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Transcriptional and Posttranscriptional Regulation of Sexually Differentiated Rat Liver Cytochrome P-450 by Growth Hormone

Department of Medical Nutrition, Karolinska Institutet, Huddinge University Hospital F69 S-141 86 Huddinge, Sweden

Address requests for reprints to: Dr Agneta Mode, Department of Medical Nutrition, Huddinge University Hospital F69, S-141 86 Huddinge, Sweden.

Abstract

The female specific cytochrome P-450_15β and the male specific cytochrome P-450₁₆ are the major constitutive P-450 forms in rat liver. Their expression is pretranslationally regulated by the sex specific plasma pattern of GH. To measure the mRNA levels of these hemoproteins in studies on their regulation and expression we set up specific solution hybridization assays with in vitro transcribed cRNA probes. An extensive tissue distribution study showed that both P-450_15β and P-450₁₆ are specifically expressed in the liver. Investigation of the developmental regulation of respective mRNA showed that the onset of sexual differentiation of P-450_15β and P-450₁₆ expression coinsides with the start of puberty. To assess the level(s) of the pretranslational GH regulation of P-450_15β and P-450₁₆ nuclear run-on transcription analysis was performed. Sex characteristic administration of GH to hypophysectomized rats indicated that GH exerts its dual effects via both transcriptional and postranscriptional mechanisms.

FOOTNOTES

This work was supported by a grant from the Swedish Medical Research Council (No 13X-2819).

Received for publication March 21, 1989. Revision received April 25, 1989. Accepted for publication April 25, 1989.

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