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Molecular Endocrinology Vol. 4, No. 2 191-195
doi:10.1210/mend-4-2-191
Copyright © 1990 by the Endocrine Society.
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Localization of the Sites of Synthesis and Action of Insulin-Like Growth Factor-I in the Rat Uterus

Aziz Ghahary, Subrata Chakrabarti and Liam J. Murphy*

Departments of Internal Medicine, Physiology and Pathology, Faculty of Medicine, University of Manitoba Winnipeg, Manitoba, Canada R3E 0W3

Address requests for reprints to: Liam J. Murphy, MB PhD,University of Manitoba, Department of Physiology, Room 435 Basic Science Building, 770 Bannatyne Ave., Winnipeg R3E 0W3.

Abstract

In this report we have used in situ hybridization to localize insulin-like growth factor-l (IGF-I) expression in the uterus and have examined the effects of exogenous IGF on 3H-methyl thymidine incorporation into DNA, in uterine sections in organ culture. IGF-I mRNA was detected in all layers of the uterus but was particularly abundant in the outer longitudinal smooth muscle layer. Although IGF-I mRNA was detectable in untreated, immature rat uteri, the abundance in each layer of the uterus was increased after 17β-estradiol (E2) administration. A similar increase was seen in uteri from ovariectomized, hypophysectomized rats treated with E2. IGF-I when added to uterine sections in organ culture had no significant effect on DNA synthesis in the absence of E2. However, a dose-dependent increase in DNA synthesis was seen in the presence of E2. The halfmaximal and maximal responses required 1 and 10 ng IGF-I, respectively. Autoradiographic localization of 3H-methyl thymidine incorporation into DNA demonstrated that the majority of the DNA synthesis occurred in the stromal layer. These findings are consistent with the hypothesis that IGF-I may function as a paracrine growth factor, mediating stromalmyometrial cell interaction.

FOOTNOTES

This research was supported by grants from MRC and the Manitoba Health Research Council.

* Recipient of a MRC Scholarship award.

Received for publication September 28, 1989. Revision received October 31, 1989. Accepted for publication October 31, 1989.




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