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Hormonal Regulation of Thyroid Peroxidase In Normal And Transformed Rat Thyroid Cells

Centro di Endocrinologia ed Oncologia Sperimentale del C.N.R., II Facoltà di Medicina e Chirurgia Napoli, Italy
Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facoltà di Medicina e Chirurgia Napoli, Italy
Istituto di Biologia, Facoltà di Medicina Udine, Italy

Address requests for reprints to: Dr. Raffaele Zarrilli, Dipartimento di Biologia e Patologia Cellulare e Molecolare, II Facolta di Medicine e Chirurgia, Via S. Pansini, 5,80131, Napoli, Italy.

Abstract

The hormonal induction of thyroid peroxidase (TPO) mRNA is studied in the functional rat thyroid cell line FRTL-5 and compared to the induction of thyroglobulin (TG) mRNA and I^– uptake. TPO and TG mRNAs are regulated by TSH and by insulin-like growth factor I (IGF-I) and/or insulin. However, while TPO is more sensitive to TSH regulation (5- to 6-fold increase vs. 2- to 3-fold increase by IGF-I), TSH and IGF-I are equally potent in increasing TG mRNA levels (3- to 4-fold). Regulation of I^– uptake appears to be different: thus TSH greatly (15-fold) increases I^– uptake, while IGF-I or insulin are completely ineffective. TPO and TG mRNAs and I^– transport display different sensitivity to transformation of rat thyroid cells. Thus, when another differentiated rat thyroid cell line, the PC cells, are transformed by human c-myc (PC myc), TPO and TG mRNAs are both present at normal levels, while I^– uptake is slightly decreased; in the PC cells transformed by polyomavirus middle-T-antigen (PC PyMLV) TPO mRNA is undetectable and I^– uptake is greatly decreased, while TG mRNA is present at normal levels. All three differentiated functions are switched off in PC cells transformed by the cooperation of c-myc and polyomavirus middle-T-antigen (PC myc + PyMLV).

Received for publication August 10, 1989. Revision received September 29, 1989. Accepted for publication September 29, 1989.

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