help button home button Endocrine Society Molecular Endocrinology ENDO 08 Sessions Library
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS

Molecular Endocrinology Vol. 6, No. 12 2114-2122
doi:10.1210/me.6.12.2114
Copyright © 1992 by the Endocrine Society.
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Right arrow Citation Map
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow Request Copyright Permission
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Van Patten, S. M.
Right arrow Articles by Maurer, R. A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Van Patten, S. M.
Right arrow Articles by Maurer, R. A.

Molecular Endocrinology, Vol 6, 2114-2122, Copyright © 1992 by Endocrine Society


ARTICLES

The alpha- and beta-isoforms of the inhibitor protein of the 3',5'- cyclic adenosine monophosphate-dependent protein kinase: characteristics and tissue- and developmental-specific expression

SM Van Patten, P Howard, DA Walsh and RA Maurer
Department of Biological Chemistry, School of Medicine, University of California, Davis 95616.

The inhibitor protein (PKI) of the cAMP-dependent protein kinase was first characterized from rabbit skeletal muscle. More recently a form of PKI was isolated and cloned from rat testis which shares relatively limited amino acid sequence with the rabbit skeletal muscle form. We have now isolated a cDNA from rat brain which encodes a protein corresponding to the rabbit skeletal muscle PKI. This establishes the presence of the "skeletal muscle" and "testis" proteins in the same species and therefore that they clearly represent distinct isoforms. We have also demonstrated that the isoform from testis, like the skeletal muscle isoform, is specific for the cAMP-dependent protein kinase and that it is able to inhibit this enzyme when expressed in cultured JEG-3 cells. Both forms contain the five specific amino acid recognition determinants which have been shown to be required for high affinity binding to the protein kinase catalytic site, although there is some noted lack of conservation of codons used for these residues. Overall, the two rat isoforms are only 41% identical at the amino acid level and 46% at the level of coding nucleotides. We propose that the rabbit skeletal muscle and rat testis forms be designated PKI alpha and PKI beta, respectively. Using Northern blot analysis, we have examined the tissue distribution of the two forms in the rat and their relative expression during development. In the adult rat, mRNA of the PKI alpha species is highest in muscle (both skeletal and cardiac) and brain (cortex and cerebellum).(ABSTRACT TRUNCATED AT 250 WORDS)


This article has been cited by other articles:


Home page
Mol. Endocrinol.Home page
M. H. Al-Dhaheri and B. G. Rowan
Protein Kinase A Exhibits Selective Modulation of Estradiol-Dependent Transcription in Breast Cancer Cells that Is Associated with Decreased Ligand Binding, Altered Estrogen Receptor {alpha} Promoter Interaction, and Changes in Receptor Phosphorylation
Mol. Endocrinol., February 1, 2007; 21(2): 439 - 456.
[Abstract] [Full Text] [PDF]


Home page
DevelopmentHome page
D. Sakai, T. Suzuki, N. Osumi, and Y. Wakamatsu
Cooperative action of Sox9, Snail2 and PKA signaling in early neural crest development
Development, April 1, 2006; 133(7): 1323 - 1333.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
X. Chen, J.-C. Dai, S. A. Orellana, and E. M. Greenfield
Endogenous Protein Kinase Inhibitor {gamma} Terminates Immediate-early Gene Expression Induced by cAMP-dependent Protein Kinase (PKA) Signaling: TERMINATION DEPENDS ON PKA INACTIVATION RATHER THAN PKA EXPORT FROM THE NUCLEUS
J. Biol. Chem., January 28, 2005; 280(4): 2700 - 2707.
[Abstract] [Full Text] [PDF]


Home page
J. Pharmacol. Exp. Ther.Home page
K. K. Meja, M. C. Catley, L. M. Cambridge, P. J. Barnes, H. Lum, R. Newton, and M. A. Giembycz
Adenovirus-Mediated Delivery and Expression of a cAMP-Dependent Protein Kinase Inhibitor Gene to BEAS-2B Epithelial Cells Abolishes the Anti-Inflammatory Effects of Rolipram, Salbutamol, and Prostaglandin E2: A Comparison with H-89
J. Pharmacol. Exp. Ther., May 1, 2004; 309(2): 833 - 844.
[Abstract] [Full Text] [PDF]


Home page
Biol. Reprod.Home page
K.-F. Lee, W. S.B. Yeung, J. F.C. Chow, C. K. Shum, and J. M. Luk
Different Testicular Gene Expression Patterns in the First Spermatogenic Cycle of Postnatal and Vitamin A-Deficient Rat Testis
Biol Reprod, April 1, 2004; 70(4): 1010 - 1017.
[Abstract] [Full Text] [PDF]


Home page
EndocrinologyHome page
J. J. Tremblay, F. Hamel, and R. S. Viger
Protein Kinase A-Dependent Cooperation between GATA and CCAAT/Enhancer-Binding Protein Transcription Factors Regulates Steroidogenic Acute Regulatory Protein Promoter Activity
Endocrinology, October 1, 2002; 143(10): 3935 - 3945.
[Abstract] [Full Text] [PDF]


Home page
DevelopmentHome page
M. Kawakami and N. Nakanishi
The role of an endogenous PKA inhibitor, PKI{alpha}, in organizing left-right axis formation
Development, July 1, 2001; 128(13): 2509 - 2515.
[Abstract] [Full Text] [PDF]


Home page
Mol. Cell. Biol.Home page
M. Belyamani, E. A. Gangolli, and R. L. Idzerda
Reproductive Function in Protein Kinase Inhibitor-Deficient Mice
Mol. Cell. Biol., June 15, 2001; 21(12): 3959 - 3963.
[Abstract] [Full Text]


Home page
Mol. Cell. Biol.Home page
E. A. Gangolli, M. Belyamani, S. Muchinsky, A. Narula, K. A. Burton, G. S. McKnight, M. D. Uhler, and R. L. Idzerda
Deficient Gene Expression in Protein Kinase Inhibitor alpha Null Mutant Mice
Mol. Cell. Biol., May 15, 2000; 20(10): 3442 - 3448.
[Abstract] [Full Text]


Home page
Biol. Reprod.Home page
D.A. Harrison, D.W. Carr, and S. Meizel
Involvement of Protein Kinase A and A Kinase Anchoring Protein in the Progesterone-Initiated Human Sperm Acrosome Reaction
Biol Reprod, March 1, 2000; 62(3): 811 - 820.
[Abstract] [Full Text]


Home page
Mol. Endocrinol.Home page
I. J. Gonzalez-Robayna, T. N. Alliston, P. Buse, G. L. Firestone, and J. S. Richards
Functional and Subcellular Changes in the A-Kinase-Signaling Pathway: Relation to Aromatase and Sgk Expression during the Transition of Granulosa Cells to Luteal Cells
Mol. Endocrinol., August 1, 1999; 13(8): 1318 - 1337.
[Abstract] [Full Text]


Home page
Proc. Natl. Acad. Sci. USAHome page
B. H. Chang, S. Mukherji, and T. R. Soderling
Characterization of a calmodulin kinase II inhibitor protein in brain
PNAS, September 1, 1998; 95(18): 10890 - 10895.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
H. Yao, R. D. York, A. Misra-Press, D. W. Carr, and P. J. S. Stork
The Cyclic Adenosine Monophosphate-dependent Protein Kinase (PKA) Is Required for the Sustained Activation of Mitogen-activated Kinases and Gene Expression by Nerve Growth Factor
J. Biol. Chem., April 3, 1998; 273(14): 8240 - 8247.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
P. Kumar, S. M. Van Patten, and D. A. Walsh
Multiplicity of the beta  Form of the cAMP-dependent Protein Kinase Inhibitor Protein Generated by Post-translational Modification and Alternate Translational Initiation
J. Biol. Chem., August 8, 1997; 272(32): 20011 - 20020.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. M. Van Patten, L. F. Donaldson, M. P. McGuinness, P. Kumar, A. Alizadeh, M. D. Griswold, and D. A. Walsh
Specific Testicular Cellular Localization and Hormonal Regulation of the PKIalpha and PKIbeta Isoforms of the Inhibitor Protein of the cAMP-dependent Protein Kinase
J. Biol. Chem., August 8, 1997; 272(32): 20021 - 20029.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. P. Collins and M. D. Uhler
Characterization of PKIgamma , a Novel Isoform of the Protein Kinase Inhibitor of cAMP-dependent Protein Kinase
J. Biol. Chem., July 18, 1997; 272(29): 18169 - 18178.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
D. M. Gamm and M. D. Uhler
Isoform-specific Differences in the Potencies of Murine Protein Kinase Inhibitors Are Due to Nonconserved Amino-terminal Residues
J. Biol. Chem., March 31, 1995; 270(13): 7227 - 7232.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
W. Wen, S. S. Taylor, and J. L. Meinkoth
The Expression and Intracellular Distribution of the Heat-stable Protein Kinase Inhibitor Is Cell Cycle Regulated
J. Biol. Chem., February 3, 1995; 270(5): 2041 - 2046.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
S. A. Orellana and C. Marfella-Scivittaro
Distinctive Cyclic AMP-dependent Protein Kinase Subunit Localization Is Associated with Cyst Formation and Loss of Tubulogenic Capacity in Madin-Darby Canine Kidney Cell Clones
J. Biol. Chem., July 7, 2000; 275(28): 21233 - 21240.
[Abstract] [Full Text] [PDF]


Home page
J. Biol. Chem.Home page
H. Pincas, J.-N. Laverriere, and R. Counis
Pituitary Adenylate Cyclase-activating Polypeptide and Cyclic Adenosine 3',5'-Monophosphate Stimulate the Promoter Activity of the Rat Gonadotropin-releasing Hormone Receptor Gene via a Bipartite Response Element in Gonadotrope-derived Cells
J. Biol. Chem., June 22, 2001; 276(26): 23562 - 23571.
[Abstract] [Full Text] [PDF]


Home page
Am. J. Physiol. Cell Physiol.Home page
H. Lum, Z. Hao, D. Gayle, P. Kumar, C. E. Patterson, and M. D. Uhler
Vascular endothelial cells express isoforms of protein kinase A inhibitor
Am J Physiol Cell Physiol, January 1, 2002; 282(1): C59 - C66.
[Abstract] [Full Text] [PDF]




HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Endocrinology Endocrine Reviews J. Clin. End. & Metab.
Molecular Endocrinology Recent Prog. Horm. Res. All Endocrine Journals
Copyright © 1992 by The Endocrine Society