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Independent Expression of the and β c-erbA Genes in Developing Rat Brain

Institute de Investigaciones Biomédicas, Consejo Superior Investigaciones Científicas Facultad de Medicina, Universidad Autónoma de Madrid 28029 Madrid, Spain
Instituto Cajal de Neurociencia, Consejo Superior Investigaciones Científicas 28002 Madrid, Spain
Departamento de Bioquímica, Facultad de Medicina, Universidad Complutense de Madrid 28040 Madrid, Spain
Departamento de Fisiología y Farmacología, Facultad de Medicina, Universidad de Cantabria 39011 Santander, Spain

Address requests for reprints to: Dr. Britt Mellström, Instituto Cajal, Consejo Superior Investigaciones Cientifícas, Avenue Doctor Arce 37, 28002 Madrid, Spain.

Abstract

Thyroid hormone is important for normal brain development. Cellular responses to thyroid hormone are mediated by multiple nuclear receptors, classified into - and β-subtypes. In the rat, expression of both the and β genes results in several translation products. By using cRNA probes common to transcripts or specific for -1 and β-1, we have studied the distribution of these transcripts in rat brain at different stages of development from embryonic day 14 to adult age by using in situ hybridization histochemistry. On embryonic day 14, the -1 mRNA is already widely expressed at a low level in the developing brain. The -1 mRNA is developmentally regulated and showed a peak in expression during the first 3 postnatal weeks in the cerebral cortex, amygdala, hippocampus, and cerebellum. The probe common to the transcripts detected a widespread distribution and high levels of these forms in the same regions throughout postnatal development. The level of β-1 mRNA before birth was low or undetectable. The β-1 transcript showed developmental regulation as well, with a high level at birth in the mitral cell layer of the olfactory bulb, accumbens nucleus, caudate, and hippocampal field CA1 and increasing levels in other regions later during development. Complementary expression of the and β forms was seen in the cerebral cortex and hippocampus. The differential temporal and spatial distribution as well as coexpression at comparable levels in certain brain regions suggest different roles for the c-erbA proteins during brain development and in the mature animal.

FOOTNOTES

This work has been supported by grants from Ministerio Education y Ciencia (to B.M.), Direccion General Investigacion Cientifica y Tecnica (PM88–0014) and Fundación Ramón Areces (to J.R.N.), and Fondo de Investigación Sanitaria 88/1454 and Direccion General Investigacion Científica y Tecnica (PM88–0006; to J.B.).

Received for publication February 7, 1991. Revision received June 11, 1991. Accepted for publication June 15, 1991.

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