| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Molecular Endocrinology, Vol 3, 588-596, Copyright © 1989 by Endocrine Society
ARTICLES |
CD Rasmussen and AR Means
Department of Cell Biology, Baylor College of Medicine, Houston, Texas 77030.
Based on studies that have examined the effect of calcium chelators on cells, it has been proposed that this cation plays a role in regulating cell proliferation. In this study a novel approach was used to indirectly examine the role of calcium in cell cycle progression. A cDNA for the Ca2+-binding protein parvalbumin has been expressed in mouse C127 cells, using a bovine papilloma virus-based expression vector. The normal role of parvalbumin is that of a calcium buffer in vertebrate fast twitch muscle, and the C127 cells do not normally express this protein. The presence of parvalbumin had several effects on the growth of C127 cells. The most striking phenotype was an increase in cell cycle duration which analysis showed was the result of an increase the length of G1 and mitosis (predominantly at prophase). Since changes in cell cycle duration typically occur as a result of changes in G1 duration, the observed increase in the length of mitosis is most unusual. The present results indicate that the previously observed increase in the rate of cell proliferation in cells with elevated calmodulin levels is not the result of a general increase in the level of cytoplasmic calcium-binding protein, but is specific to calmodulin. In addition, the results suggest that calcium regulates progression through mitosis by both calmodulin-dependent (metaphase transition) and -independent (prophase) mechanisms.
This article has been cited by other articles:
![]() |
M. A. Rodrigues, D. A. Gomes, M. F. Leite, W. Grant, L. Zhang, W. Lam, Y.-C. Cheng, A. M. Bennett, and M. H. Nathanson Nucleoplasmic Calcium Is Required for Cell Proliferation J. Biol. Chem., June 8, 2007; 282(23): 17061 - 17068. [Abstract] [Full Text] [PDF] |
||||
![]() |
T. Pusl, J. J. Wu, T. L. Zimmerman, L. Zhang, B. E. Ehrlich, M. W. Berchtold, J. B. Hoek, S. J. Karpen, M. H. Nathanson, and A. M. Bennett Epidermal Growth Factor-mediated Activation of the ETS Domain Transcription Factor Elk-1 Requires Nuclear Calcium J. Biol. Chem., July 19, 2002; 277(30): 27517 - 27527. [Abstract] [Full Text] [PDF] |
||||
![]() |
K. M. Elkins, P. Z. Gatzeva-Topalova, and D. J. Nelson Molecular dynamics study of Ca2+ binding loop variants of parvalbumin with modifications at the `gateway' position Protein Eng. Des. Sel., February 1, 2001; 14(2): 115 - 126. [Abstract] [Full Text] [PDF] |
||||
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |