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Molecular Endocrinology Vol. 4, No. 8 1136-1143
doi:10.1210/mend-4-8-1136
Copyright © 1990 by the Endocrine Society.
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Expression of Two Forms of Prolactin Receptor in Rat Ovary and Liver

Mariko Shirota, Denis Banville, Suhad Ali, Christine Jolicoeur, Jean-Marie Boutin, Marc Edery, Jean Djiane and Paul A. Kelly

Laboratory of Molecular Endocrinology, McGill University, Royal Victoria Hospital Montreal, Quebec, Canada H3A 1A1
Biotechnology Research Institute, National Research Council Montreal, Canada H4P 2R2
Unité d'Endocrinologie Moléculaire Institut National de la Recherche Agronomique, CRJJ 78350 Jouy-en-Josas, France

Address requests for reprints to: Dr. Paul A. Kelly, Laboratory of Molecular Endocrinology, McGill University, Royal Victoria Hospital, 687 Pine Avenue West, Montreal, Quebec, Canada H3A 1A1.

Abstract

The screening of a size-selected cDNA library from the ovary revealed the existence of a second form of PRL receptor in the rat. The polypeptide sequence deduced from cDNAs has a much longer cytoplasmic domain (357 amino acids) than the form previously identified in the liver (57 amino acids). Nucleotide sequence analysis and comparison with rabbit, mouse, and human PRL receptor cDNAs suggests that the two forms of rat PRL receptor result from alternative splicing of a primary transcript. Complementary DNAs encoding the long form of the receptor were also found in a library prepared from estradiol-treated rat liver, although they represent a minor fraction of total PRL receptor cDNAs obtained from this tissue. DNA polymerase chain reaction amplification of cDNA confirmed the presence of the two receptor forms in both the ovary and liver. Northern analysis, using probes that specifically hybridize with either form of mRNA, indicates a major transcript of 1.8 kilobases (kb) in estradiol-treated liver, which encodes the receptor with a short cytoplasmic domain, while the long form of the receptor is encoded by mRNAs of 2.5 and 3 kb. In the ovary, a complex pattern of hybridization to multiple mRNAs (1.8–5.5 kb) is obtained with the probe specific to the long form, and essentially only a 5.5-kb mRNA is obtained with the probe specific to the short form. The predicted size of the mature form of the long PRL receptor (PRL-R2) is 591 amino acid residues. The cytoplasmic domain of this receptor shows strong overall sequence similarity with the corresponding residues of the rabbit (67%) and human (66%) PRL receptors and four regions of localized identity with the rabbit and human GH receptor.

FOOTNOTES

This work was supported in part by grants from the Medical Research Council of Canada and the NCI of Canada.

Received for publication March 6, 1990. Revision received May 11, 1990. Accepted for publication May 21, 1990.




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