Epidemiology, Pathogenesis, and Management
Endocr. Rev., February 1, 2004; 25(1): 102 - 152.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Epidemiol. Biomarkers Prev.Home page
M. P. Cleary, M. K. Jacobson, F. C. Phillips, S. C. Getzin, J. P. Grande, and N. J. Maihle
Weight-Cycling Decreases Incidence and Increases Latency of Mammary Tumors to a Greater Extent Than Does Chronic Caloric Restriction in Mouse Mammary Tumor Virus- Transforming Growth Factor-{alpha} Female Mice
Cancer Epidemiol. Biomarkers Prev., September 1, 2002; 11(9): 836 - 843.
[Abstract] [Full Text] [PDF]

Home page
 JCOHome page
R. T. Chlebowski, E. Aiello, and A. McTiernan
Weight Loss in Breast Cancer Patient Management
J. Clin. Oncol., February 15, 2002; 20(4): 1128 - 1143.
[Abstract] [Full Text] [PDF]

Home page
 Pharmacol. Rev.Home page
R. Clarke, F. Leonessa, J. N. Welch, and T. C. Skaar
Cellular and Molecular Pharmacology of Antiestrogen Action and Resistance
Pharmacol. Rev., March 1, 2001; 53(1): 25 - 72.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
Y. Wang, S. W. Hayward, A. A. Donjacour, P. Young, T. Jacks, J. Sage, R. Dahiya, R. D. Cardiff, M. L. Day, and G. R. Cunha
Sex Hormone-induced Carcinogenesis in Rb-deficient Prostate Tissue
Cancer Res., November 1, 2000; 60(21): 6008 - 6017.
[Abstract] [Full Text]

Home page
 Endocr. Rev.Home page
H. M. Khandwala, I. E. McCutcheon, A. Flyvbjerg, and K. E. Friend
The Effects of Insulin-Like Growth Factors on Tumorigenesis and Neoplastic Growth
Endocr. Rev., June 1, 2000; 21(3): 215 - 244.
[Abstract] [Full Text]

Home page
 Pharmacol. Rev.Home page
R. E. Favoni and A. De Cupis
The Role of Polypeptide Growth Factors in Human Carcinomas: New Targets for a Novel Pharmacological Approach
Pharmacol. Rev., June 1, 2000; 52(2): 179 - 206.
[Abstract] [Full Text] [PDF]

Home page
 Cancer Res.Home page
J. DiGiovanni, D. K. Bol, E. Wilker, L. Beltrán, S. Carbajal, S. Moats, A. Ramirez, J. Jorcano, and K. Kiguchi
Constitutive Expression of Insulin-like Growth Factor-1 in Epidermal Basal Cells of Transgenic Mice Leads to Spontaneous Tumor Promotion
Cancer Res., March 1, 2000; 60(6): 1561 - 1570.
[Abstract] [Full Text]

Home page
 J. Pharmacol. Exp. Ther.Home page
A. Vogt, A. S. Wang, C. S. Johnson, J. P. Fabisiak, P. Wipf, and J. S. Lazo
In Vivo Antitumor Activity and Induction of Insulin-Like Growth Factor-1-Resistant Apoptosis by SC-alpha alpha delta 9
J. Pharmacol. Exp. Ther., February 1, 2000; 292(2): 530 - 537.
[Abstract] [Full Text]

Home page
 JCBHome page
N. Farrelly, Y.-J. Lee, J. Oliver, C. Dive, and C. H. Streuli
Extracellular Matrix Regulates Apoptosis in Mammary Epithelium through a Control on Insulin Signaling
J. Cell Biol., March 22, 1999; 144(6): 1337 - 1348.
[Abstract] [Full Text] [PDF]

Home page
 JAMAHome page
J. A. O'Shaughnessy
Chemoprevention of Breast Cancer
JAMA, May 1, 1996; 275(17): 1349 - 1353.
[Abstract] [PDF]

Home page
 J. Biol. Chem.Home page
A. J. Butt, S. M. Firth, M. A. King, and R. C. Baxter
Insulin-like Growth Factor-binding Protein-3 Modulates Expression of Bax and Bcl-2 and Potentiates p53-independent Radiation-induced Apoptosis in Human Breast Cancer Cells
J. Biol. Chem., December 8, 2000; 275(50): 39174 - 39181.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1989 by The Endocrine Society