This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Atkinson, M. J. Articles by Kronenberg, H. M. Search for Related Content PubMed PubMed Citation Articles by Atkinson, M. J. Articles by Kronenberg, H. M.

Thymosin β⁴ is Expressed in ROS 17/2.8 Osteosarcoma Cells in a Regulated Manner

Endocrine Unit, Massachusetts General Hospital Boston, Massachusetts
Abteilung für Klinische Endokrinologie, Medizinische Hochschule Hannover Hannover, West Germany

Address requests for reprints to: Dr. M. J. Atkinson, Abteilung for Klinische, Endokrinologie, Medizinische Hochschule Hannover, D3000 Hannover 61, West Germany.

Abstract

The differential expression of mRNAs between the closely related rat osteosarcoma cell lines ROS 17/2.8 and ROS 25/1 was used to identify genes whose expression is associated with the osteoblast phenotype. Thymosin β₄ cDNA was cloned from an ROS 17/2.8 complimentary DNA library on the basis of its differential hybridization with radiolabeled cDNA prepared from ROS 17/2.8 and ROS 25/1 cells. Northern blot analysis confirmed that thymosin β₄ hitherto a putative immunodulatory hormone, was indeed differentially expressed. Steady state mRNA levels were severalfold higher in ROS 17/2.8 cells exhibiting an osteoblast-like phenotype, compared with the less osteoblast-like ROS 25/1. Thymosin β₄ transcripts were also detected in rat UMR 106 osteosarcoma cells and in intact neonatal and fetal rat calvaria. Sequence analysis of the cDNA indicated that thymosin β₄ transcripts may arise by processing at a more distal polyadenylation signal. Treatment of ROS 17/2.8 cells with dexamethasone increased, while addition of 1,25-dihydroxyvitamin D₃ decreased thymosin β₄ mRNA. The phenotype-dependent expression in the ROS cells and the response to steroid hormone suggest that thymosin β₄ expression contributes to the osteoblast phenotype.

FOOTNOTES

This work was supported by NIH Grant DK-36597.

^* Recipient of a Boehringer-lngelheim fellowship.

Received for publication July 10, 1989. Revision received August 28, 1989. Accepted for publication September 20, 1989.

This article has been cited by other articles:

				T. Takano, Y. Hasegawa, A. Miyauchi, F. Matsuzuka, H. Yoshida, K. Kuma, and N. Amino Quantitative Analysis of Thymosin Beta-10 Messenger RNA in Thyroid Carcinomas Jpn. J. Clin. Oncol., July 1, 2002; 32(7): 229 - 232. [Abstract] [Full Text] [PDF]

				D. Grant, J. Kinsella, M. Kibbey, S LaFlamme, P. Burbelo, A. Goldstein, and H. Kleinman Matrigel induces thymosin beta 4 gene in differentiating endothelial cells J. Cell Sci., January 12, 1995; 108(12): 3685 - 3694. [Abstract] [PDF]