This Article Full Text (PDF) Alert me when this article is cited Alert me if a correction is posted Citation Map Services Email this article to a friend Similar articles in this journal Similar articles in PubMed Alert me to new issues of the journal Download to citation manager Request Copyright Permission Citing Articles Citing Articles via HighWire Citing Articles via Google Scholar Google Scholar Articles by Murphy, L. J. Articles by Kennedy, T. G. Search for Related Content PubMed PubMed Citation Articles by Murphy, L. J. Articles by Kennedy, T. G.

Identification and Characterization of a Rat Decidual Insulin-Like Growth Factor-Binding Protein Complementary DNA

Liam J. Murphy^*, Charita Seneviratne, Gustavo Ballejo, France Croze and Thomas G. Kennedy

Departments of Internal Medicine and Physiology, University of Manitoba Winnipeg, Manitoba, Canada
Departments of Obstetrics and Gynecology and Physiology, University of Western Ontario London, Ontario, Canada

Address requests for reprints to: Dr. Liam J. Murphy, Department of Physiology, University of Manitoba, Room 435, Basic Medical Sciences Building, 770 Bannatyne Avenue, Winnipeg, Manitoba, Canada R3E 0W3.

Abstract

A partial cDNA which encodes the rat homolog of human placental protein-12, the low mol wt insulinlike growth factor-binding protein (IGFBP-1), has been isolated from a rat decidual cDNA library using low stringency hybridization with a human IGFBP-1 cDNA probe. The incomplete cDNA obtained from this library was used to screen a rat liver cDNA library from which a full-length cDNA was obtained. The predicted amino acid sequence of rat IGFBP-1 showed 66%, 29%, and 34% sequence identity with the human IGFBP-1, the human GH-dependent binding protein IGFBP-3, and rat IGFBP-2, the BP secreted by buffalo rat liver cells, respectively. The rat IGFBP-1 cDNA hybridized with a 1.6-kilobase transcript which was easily detected in uterine RNA from the pseudopregnant rat and RNA from the liver and kidney of adult rats. A low level of expression was apparent in the brain and the diestrous uterus. Of the tissues examined the order of abundance of IGFBP-1 mRNA was deciduoma tissue liver >> kidney >> uterus > brain. The 1.6-kb mRNA was more abundant in RNA from neonatal rat liver than in that from maternal liver, but was not detected in total RNA (50 µg) from other neonatal rat tissues (kidney, lung, brain, and heart). Under stringent conditions, rat IGFBP-1 did not hybridize with RNA from the BRL-3A rat liver cell line. In food-deprived rats, hepatic IGFBP-1 mRNA was increased 10.0 ± 2.2- fold compared to that in control rats. Ligand blotting with [¹²⁵I]IGF-I was used to detect the BP in conditioned medium from COS-1 cells transfected with rat IGFBP-1 cDNA driven by the SV40 promoter. From these studies we conclude that the rat IGFBP-1 is expressed in some nonhepatic tissues, is more highly expressed in the neonatal rat, and is upregulated after food deprivation. The availability of a rat cDNA probe should provide a useful tool to examine the regulation of IGFBP-1 expression.

FOOTNOTES

This research was supported by grants from the Medical Research Council, the Manitoba Health Research Council, and the Canadian Diabetes Association.

^* Recipient of a Medical Research Council Scholarship award.

Recipient of a Rockefeller Biotechnology Career Fellowship (RF-86011 -35). Permanent address: Faculdade De Medicina De Ribeirao Preto, Universidade De Sao Paulo, Sao Paulo, Brazil.

Received for publication October 3, 1989. Revision received November 14, 1989. Accepted for publication November 16, 1989.

This article has been cited by other articles:

				Y. Ning, A. G. P. Schuller, C. A. Conover, and J. E. Pintar Insulin-Like Growth Factor (IGF) Binding Protein-4 Is Both a Positive and Negative Regulator of IGF Activity in Vivo Mol. Endocrinol., May 1, 2008; 22(5): 1213 - 1225. [Abstract] [Full Text] [PDF]

				Y. Ning, B. Hoang, A. G. P. Schuller, T. P. Cominski, M.-S. Hsu, T. L. Wood, and J. E. Pintar Delayed Mammary Gland Involution in Mice with Mutation of the Insulin-Like Growth Factor Binding Protein 5 Gene Endocrinology, May 1, 2007; 148(5): 2138 - 2147. [Abstract] [Full Text] [PDF]

				T. G. Anthony, J. C. Anthony, M. S. Lewitt, S. M. Donovan, and D. K. Layman Time course changes in IGFBP-1 after treadmill exercise and postexercise food intake in rats Am J Physiol Endocrinol Metab, April 1, 2001; 280(4): E650 - E656. [Abstract] [Full Text] [PDF]

				A. Takenaka, N. Oki, S.-I. Takahashi, and T. Noguchi Dietary Restriction of Single Essential Amino Acids Reduces Plasma Insulin-Like Growth Factor-I (IGF-I) but Does Not Affect Plasma IGF-Binding Protein-1 in Rats J. Nutr., December 1, 2000; 130(12): 2910 - 2914. [Abstract] [Full Text] [PDF]

				A. K. Ghoshal, Z. Xu, G. A. Wood, and M. C. Archer Induction of Hepatic Insulin-Like Growth Factor Binding Protein-1 (IGFBP-1) in Rats by Dietary n-6 Polyunsaturated Fatty Acids Experimental Biology and Medicine, November 1, 2000; 225(2): 128 - 135. [Abstract] [Full Text]

				E. M. Zimmermann, L. Li, E. C. Hoyt, J. B. Pucilowska, S. Lichtman, and P. K. Lund Cell-specific localization of insulin-like growth factor binding protein mRNAs in rat liver Am J Physiol Gastrointest Liver Physiol, March 1, 2000; 278(3): G447 - G457. [Abstract] [Full Text] [PDF]

				J. Frystyk, P. J. D. Delhanty, C. Skjarbak, and R. C. Baxter Changes in the circulating IGF system during short-term fasting and refeeding in rats Am J Physiol Endocrinol Metab, August 1, 1999; 277(2): E245 - E252. [Abstract] [Full Text] [PDF]

				Y. Gu, W.S. Branham, D.M. Sheehan, P.J. Webb, C.L. Moland, and R.D. Streck Tissue-Specific Expression of Messenger Ribonucleic Acids for Insulin-Like Growth Factors and Insulin-Like Growth Factor-Binding Proteins during Perinatal Development of the Rat Uterus Biol Reprod, May 1, 1999; 60(5): 1172 - 1182. [Abstract] [Full Text]

				T. A. Gautsch, S. M. Kandl, S. M. Donovan, and D. K. Layman Growth Hormone Promotes Somatic and Skeletal Muscle Growth Recovery in Rats Following Chronic Protein-Energy Malnutrition J. Nutr., April 1, 1999; 129(4): 828 - 837. [Abstract] [Full Text]

				C. J. Band and B. I. Posner Phosphatidylinositol 3'-Kinase and p70s6k Are Required for Insulin but Not Bisperoxovanadium 1,10-Phenanthroline (bpV(phen)) Inhibition of Insulin-like Growth Factor Binding Protein Gene Expression. EVIDENCE FOR MEK-INDEPENDENT ACTIVATION OF MITOGEN-ACTIVATED PROTEIN KINASE BY bpV(phen) J. Biol. Chem., January 3, 1997; 272(1): 138 - 145. [Abstract] [Full Text] [PDF]