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Molecular Endocrinology Vol. 4, No. 2 312-320
doi:10.1210/mend-4-2-312
Copyright © 1990 by the Endocrine Society.
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The Chicken c-erbA {alpha}-Product Induces Expression of Thyroid Hormone-Responsive Genes in 3,5,3'-Triiodothyronine Receptor- Deficient Rat Hepatoma Cells

Alberto Muñoz*, Wolfgang Höppner, Jan Sap, Ged Brady, Kristina Nordström, Hans J. Seitz and Björn Vennström

Differentiation Programme, European Molecular Biology Laboratory Postfach 102209 D-6900 Heidelberg, West Germany
Universitats-Krankenhaus Eppendorf D-2000 Hamburg 20, West Germany
Department of Molecular Biology, Karolinska Institute 5–104 01 Stockholm, Sweden

Address requests for reprints to: Dr. Björn Vennstrom, Department of Molecular Biology, Karolinska Institute, Box 60400, S-104 01 Stockholm, Sweden.

Abstract

To determine the capacity of the chicken c-erbA (cTR-{alpha}) gene product in regulating expression of known thyroid hormone-responsive genes, both the cTR-{alpha} and the viral v-erbA genes were expressed in FAO cells, a rat hepatoma cell line defective for functional thyroid hormone receptors. Upon nuclear expression of the cTR-{alpha} protein the cells become responsive to thyroid hormone, as detected by expression of a number of genes (malic enzyme, phosphoenolpyruvate carboxykinase, and Na+/K+- ATPase) reported to be indirectly induced by the hormone in vivo. In addition, our data show that the c-erbA product directly activates the Moloney murine leukemia virus promoter in a ligand-dependent manner. The data show that the chicken c-erbA-a protein can modulate the expression of rat genes under either direct or indirect control by thyroid hormone.

FOOTNOTES

* Present address: Instituto de Investigaciones Biomedicas, Facultad de Medicina, Universite Autonoma, 28029 Madrid, Spain. Supported by an EMBO Long Term Fellowship during sabbatical leave from Instituto de Investigaciones Biomedicas, Spain.

Received for publication April 17, 1989. Revision received August 18, 1989. Accepted for publication October 13, 1989.






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Copyright © 1990 by The Endocrine Society