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Structural and Regulatory Analysis of the Male-Specific Rat Liver Cytochrome P-450 g: Repression by Continuous Growth Hormone Administration.

Department of Medical Nutrition, F60, Karolinska Institute, Huddinge University Hospital S-141 86 Huddinge, Sweden

Address requests for reprints to: Dr. Peter G. Zaphiropoulos, Karolinska Institute, Department of Medical Nutrition, F60,Huddinge University Hospital, S-141 86 Huddinge, Sweden.

Abstract

Complementary DNA clones encoding the male-specific rat liver cytochrome P-450 g have been isolated by cross-hybridization with sequences from the female-specific rat liver cytochrome P-450 15β.

Tissue distribution analysis indicates the liver as the organ with major expression of this cytochrome P-450 gene. Minimal P-450 g expression was also detected in prostate, kidney, heart, and brain. A developmental analysis reveals liver expression in the 8-week-old male and to a lesser extent in the 4-week-old male, but no detectable expression is seen in females of these ages or in 1- and 2-week-old rats from both sexes. Hypophysectomy of female rats dramatically increases hepatic expression of P-450 g, whereas continuous GH administration represses hepatic expression in male or female hypophysectomized rats. In similarity to P-450 15β and P-450 16, therefore, the cytochrome P-450 g gene in liver is GH regulated.

FOOTNOTES

^* Recipients of fellowships from the Swedish Medical Research Council.

Received for publication July 31, 1989. Revision received September 6, 1989. Accepted for publication September 20, 1989.