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Molecular Endocrinology Vol. 4, No. 2 268-275
doi:10.1210/mend-4-2-268
Copyright © 1990 by the Endocrine Society.
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Structure of Two in Tandem Human 17β-Hydroxysteroid Dehydrogenase Genes

Van Luu-The, Claude Labrie, Jacques Simard, Yves Lachance, Hui-Fen Zhao, Jacques Couët, Gilles Leblanc and Fernand Labrie

Medical Research Council Group in Molecular Endocrinology, Research Centre, Laval University Medical Centre Québec, G1V 4G2, Canada

Address requests for reprints to: Professor Fernand Labrie, MRC Group in Molecular Endocrinology, Research Centre, Laval University Medical Centre, 2705 Laurier Boulevard, Québec G1V4G2; Canada.

Abstract

Two human 17β-hydroxysteroid dehydrogenase (17β-HSD) genes (h17β-HSDI and h17β-HSDII) included in tandem within an approximately 13 kilobase pair fragment were isolated from a genomic {lambda}EMBL3 DNA library using cDNA encoding human 17β-HSD (hpE2DH216) as probe. We have determined the complete exon and intron sequences of the two genes as well as their 5' and 3'-flanking regions. Human 17β-HSDII contains six exons and five short introns for a total length of 3250 base pairs. The exon sequence of h17β-HSDII is identical to the previously reported hpE2DH216 cDNA while the overlapping nucleotide sequences of the corresponding exons and introns of h17β-HSDI and h170-HSDII show 89% homology. In addition, we have used the hpE2DH216 cDNA to demonstrate the widespread expression of 17β-HSD mRNAs in steroidogenic and peripheral target tissues. These new findings provide the basis for a better understanding of the molecular mechanisms involved in 17β-HSD deficiency and peripheral sex steroid metabolism.

Received for publication August 8, 1989. Revision received October 23, 1989. Accepted for publication October 30, 1989.




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