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An -Subunit-Secreting Cell Line Derived from a Mouse Thyrotrope Tumor

Regulatory Biology Laboratory, The Salk Institute La Jolla, California 92037
Department of Biology, University of California San Diego, California 92093
Department of Medicine, University of Colorado Health Sciences Center Denver, Colorado 80262

Address requests for reprints to: Dr. Pamela L. Mellon, The Salk Institute, 10010 North Torrey Pines Road, La Jolla, California 92037.

Abstract

The anterior pituitary contains multiple distinct endocrine cell types that secrete individual hormones. To derive a pure cell culture population in which to study the regulation of the -subunit of TSH free of other hormones and cell types, we have developed a clonal continuous cell line from the transplantable thyrotrope tumor MGH101A. This cell line expresses -subunit mRNA, secretes -subunit protein, and has maintained a stable phenotype for over 3 yr in culture. However, as is the case for the transplantable tumor from which they are derived, these cells do not express the β-subunit of TSH or respond to TRH or thyroid hormone. We have used this cell line to investigate regulation of the -subunit mRNA by the second messengers, cAMP and phorbol esters, and by glucocorticoids. Phorbol esters increase -subunit mRNA levels significantly (3.5-fold), as does cAMP (1.8-fold). In contrast, glucocorticoids decrease mRNA levels from cAMP-induced or basal levels (2-fold). These cells should prove valuable for study of -subunit gene expression in an isolated renewable clonal cell culture system.

FOOTNOTES

This work was supported by NIH Grants HD-20377 and HD-23818 (to P.L.M.) and CA-47411 and DK-36843 (to E.C.R.).

^* Present address: Department of Biology, University of Virginia, Charlottesville, Virginia 22908.

Received for publication October 3, 1989. Revision received January 4, 1990. Accepted for publication January 9, 1990.

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