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Glucocorticoids Regulate Peptidyl-Glycine -Amidating Monooxygenase Gene Expression in the Rat Hypothalamic Paraventricular Nucleus

Laboratoire de Neuroendocrinologie Expérimentale INSERM U. 297 Marseille, France

Address requests for reprints to: Michel Grino, M.D., Ph.D., Laboratoire de Neuroendocrinologie Expérimentale, INSERM U. 297, Faculté de Médecine Nord, boulevard P. Dramard, 13326 Marseille Cedex 15, France.

Abstract

Peptidyl-glycine -amidating monooxygenase (PAM) is a posttranslational processing enzyme which catalyzes the formation of biologically active -amidated peptides. The two major neuropeptides involved in the regulation of ACTH secretion [CRF and arginine vasopressin (AVP)], synthesized in the parvocellular part of the hypothalamic paraventricular nucleus (PVN), are amidated, and their synthesis and/or release is negatively regulated by glucocorticoids. In this study, using in situ hybridization, we have shown that PAM mRNA is abundantly expressed in the hypothalamic paraventricular and supraoptic nucleus. Surgical adrenalectomy (ADX) induced increases in PAM, CRF, and AVP mRNA in the parvocellular part of the PVN, while corticosterone treatment normalized these values. PAM and AVP gene expression were not changed in the magnocellular part of the PVN or in the supraoptic nucleus. These observations suggest that in addition to stimulation of CRF and AVP synthesis, ADX induces an increase in PAM synthesis in the PVN and, thus, support the hypothesis of increased secretion of both CRF and AVP after ADX.

Received for publication May 31, 1990. Revision received July 9, 1990. Accepted for publication July 9, 1990.

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