| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
Molecular Endocrinology, Vol 6, 1797-1804, Copyright © 1992 by Endocrine Society
ARTICLES |
F Lezoualc'h, AH Hassan, P Giraud, JP Loeffler, SL Lee and BA Demeneix
Laboratoire de Physiologie Generale et Comparee, URA 90 CNRS, Museum National d'Histoire Naturelle, Paris, France.
Thyroid hormone, T3, is essential to the normal development and metabolism of vertebrates. Fine tuning of circulating levels of T3 is critical and involves feedback inhibition of the TRH and TSH genes by T3 at the hypothalamic and hypophyseal levels. However, the molecular basis of T3 inhibition of TRH gene expression in the hypothalamus is not known. The actions of T3 on target gene expression are mediated through nuclear receptor proteins, TR alpha and TR beta. To examine their effects on T3-dependent transcription from the rat TRH promoter, we used a gene transfer technique to express TR alpha and TR beta in cultured embryonic chick hypothalamic cells. Transcription from the TRH promoter construct transfected into these cultures was depressed in the presence of 10(-9) M T3. Cotransfecting TR alpha or TR beta activated transcription from the TRH promoter. However, only TR beta-dependent TRH transcription was differentially modulated by T3. Physiological concentrations of T3 decreased TR beta-dependent TRH transcription 4- fold. Thus, when T3 levels increase, TR beta mediates inhibition of TRH expression, a key step in down-regulating the hypophyseal-thyroid axis. This study demonstrates for the first time a T3-dependent differential regulation of the TRH promoter by TR beta and not TR alpha. Thus, the negative regulation of the TRH promoter in transiently transfected primary embryonic chick hypothalamic neurons provides a useful system for studying the molecular actions of thyroid hormone receptors.
This article has been cited by other articles:
 |
|
 |
|
  S. V H Grommen, L. Arckens, T. Theuwissen, V. M Darras, and B. De Groef Thyroid hormone receptor {beta}2 is strongly up-regulated at all levels of the hypothalamo-pituitary-thyroidal axis during late embryogenesis in chicken J. Endocrinol., March 1, 2008; 196(3): 519 - 528. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 E. J W Geven, F. Verkaar, G. Flik, and P. H M Klaren Experimental hyperthyroidism and central mediators of stress axis and thyroid axis activity in common carp (Cyprinus carpio L.) J. Mol. Endocrinol., December 1, 2006; 37(3): 443 - 452. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P.-Q. Yuan and H. Yang Hypothyroidism increases Fos immunoreactivity in cholinergic neurons of brain medullary dorsal vagal complex in rats Am J Physiol Endocrinol Metab, November 1, 2005; 289(5): E892 - E899. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
X. Shen, Q.-L. Li, G. A. Brent, and T. C. Friedman Regulation of regional expression in rat brain PC2 by thyroid hormone/characterization of novel negative thyroid hormone response elements in the PC2 promoter Am J Physiol Endocrinol Metab, January 1, 2005; 288(1): E236 - E245. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 S. M. Dupre, H. Guissouma, F. Flamant, I. Seugnet, T. S. Scanlan, J. D. Baxter, J. Samarut, B. A. Demeneix, and N. Becker Both Thyroid Hormone Receptor (TR){beta}1 and TR{beta}2 Isoforms Contribute to the Regulation of Hypothalamic Thyrotropin-Releasing Hormone Endocrinology, May 1, 2004; 145(5): 2337 - 2345. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 M. Nygard, G. M. Wahlstrom, M. V. Gustafsson, Y. M. Tokumoto, and M. Bondesson Hormone-Dependent Repression of the E2F-1 Gene by Thyroid Hormone Receptors Mol. Endocrinol., January 1, 2003; 17(1): 79 - 92. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
H. Guissouma, S. M. Dupre, N. Becker, E. Jeannin, I. Seugnet, B. Desvergne, and B. A. Demeneix Feedback on Hypothalamic TRH Transcription Is Dependent on Thyroid Hormone Receptor N Terminus Mol. Endocrinol., July 1, 2002; 16(7): 1652 - 1666. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 A. Rusch, L. Ng, R. Goodyear, D. Oliver, I. Lisoukov, B. Vennstrom, G. Richardson, M. W. Kelley, and D. Forrest Retardation of Cochlear Maturation and Impaired Hair Cell Function Caused by Deletion of All Known Thyroid Hormone Receptors J. Neurosci., December 15, 2001; 21(24): 9792 - 9800. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
N. Becker, I. Seugnet, H. Guissouma, S. M. Dupre, and B. A. Demeneix Nuclear Corepressor and Silencing Mediator of Retinoic and Thyroid Hormone Receptors Corepressor Expression Is Incompatible with T3-Dependent TRH Regulation Endocrinology, December 1, 2001; 142(12): 5321 - 5331. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 L. Ng, A. Rusch, L. L. Amma, K. Nordstrom, L. C. Erway, B. Vennstrom, and D. Forrest Suppression of the deafness and thyroid dysfunction in Thrb-null mice by an independent mutation in the Thra thyroid hormone receptor {alpha} gene Hum. Mol. Genet., November 1, 2001; 10(23): 2701 - 2708. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 K. Gauthier, M. Plateroti, C. B. Harvey, G. R. Williams, R. E. Weiss, S. Refetoff, J. F. Willott, V. Sundin, J.-P. Roux, L. Malaval, et al. Genetic Analysis Reveals Different Functions for the Products of the Thyroid Hormone Receptor {alpha} Locus Mol. Cell. Biol., July 15, 2001; 21(14): 4748 - 4760. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 P. M. Yen Physiological and Molecular Basis of Thyroid Hormone Action Physiol Rev, July 1, 2001; 81(3): 1097 - 1142. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
L. L. Amma, A. Campos-Barros, Z. Wang, B. Vennström, and D. Forrest Distinct Tissue-Specific Roles for Thyroid Hormone Receptors {beta} and {{alpha}}1 in Regulation of Type 1 Deiodinase Expression Mol. Endocrinol., March 1, 2001; 15(3): 467 - 475. [Abstract] [Full Text]
|
|
|
|
|
|
Q.-L. Li, E. Jansen, G. A. Brent, and T. C. Friedman Regulation of prohormone convertase 1 (PC1) by thyroid hormone Am J Physiol Endocrinol Metab, January 1, 2001; 280(1): E160 - E170. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
G. R. Williams Cloning and Characterization of Two Novel Thyroid Hormone Receptor beta Isoforms Mol. Cell. Biol., November 15, 2000; 20(22): 8329 - 8342. [Abstract] [Full Text]
|
|
|
|
|
|
H. Favre-Young, F. Dif, F. Roussille, B. A. Demeneix, P. A. Kelly, M. Edery, and A. de Luze Cross-Talk between Signal Transducer and Activator of Transcription (Stat5) and Thyroid Hormone Receptor-{beta} 1 (TR{beta}1) Signaling Pathways Mol. Endocrinol., September 1, 2000; 14(9): 1411 - 1424. [Abstract] [Full Text]
|
|
|
|
|
|
Q.-L. Li, E. Jansen, G. A. Brent, S. Naqvi, J. F. Wilber, and T. C. Friedman Interactions between the Prohormone Convertase 2 Promoter and the Thyroid Hormone Receptor Endocrinology, September 1, 2000; 141(9): 3256 - 3266. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Dace, G. Sarkissian, L. Schneider, C. Martin-El Yazidi, J. Bonne, A. Margotat, R. Planells, and J. Torresani Transient Expression of c-erbA{beta}1 Messenger Ribonucleic Acid and {beta}1 Thyroid Hormone Receptor Early in Adipogenesis of Ob 17 Cells Endocrinology, July 1, 1999; 140(7): 2983 - 2990. [Abstract] [Full Text]
|
|
|
|
|
|
R. E. Weiss, Y. Murata, K. Cua, Y. Hayashi, H. Seo, and S. Refetoff Thyroid Hormone Action on Liver, Heart, and Energy Expenditure in Thyroid Hormone Receptor {beta}-Deficient Mice Endocrinology, December 1, 1998; 139(12): 4945 - 4952. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 H. Guissouma, M. T. Ghorbel, I. Seugnet, T. Ouatas, and B. A. Demeneix Physiological regulation of hypothalamic TRH transcription in vivo is T3 receptor isoform specific FASEB J, December 1, 1998; 12(15): 1755 - 1764. [Abstract] [Full Text]
|
|
|
|
|
|
K.-h. Lin, X.-G. Zhu, H.-C. Hsu, S.-L. Chen, H.-y. Shieh, S.-T. Chen, P. McPhie, and S.-y. Cheng Dominant Negative Activity of Mutant Thyroid Hormone {alpha}1 Receptors from Patients with Hepatocellular Carcinoma Endocrinology, December 1, 1997; 138(12): 5308 - 5315. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
A. Farsetti, J. Lazar, M. Phyillaier, R. Lippoldt, A. Pontecorvi, and V. M. Nikodem Active Repression by Thyroid Hormone Receptor Splicing Variant {alpha}2 Requires Specific Regulatory Elements in the Context of Native Triiodothyronine-Regulated Gene Promoters Endocrinology, November 1, 1997; 138(11): 4705 - 4712. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
R. E. Weiss, D. Forrest, J. Pohlenz, K. Cua, T. Curran, and S. Refetoff Thyrotropin Regulation by Thyroid Hormone in Thyroid Hormone Receptor {beta}-Deficient Mice Endocrinology, September 1, 1997; 138(9): 3624 - 3629. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
S. G. Ball, M. Ikeda, and W. W. Chin Deletion of the Thyroid Hormone {beta}1 Receptor Increases Basal and Triiodothyronine-Induced Growth Hormone Messenger Ribonucleic Acid in GH3 Cells Endocrinology, August 1, 1997; 138(8): 3125 - 3132. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
B. Huo, B. Dozin, and V. M. Nikodem Identification of a Nuclear Protein from Rat Developing Brain as Heterodimerization Partner with Thyroid Hormone Receptor-{beta} Endocrinology, August 1, 1997; 138(8): 3283 - 3289. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 X.-G. Zhu, P. McPhie, K.-H. Lin, and S.-Y. Cheng The Differential Hormone-dependent Transcriptional Activation of Thyroid Hormone Receptor Isoforms Is Mediated by Interplay of Their Domains J. Biol. Chem., April 4, 1997; 272(14): 9048 - 9054. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 N. Oudrhiri, J.-P. Vigneron, M. Peuchmaur, T. Leclerc, J.-M. Lehn, and P. Lehn Gene transfer by guanidinium-cholesterol cationic lipids into airway epithelial cells in vitro and in vivo PNAS, March 4, 1997; 94(5): 1651 - 1656. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
T. Satoh, M. Yamada, T. Iwasaki, and M. Mori Negative Regulation of the Gene for the Preprothyrotropin-releasing Hormone from the Mouse by Thyroid Hormone Requires Additional Factors in Conjunction with Thyroid Hormone Receptors J. Biol. Chem., November 1, 1996; 271(44): 27919 - 27926. [Abstract] [Full Text] [PDF]
|
|
|
|
|
|
F. Lezoualc'h, I. Seugnet, A. L. Monnier, J. Ghysdael, J.-P. Behr, and B. A. Demeneix Inhibition of Neurogenic Precursor Proliferation by Antisense [IMAGE] Thyroid Hormone Receptor Oligonucleotides J. Biol. Chem., May 19, 1995; 270(20): 12100 - 12108. [Abstract] [Full Text] [PDF]
|
|
|
|
 |
|
 G. A. Brent The Molecular Basis of Thyroid Hormone Action N. Engl. J. Med., September 29, 1994; 331(13): 847 - 853. [Full Text]
|
|
| HOME | HELP | FEEDBACK | SUBSCRIPTIONS | ARCHIVE | SEARCH | TABLE OF CONTENTS |
| Endocrinology | Endocrine Reviews | J. Clin. End. & Metab. |
| Molecular Endocrinology | Recent Prog. Horm. Res. | All Endocrine Journals |
